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Journal of Natural Medicines

Erschienen in:

09.01.2019 | Original Paper

Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels

verfasst von: Weicheng Wang, Souichi Nakashima, Seikou Nakamura, Yoshimi Oda, Hisashi Matsuda

Erschienen in: Journal of Natural Medicines | Ausgabe 3/2019

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Abstract

Auriculataoside A, an anthracenone dimer glycoside isolated from Cassia auriculata seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC₅₀ value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.

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Wang C, Wu X, Chen M, Duan W, Sun L, Yan M, Zhang L (2007) Emodin induces apoptosis through caspase 3-dependent pathway in HK-2 cells. Toxicology 231:120–128CrossRef

Chen HC, Hsieh WT, Chang WC, Chung JG (2004) Aloe-emodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells. Food Chem Toxicol 42:1251–1257CrossRef

Pecere T, Gazzola MV, Mucignat C, Parolin C, Vecchia FD, Cavaggioni A, Basso G, Diaspro A, Salvato B, Carli M, Giorgio Palù (2007) Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Res 60:2800–2804

Wang W, Zhang Y, Nakashima S, Nakamura S, Wang T, Yoshikawa M, Matsuda H (2019) Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds. J Nat Med. doi: https://doi.org/10.1007/s11418-018-01276-2

Nakashima S, Oda Y, Nakamura S, Liu J, Onishi K, Kawabata M, Miki H, Himuro Y, Yoshikawa M, Matsuda H (2015) Inhibitors of melanogenesis in B16 melanoma 4A5 cells from flower buds of Lawsonia inermis (Henna). Bioorg Med Chem Lett 25:2701–2706

Matsuda H, Nakashima S, Oda Y, Nakamura S, Yoshikawa M (2009) Melanogenesis inhibitors from the rhizomes of Alpinia officinarum in B16 melanoma cells. Bioorg Med Chem 17:6048–6053CrossRef

Nakashima S, Oda Y, Kishimoto M, Uno M, Aoki M, Nakamura S, Tanaka H, Matsuda H (2018) Anti-invasive activity of Lawsonia inermis branch and its potential target protein. Nat Prod Commun 13:1337–1340

Nakashima S, Matsuda H, Oda Y, Nakamura S, Xu F, Yoshikawa M (2010) Melanogenesis inhibitors from the desert plant Anastatica hierochuntica in B16 melanoma cells. Bioorg Med Chem 18:2337–2345CrossRef

Nakashima S, Matsuda H, Kurume A, Oda Y, Nakamura S, Yamashita M, Yoshikawa M (2010) Cucurbitacin E as a new inhibitor of cofilin phosphorylation in human leukemia U937 cells. Bioorg Med Chem Lett 20:2994–2997CrossRef

10.

Jin J, Gong J, Yin T, Lu Y, Xia J, Xie Y, Di Y, He L, Guo J, Sun J, Noteborn MHM, Qu S (2011) PTD4-apoptin protein and dacarbazine show a synergistic antitumor effect on B16-F1 melanoma in vitro and in vivo. Eur J Pharmacol 654:17–25CrossRef

11.

Li J, Yang K, Ke X, Du R, Zhang X, Zhang Q (2014) In vitro and in vivo antitumor efficacy of CLA-PTX on B16-F10 melanoma cells. J Chin Pharm Sci 23:46–53

12.

Shenoy VS, Gude RP, Nikam Y, Murthy RSR (2017) In vitro cytotoxic potential of paclitaxel-encapsulated lipid-based drug delivery systems. Austin J Lung Cancer Res 2:1011

13.

Liu M, Bi F, Zhou X, Zheng Y (2012) Rho GTPase regulation by miRNAs and covalent modifications. Trends Cell Biol 22:365–373CrossRef

14.

Mackay DJG, Hall A (1998) Rho GTPases. J Bio Chem 273:20685–20688CrossRef

15.

Smit MA, Maddalo G, Greig K, Raaijmakers LM, Possik PA, van Breukelen B, Cappadona S, Heck AJ, Altelaar AF, Peeper DS (2014) ROCK1 is a potential combinatorial drug target for BRAF mutant melanoma. Mol Syst Biol 10:772–773CrossRef

16.

Coleman ML, Marshall CJ, Olson MF (2004) Ras and Rho GTPases in G1-phase cell-cycle regulation. Nat Rev Mol Cell Biol 5:355–366CrossRef

17.

Jaffe AB, Hall A (2005) Rho GTPases: biochemistry and biology. Cell Dev Biol 21:247–269CrossRef

18.

Etienne-Manneville S, Hall A (2002) Rho GTPases in cell biology. Nature 420:629–635CrossRef

19.

Vega FM, Ridley AJ (2008) Rho GTPases in cancer cell biology. Nucl Dyn Cytoskel Signal 582:2093–2101

20.

Sadok A, McCarthy A, Caldwell J, Collins I, Garrett MD, Yeo M, Hooper S, Sahai E, Kuemper S, Mardakheh FK, Marshall CJ (2015) Rho kinase inhibitors block melanoma cell migration and inhibit metastasis. Cancer Res 75:2272–2284CrossRef

21.

Sahai E, Marshall CJ (2003) Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis. Nat Cell Biol 5:711–719CrossRef

22.

Larue L, Delmas V (2006) The WNT/beta-catenin pathway in melanoma. Front Biosci 11:733–742CrossRef

23.

Schlessinger K, Hall A, Tolwinski N (2009) Wnt signaling pathways meet Rho GTPases. Genes Dev 23:265–277CrossRef

24.

Giles RH, van Es JH, Clevers H (2003) Caught up in a Wnt storm: Wnt signaling in cancer. Biochim Biophys Acta 1653:1–24PubMed

25.

Sinnberg T, Menzel M, Ewerth D, Sauer B, Schwarz M, Schaller M, Garbe C, Schittek B (2011) β-Catenin signaling increases during melanoma progression and promotes tumor cell survival and chemoresistance. PLoS ONE 6:e23429CrossRef

26.

Baldus SE, Mönig SP, Huxel S, Landsberg S, Hanisch FG, Engelmann K, Schneider PM, Thiele J, Hölscher AH, Dienes HP (2004) MUC1 and nuclear beta-catenin are coexpressed at the invasion front of colorectal carcinomas and are both correlated with tumor prognosis. Clin Cancer Res 10:2790–2796CrossRef

27.

Sun QY, Schatten H (2006) Regulation of dynamic events by microfilaments during oocyte maturation and fertilization. Reproduction 131:193–205CrossRef

28.

Schmandke A, Strittmatter SM (2007) ROCK and Rho: biochemistry and neuronal functions of Rho-associated protein kinases. Neuroscientist 13:454–469CrossRef

29.

Samuel MS, Lopez JI, McGhee EJ, Croft DR, Strachan D, Timpson P, Munro J, Schröder E, Zhou J, Brunton VG, Barker N, Clevers H, Sansom OJ, Anderson KI, Weaver VM, Olson MF (2011) Actomyosin-mediated cellular tension drives increased tissue stiffness and β-catenin activation to induce interfollicular epidermal hyperplasia and tumor growth. Cancer Cell 19:776–791CrossRef

30.

Strub T, Giuliano S, Ye T, Bonet C, Keime C, Kobi D, Gras SL, Cormont M, Ballotti R, Bertolotto C, Davidson I (2011) Essential role of microphthalmia transcription factor for DNA replication, mitosis and genomic stability in melanoma. Oncogene 30:2319–2332CrossRef

Titel: Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels
verfasst von: Weicheng Wang
Souichi Nakashima
Seikou Nakamura
Yoshimi Oda
Hisashi Matsuda
Publikationsdatum: 09.01.2019
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines / Ausgabe 3/2019
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-01278-0

Springer Medizin

Abstract

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