Introduction
Materials and methods
Clinical histories
Antibody (clone) | Source | Dilution | HIER |
---|---|---|---|
CD45 (LCA, 2B11/PD7/26) | DAKO Cytomation, Denmark A/S | 1:100 | LpH |
CD20 (L-26) | DAKO Cytomation, Denmark A/S | 1:300 | LpH |
CD3 (SP7, rabbit monoclonal) | LabVision Corporation, UK | 1:150 | LpH |
CD79a (SP-18, rabbit monoclonal) | LabVision Corporation, UK | 1:400 | T/E |
PAX-5 (24) | BD Transduction Laboratories™ | 1:40 | T/E |
CD10 (56C6) | Novocastra™ | 1:40 | T/E |
BCL-6 (PG-B6p) | DAKO Cytomation, Denmark A/S | 1:40 | T/E |
BCL-2 (Bcl-2/100/D5) | Novocastra™ | 1:20 | T/E |
CD21 (2G9) | Novocastra™ | 1:20 | T/E |
CD68 (PG-M1) | DAKO Cytomation, Denmark A/S | 1:250 | T/E |
CD14 (7) | Novocastra™ | 1:160 | T/E |
CD1a (MTB1) | Novocastra™ | 1:5 | T/E |
PU1 (polyclonal) | Santa Cruz Biotechnology | 1:300 | T/E |
MAF-B (goat polyclonal) | Santa Cruz Biotechnology | 1:2000 | T/E |
S-100 (polyclonal) | DAKO Cytomation, Denmark A/S | 1:1500 | LpH |
Ki67 (MIB-1) | DAKO Cytomation, Denmark A/S | 1:150 | T/E |
Electron microscopy
Cytogenetics
Results and discussion
Patient 1 | Patient 2 | ||||||
---|---|---|---|---|---|---|---|
Inguinal lymph node, 2001 | Inguinal lymph node, 2003 | Neck tumor, 2008 | Bone marrow, 2008 | Tongue, 2006 | Tongue, 2007 | Skin, 2007 | |
Morphology | Follicular lymphoma, grade 2 | Follicular lymphoma, grade 3 | Large atypical cells with large vesiculose nuclei and eosinophilic cytoplasm | Diffuse, intraparenchymatous infiltration by centroblasts | Diffuse large B cell lymphoma | Large atypical cells with large vesiculose nuclei and eosinophilic cytoplasm | Large atypical cells with large vesiculose nuclei and eosinophilic cytoplasm |
IHC | CD20+, CD79a+, PAX-5+, CD10+, BCL-6+, BCL-2+, PU1+, CD68−, CD14−, CD1a−, S100−, MAF-B- | CD20+, CD79a+, PAX-5+, CD10+, BCL-6+, BCL-2+, PU1+, CD68−, CD14−, CD1a−, S100-, MAF-B- | CD20−, CD79a−, PAX-5−, CD10−/+, BCL-6+, BCL-2−, PU1+, MAF-B+, CD68+, CD14+, CD1a−/+, S100+ | CD20+, CD79a+, PAX-5+, CD10+, BCL-6+, BCL-2+, PU1+,CD68−, CD14−, CD1a−, S100−, MAF-B- | CD20+,CD79a, PAX-5+, CD10+, BCL-6+, BCL-2−, PU1+, CD68−, CD14−, CD1a−, S100−,MAF-B− | CD20−, CD79a−, PAX-5−, CD10−, BCL−6+, BCL-2−, PU1+, CD68+,CD14+/−, CD1a−/+,S100+, MAF-B− | CD20−, PAX-5−, CD10−/+, BCL-6+, BCL-2−, PU1+, CD68+, CD14−/+, CD1a−/+,S100+, MAF-B− |
FISH | t(14;18)(18q21) | t(14;18)(18q21) | t(14;18)(18q21) | t(14;18)(18q21) | t(14;18)(18q21) | t(14;18)(18q21) | t(14;18)(18q21) |
PCR analysis | Not performed | Monoclonal IgH gene rearrangement, one peak 326/327 bp | Monoclonal IgH gene rearrangement, one peak 326/327 bp | Not performed | Monoclonal IgH gene rearrangement, one peak 118 bp | Not performed | Monoclonal IgH gene rearrangement, one peak 118 bp |
Sequencing | Intact structure of the gene | Intact structure of the gene | Intact structure of the gene | Not performed | Not performed | Intact structure of the gene | Not performed |
EM | Not performed | Not performed | Birbeck granules | Not performed | |||
Cytogenetic data | 48,XY, +2,add(13)(q33), +8,t(14;18)(q32;q21), +17[14]/46,XY[1] | 43-49,XY, +2,del(6)(q14), +8,t(14;18)(q32;q21), +17[cp8]/46,XY[7] | 47~51,XY,+X, add(1)(p36),add(6)(q27), del(6)(q23),del(9)(p22), +12,dup(13)(q21q31), t(14;18)(q32;q21)[cp5]/46,XY[7] | Not performed | Not performed | Not performed | Not performed |