nach oben

Erschienen in:

01.03.2008 | Original Research

Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

verfasst von: Paulo Henrique Braz-Silva, Nathalie Pepe Medeiros de Rezende, Karem Lopez Ortega, Raimunda Telma de Macedo Santos, Marina Helena Cury Gallottini de Magalhães

Erschienen in: Head and Neck Pathology | Ausgabe 1/2008

Einloggen, um Zugang zu erhalten

Abstract

Histopathological findings in cases of hairy leukoplakia (HL) are not exclusive to this lesion. A total of 36 tissue samples from patients previously diagnosed with HL based solely on morphological aspects were used in this study. Our purpose was to confirm the presence of Epstein–Barr virus (EBV) in these tissue samples by in situ hybridization (ISH), and to compare the detection of EBV with specific histopathological findings observed in each case. Among the 36 specimens, 80.55% were EBV positive, confirming the previous clinical and histhophatological diagnosis. None of the histopathological findings analyzed correlated with the presence or absence of EBV. This shows that a definitive diagnosis of HL cannot be established based on histopathological findings alone. Because there are many important implications on the establishment of definitive diagnosis of HL, the detection of EBV by ISH is obligatory.

Faulkner GC, Krajewski AS, Crawford DH. The ins and outs of EBV infection. Trends Microbiol 2000;8(4):185–9.PubMedCrossRef

Rickinson AB, Kieff E. Epstein-Barr virus. Fields virology, 3rd ed. Philadelphia: Lippincott-Raven Publishers; 1996.

Cruchley AT, Murray PG, Niedobitek G, Reynolds GM, Williams DM, Young LS. The expression of the Epstein-Barr virus nuclear antigen (EBNA-I) in oral hairy leukoplakia Oral Dis 1997;3(Suppl 1):S177–S9.PubMed

Walling DM, Etienne W, Ray AJ, Flaitz CM, Nichols CM. Persistence and transition of Epstein-Barr virus genotypes in the pathogenesis of oral hairy leukoplakia. J Infect Dis 2004;190:387–95.PubMedCrossRef

Walling DM, Flaitz CM, Adler-Storthz K, Nichols CM. A non-invasive technique for studying oral epithelial Epstein-Barr virus infection and disease. Oral Oncol 2003;13:436–44.CrossRef

Raab-Traub N, Webster-Cyriaque J. Epstein-Barr virus infection and expression in oral lesions Oral Dis 1997;3(Suppl 1):S164–S70.PubMed

Greenspan D, Greenspan JS, Conant M, Petersen V, Silverman S Jr, De Souza Y. Oral hairy leukoplakia in male homosexuals: evidence of association with both pappilomavirus and a herpes-group virus. Lancet 1984;2(8407):831–4.PubMedCrossRef

Greenspan JS, Greenspan D, Lennette ET, Abrams DI, Conant MA, Petersen V, et al. Replication of Epstein-Barr virus within the epithelial cells of oral “hairy” leukoplakia, an AIDS associated lesion. New Eng J Med 1985;313:1564–71.PubMedCrossRef

Walling DM, Flaitz CM, Nichols M. Epstein-Barr virus replication in oral hairy leukoplakia: response, persistence, and resistance to treatment with valacyclovir. J Infect Dis 2003;188:883–90.PubMedCrossRef

10.

Greenspan JS, Greenspan D. Oral hairy leukoplakia: diagnosis and management. Oral Surg Oral Med Oral Pathol 1989;67:396–403.PubMedCrossRef

11.

Greenspan D, Greenspan JS, De Souza YG, Levy JA, Ungar AM. Oral hairy leukoplakia in an HIV-negative renal transplant recipient. J Oral Pathol Med 1989;18:32–4.PubMedCrossRef

12.

Macleod RI, Long LQ, Soames JV. Oral hairy leukoplakia in an HIV-negative renal transplant patient. Br Dent J 1990;169:208–9.PubMed

13.

