nach oben

Erschienen in:

01.03.2011 | Original Paper

Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors

verfasst von: Márcia Gaiger de Oliveira, Isabel da Silva Lauxen, Anna Cecília Moraes Chaves, Pantelis Varvaki Rados, Manoel Sant’Ana Filho

Erschienen in: Head and Neck Pathology | Ausgabe 1/2011

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to evaluate the biological profile of odontogenic epithelium by immunolabeling of epidermal growth factor receptor (EGFR), Ki-67 and survivin in keratocystic odontogenic tumors (KOT), dentigerous cysts (DC), and pericoronal follicles (PF). Immunohistochemical analysis was performed in 13 KOTs, 14 DCs and 9 PFs. Immunolabeling was analyzed in the basal and suprabasal layers of KOTs and DCs, and in the islands of odontogenic epithelium and/or reduced enamel epithelium of PFs. KOTs showed the highest proliferation rate among the three groups, mainly in suprabasal layers. EGFR immunolabeling was observed mainly in the cytoplasm in basal and suprabasal layers of KOTs and in the suprabasal layer of DCs. Immunolabeling in both membrane and cytoplasm was greater in PFs. In PFs, membrane-only staining was observed. Survivin immunolabeling showed a greater percentage of positive cells (scoring +++) in the suprabasal layer of KOTs. In DCs, both layers showed similar percentages of cells scoring +++; PFs showed the highest percentage of these cells. In KOTs, epithelial cells showed stimulus-independent neoplastic proliferative characteristics, suggesting the presence of a suprabasal proliferative compartment, maintained by inhibition of apoptosis. In DCs, the basal layer seemed to proliferate in response to stimulus. Although PFs showed low proliferative activity, the expression of EGFR indicates that some cells have a high capacity to respond to stimuli, which could probably explain the origin of odontogenic lesions.

Curran AE, Damm DD, Drummond JF. Pathologically significant pericoronal lesions in adults: histopathologic evaluation. J Oral Maxillofac Surg. 2002;60:613–7.CrossRefPubMed

Aldelsperger J, Campbell JH, Coates DB, et al. Early soft tissue pathosis associated with impacted third molars without pericoronal radiolucency. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000;89:402–6.CrossRef

Baumgart SC, Lauxen IS, Sant’Ana Filho M, et al. Epidermal growth factor receptor distribution in pericoronal follicles: relationship with the origin of odontogenic cysts and tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:240–5.CrossRef

Edmatsu M, Kumanoto H, Ooya K, et al. Apoptosis-related factors in the epithelial components of pericoronal follicles and dentigerous cysts associated with impacted third molars of the mandible. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:17–23.CrossRef

Payeras MR, Sant’Ana Filho M, Lauxen IS, et al. Quantitative analysis of argyrophilic nucleolar organizer regions and epidermal growth factor receptor in ameloblastomas. J Oral Pathol Med. 2007;36(2):99–104.CrossRefPubMed

Kramer IRH, Pindborg JJ, Shear M. Histological typing of odontogenic tumours. Berlin, Germany: Springer; 1992.

Philipsen HP. Keratocyst odontogenic tumour. In: Barnes L, Eveson JW, Reichart P, Sidransky D, editors. World Health Organization classification of tumours. Lyon, France: IARC Press; 2005.

Slootweg PJ. p53 protein and Ki-67 reactivity in epithelial odontogenic lesions: an immunohistochemical study. J Oral Pathol Med. 1995;24:393–7.CrossRefPubMed

Li TJ, Browne RM, Prime SS, et al. p53 expression in odontogenic keratocyst epithelium. J Oral Pathol Med. 1996;25:249–55.CrossRefPubMed

10.

Stoelinga PJW. Etiology and pathogenesis of keratocyst: natural history and immunohistochemistry. Oral Maxillofac Surg Clin North Am. 2003;15:317–24.CrossRefPubMed

11.

Kichi E, Enokiya Y, Muramatsu T, et al. Cell proliferation, apoptosis and apoptosis-related factors in odontogenic keratocysts and in dentigerous cysts. J Oral Pathol Med. 2005;34:280–6.CrossRefPubMed

12.

Laimer K, Spizzo G, Gastl G, et al. High EGFR expression predicts poor prognosis in patients with squamous cell carcinoma of the oral cavity and oropharynx: a TMA-based immunohistochemical analysis. Oral Oncol. 2007;43(2):193–8.CrossRefPubMed

13.

Herbst RS. Review of epidermal growth factor receptor biology. Int J Radiat Oncol Biol Phys. 2004;59:21–6.CrossRefPubMed

14.

Lin SY, Makino K, Xia W, et al. Nuclear localization of EGF receptor and its potential new role as a transcription factor. Nat Cell Biol. 2001;3(9):802–8.CrossRefPubMed

15.

Sah NK, Khan Z, Khan GJ, et al. Structural, functional and therapeutic biology of survivin. Cancer Lett. 2006;244(2):164–71.CrossRefPubMed

16.

Duffy MJ, O’Donovan N, Brennan DJ, et al. Survivin: a promising tumor biomarker. Cancer Lett. 2007;249:49–60.CrossRefPubMed

17.

Brennan DJ, Rexhepaj E, O’Brien SL, et al. Altered cytoplasmic-to-nuclear ratio of survivin is a prognostic indicator in breast cancer. Clin Cancer Res. 2008;14(9):2681–9.CrossRefPubMed

18.

Kumamoto H, Ooya K. Expression of survivin and X chromosome-linked inhibitor of apoptosis protein in ameloblastomas. Virchows Arch. 2004;444:164–70.CrossRefPubMed

19.

Andric M, Dozic B, Popovic B, et al. Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection. Oral Dis. 2010;16:156–9.CrossRefPubMed

20.

Saraçoğlu U, Kurt B, Günhan Ö, et al. MIB-1 expression in odontogenic epithelial rests, epithelium of health oral mucosa and epithelium of selected odontogenic cysts: an immunohistochemical study. Int J Oral Maxillofac Surg. 2005;34:432–5.CrossRefPubMed

21.

Lo Muzio L, Staibano S, Pannone G, et al. Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome. J Dent Res. 1999;78:1345–53.CrossRefPubMed

22.

De Paula AMB, Carvalhais JN, Domingues MG, et al. Cell proliferation markers in the odontogenic keratocyst: effect of inflammation. J Oral Pathol Med. 2000;29:477–82.CrossRefPubMed

23.

Kaplan I, Hirshberg A. The correlation between epithelial cell proliferation and inflammation in odontogenic keratocyst. Oral Oncol. 2004;40:985–91.CrossRefPubMed

24.

Damjanov I, Mildner B, Knowles BB. Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues. Lab Invest. 1986;55:588–92.PubMed

25.

Gulkesen KH, Kilicarslan B, Altunbas HA, et al. EGFR and p53 expression and proliferative activity in parathyroid adenomas: an immunohistochemical study. APMIS. 2001;109:870–4.CrossRefPubMed

26.

Lo Muzio L, Pannone G, Leonardi R, et al. Survivin, a potential early predictor of tumor progression in the oral mucosa. J Dent Res. 2003;82:923–8.CrossRefPubMed

27.

Lo Muzio L, Farina A, Rubini C, et al. Survivin as prognostic factor in squamous cell carcinoma of the oral cavity. Cancer Lett. 2005;225:27–33.CrossRefPubMed

28.

Tanaka C, Uzawa K, Shibahana T, et al. Expression of an inhibitor of apoptosis, Survivin, in oral carcinogenesis. J Dent Res. 2003;82:607–11.CrossRefPubMed

29.

Pizen J, Cör A, Gale N. Survivin expression is a negative marker in laryngeal squamous cell carcinoma and is associated with p53 accumulation. Histopathology. 2004;45:180–6.CrossRef

30.

Suzuki T, Kumanoto H, Kunimore K, et al. Immunohistochemical analysis of apoptosis-related factors in lining epithelium of radicular cysts. J Oral Pathol Med. 2005;34:46–52.CrossRefPubMed

31.

Thosaporn W, Iamaroon A, Pongsiriwets S, et al. A comparative study of epithelial cell proliferation between the odontogenic keratocysts, orthokeratinized odontogenic cyst and ameloblastoma. Oral Dis. 2004;10:22–6.CrossRefPubMed

32.

Oliveira MG, Lauxen IS, Chaves ACM, et al. Immunohistochemical analysis of the patterns of p53 and PCNA expression in odontogenic cystic lesions. Med Oral Patol Oral Cir Bucal. 2008;13(5):E275–80.PubMed

33.

Gurgel CAS, Ramos EAG, Azevedo RA, et al. Expression of Ki-67, p53 and p63 proteins in keratocyst odontogenic tumours: an immunohistochemistry study. J Mol Histol. 2008;39:311–6.CrossRefPubMed

34.

Gadbail AR, Chaudhary M, Patil S, et al. Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts. Oral Dis. 2009;15:490–8.CrossRefPubMed

35.

Piatelli A, Lezzi G, Fioroni M, et al. Ki-67 expression in dentigerous cysts, unicystic ameloblastoma and ameloblastomas arising from dental cysts. J Endod. 2002;28(2):55–8.CrossRef

36.

Fortugno P, Wall NR, Giodini A, et al. Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J Cell Sci. 2002;1151:575–85.

37.

De Maria S, Pannone G, Bufo P, et al. Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. J Cancer Res Clin Oncol. 2009;135(1):107–16.CrossRefPubMed

38.

Li F. Role of surviving and its splice variants in tumorigenesis. Br J Cancer. 2005;92(2):212–6.PubMed

Titel: Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors
verfasst von: Márcia Gaiger de Oliveira
Isabel da Silva Lauxen
Anna Cecília Moraes Chaves
Pantelis Varvaki Rados
Manoel Sant’Ana Filho
Publikationsdatum: 01.03.2011
Verlag: Springer US
Erschienen in: Head and Neck Pathology / Ausgabe 1/2011
Elektronische ISSN: 1936-0568
DOI: https://doi.org/10.1007/s12105-010-0216-0

Neu im Fachgebiet Pathologie

Open Access 15.04.2024 | Biomarker | Schwerpunkt: Next Generation Pathology

Springer Medizin

Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2011

Lymphangioma of the Vallecula

Not Your Usual Cancer Case: Variants of Laryngeal Squamous Cell Carcinoma

Oral Manifestations of Dermatologic Disease: A Focus on Lichenoid Lesions

Uncommon Foreign Body Reactions Occurring in the Lip: Clinical Misdiagnosis and the Use of Special Techniques of Analysis

Papillary Thyroid Carcinoma Variants

The Spectrum of Gnathic Osteosarcoma: Caveats for the Clinician and the Pathologist

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie