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Neurotoxicity Research

Erschienen in:

01.08.2010

Mechanism of the Specific Neuronal Toxicity of a Type I Ribosome-Inactivating Protein, Trichosanthin

verfasst von: Ou Sha, David Tai-Wai Yew, Eric Yu-Pang Cho, Tzi-Bun Ng, Lin Yuan, Wing-Hang Kwong

Erschienen in: Neurotoxicity Research | Ausgabe 2/2010

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Abstract

The aim was to study the mechanism of neuronal toxicity, the cellular pathway, and the glial cell reactions induced by trichosanthin (TCS), a type I ribosome-inactivating protein (RIP). Ricin A chain (RTA) was included for comparison. TCS, RTA, and fluorescein isothiocyanate (FITC)-labeled TCS and RTA were separately injected into rat eyes. Saline or pure FITC was used as the control. Electron microscopy, confocal microscopy, and lectin and immunohistochemical staining were used to study the neurotoxic mechanism. TCS mainly induced apoptosis by causing degeneration of the mitochondria. TCS was able to enter the Müller and pigment cells. It caused a change in cell number of the following types of glial cells: a decrease in Müller cells, an increase in astrocytes, and little change in microglia. In contrast, RTA mainly induced necrosis and entered vascular endothelial cells. Astrocyte and microglia reactions were stronger in the RTA-treated retinas than those in the TCS-treated retinas. In conclusion, TCS appears to selectively enter and destroy Müller and pigment epithelia cells, which subsequently induce the death of photoreceptors. Degeneration of mitochondria is involved in the pathways of apoptosis of the photoreceptors caused by TCS. In sharp contrast, RTA can enter vascular endothelial cells and damage the vascular endothelium, resulting in retinitis and necrosis.

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Titel: Mechanism of the Specific Neuronal Toxicity of a Type I Ribosome-Inactivating Protein, Trichosanthin
verfasst von: Ou Sha
David Tai-Wai Yew
Eric Yu-Pang Cho
Tzi-Bun Ng
Lin Yuan
Wing-Hang Kwong
Publikationsdatum: 01.08.2010
Verlag: Springer-Verlag
Erschienen in: Neurotoxicity Research / Ausgabe 2/2010
Print ISSN: 1029-8428
Elektronische ISSN: 1476-3524
DOI: https://doi.org/10.1007/s12640-009-9122-1

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