Seymour PA, Thomason JM, Nolan A. Oral lesions in organ transplant patients. J Oral Pathol Med 1997;26:297–304.PubMedCrossRef

14.

Migliorati CA, Jones AC, Baughman PA. Use of cytology in the diagnosis of hairy leukoplakia. Oral Surg Oral Med Oral Pathol 1993;76(6):704–10.PubMedCrossRef

15.

Triantos D, Porter SR, Scully C, Teo CG. Oral hairy leukoplakia: clinicopathologic features, pathogenesis, diagnosis, and clinical significance. Clin Infect Dis 1997;25:1392–6.PubMedCrossRef

16.

Walling DM, Flaitz K, Hosein FG, Montes-Walters M, Nichols CM. Effect of Epstein-Barr virus replication on Langerhans cells in pathogenesis of oral hairy leukoplakia. J Infect Dis 2004;184:1656–63.CrossRef

17.

Shiboski CH. Epidemiology of HIV-related oral manifestations in women: a review. Oral Dis 1997;3(Suppl 1):18–27.

18.

De Souza Y, Greenspan D, Hammer M. Demonstration od Epstein-Barr virus DNA in the epithelial cells of hairy leukoplakia. J Dent Res 1986;65:765.

19.

EC-Clearinghouse on Oral Problems and Related to HIV Infection and WHO Collaborating Centre on Oral Manifestations of the Immunodeficiency Virus. Classification and diagnostic criteria for oral lesions in HIV infection. J Oral Pathol Med 1993;22(7):289–91.

20.

Greenspan JS, De Souza YG, Regezi JA, Daniels TE, Greenspan D, MacPhail LA, et al. Comparison of cytopathic changes in oral hairy leukoplakia with in situ hybridization for EBV DNA. Oral Dis 1998;4:95–9.PubMedCrossRef

21.

Crawford DH. Biology and disease associations of Epstein-Barr virus Phil Trans R Soc Lond B 2001;356:461–73.

22.

Schmidt-Westhausen A, Gelderblom HR, Neuhaus P, Reichart PA. Epstein-Barr virus in lingual epithelium of liver transplant patients. J Oral Pathol Med 1993;22:274–6.PubMedCrossRef

23.

Mabruk MJEMF, Flint SR, Coleman DC, Sheils O, Toner M, Atkins GJ. A rapid microwave-in situ hybridization method for the definitive diagnosis of oral hairy leukoplakia: comparison with immunohistochemistry. J Oral Pathol Med 1996;25:170–6.PubMedCrossRef

24.

Mabruk MJEMF, Flint SR, Toner M, Balluz I, Coleman D, Sullivan D, et al. In situ hybridization and the polymerase chain reaction (PCR) in the analysis of biopsies and exfoliative cytology specimens for definitive diagnosis of oral hairy leukoplakia (OHL). J Oral Pathol Med 1994;23:302–8.PubMedCrossRef

25.

Walling DM, Ling PD, Gordadze AV, Montes-Walters M, Flaitz CM, Nichols CM. Expression of Epstein-Barr virus latent genes in oral epithelium: determinants of the pathogenesis of oral hairy leukoplakia. J Infect Dis 2004;190:396–9.PubMedCrossRef

26.

Braz-Silva PH, Ortega KL, Rezende NP, Nunes FD, Magalhães MHCG. Detection of Epstein-Barr virus (EBV) in the oral mucosa of renal transplant patients. Diagn Cytopathol 2006;34(1):24–8.PubMedCrossRef

27.

Southam JC, Felix DH, Wray D, Cubie HA. Hairy leukoplakia—a histological study. Histopathol 1991;19:63–7.CrossRef

28.

Schiodt M, Greenspan D, Daniels TE, Greenspan JS. Clinical and histologic spectrum or oral hairy leukoplakia. Oral Surg Oral Med Oral Pathol 1987;64:716–20.PubMedCrossRef

29.

Fraga-Fernández J, Benito C, Lizaldez EB. Oral hairy leukoplakia: a histopathologic study of 32 cases. Am J Dermatol 1990;12(6):571–8.CrossRef

30.

Reichart PA, Langford A, Gelderblom HR, Pohle HD, Becker J, Wolf H. Oral hairy leukoplakia: observations in 95 cases ans review of the literature. J Oral Pathol Med 1989;18:410–5.PubMedCrossRef

31.

Eyeson JD, Tenant-Flowers M, Cooper DJ, Johnson NW, Warnakulasuriya KAAS. Oral manifestations of an HIV positive cohort in the era of highly active anti-retroviral therapy (HAART) in south London. J Oral Pathol Med 2002;31:169–74.PubMedCrossRef

32.

Tappuni AR, Flemming GJP. The effect of antiretroviral therapy on the prevalence of oral manifestations in HIV-infected patients: A UK study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;92:623–8.PubMedCrossRef

33.

Marcus M, Maida CA, Freed JR, Younai F, Coulter ID, Der-Martirosian C, et al. Oral white patches in a national sample of medical HIV patients in the era of HAART. Community Dent Oral Epidemiol 2005;33:99–106.PubMedCrossRef

34.

Ramos-Gomez FJ, Flaitz C, Catapano P, Murray P, Milnes AR, Dorenbaum A. Classification, diagnostic criteria, and treatment recomendations for orofacial manifestations in HIV-infected pediatric patients. J Clin Pediatr Dent 1999;23(2):85–96.PubMed

35.

Fisher DA, Daniels TE, Greenspan JS. Oral hairy leukoplakia unassociated with human immunodeficiency virus: pseudo oral hairy leukoplakia. J Am Acad Dermatol 1992;27(2 Pt 1):257–8.PubMed

36.

Green TL, Greenspan JL, Greenspan D, De Souza YG. Oral lesions mimicking hairy leukoplakia: a diagnostic dilemma. Oral Surg Oral Med Oral Pathol 1989;67(4):422–6.PubMedCrossRef

37.

Braz-Silva PH, Ortega KL, Resende NPM, Magalhães MHCG. Detection of Epstein-Barr virus (EBV) by in situ hybridization in lesions like oral hairy leukoplakia. Mod Pathol 2006;19(Suppl 3):150.

Titel: Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia
verfasst von: Paulo Henrique Braz-Silva
Nathalie Pepe Medeiros de Rezende
Karem Lopez Ortega
Raimunda Telma de Macedo Santos
Marina Helena Cury Gallottini de Magalhães
Publikationsdatum: 01.03.2008
Verlag: Humana Press Inc
Erschienen in: Head and Neck Pathology / Ausgabe 1/2008
Elektronische ISSN: 1936-0568
DOI: https://doi.org/10.1007/s12105-007-0039-9

Neu im Fachgebiet Pathologie

Open Access 15.04.2024 | Biomarker | Schwerpunkt: Next Generation Pathology

Springer Medizin

Detection of the Epstein–Barr Virus (EBV) by In situ Hybridization as Definitive Diagnosis of Hairy Leukoplakia

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2008

Lipopolysaccharide-Squamous Cell Carcinoma-Monocyte Interactions Induce Cancer-Supporting Factors Leading to Rapid STAT3 Activation

Epithelioid Hemangioendothelioma of the Head and Neck: Role of Podoplanin in the Differential Diagnosis

Multiple Malignant Salivary Gland Neoplasms: Mucoepidermoid Carcinoma of Palate and Adenoid Cystic Carcinoma of Floor of Mouth

Estrogen and Progesterone Receptor and p53 Gene Expression in Adenoid Cystic Cancer

Sclerosing Polycystic Adenosis of the Buccal Mucosa

Hamartomatous Angiolipoma of the Parotid Gland (Sialoangiolipoma)

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie