Background
Methods
Study population
Case definition
Blood samples and cytokine detection by multiplex microbead immunoassay
Extracellular staining for flow cytometry analysis
Statistical analyses
Results
Clinical characterisation of dengue disease in adult Brazilian patients
Characteristics | Mild1
| Severe | Fisher's exact test | ||
---|---|---|---|---|---|
Age (median years, 25–75%)
2
| 39, 28–48 | 37, 23–53 | |||
Sex (M:F) (Patient Number) | 7:13 | 26:13 | |||
DENV-1: DENV-3 (Patient Number) | 20:0 | 8:31 | p < 0.0001 | ||
%
3
|
N
4
|
%
|
N
| ||
Fever |
30
|
20
|
8
|
37
| |
Haemorrhagic manifestations
5
|
35
|
20
|
41
|
37
| |
Petechia |
21
|
19
|
49
|
37
| |
Abdominal Pain |
19
|
16
|
33
|
36
| |
Thrombocytopenia (<100.000 counts/mm3) |
63
|
19
|
72
|
39
| |
Thrombocytopenia (<50.000 counts/mm3) |
0
|
19
|
46
|
39
| |
Hipotension
6
|
0
|
20
|
67
|
39
| |
Hemoconcentration
7
|
33
|
15
|
10
|
39
| |
Secondary Infection |
26
|
19
|
42
|
33
| P = 0.37 |
Circulating cytokines are elevated in dengue patients during early febrile phase increasing their levels during defervescence
Plasma cytokine levels are changed in patients with severe dengue and other clinical manifestations
Severity1 Yes = 39, No = 20 3
| Coefficient | Error | P value (significance)4
| OR2(CI 95% range) |
---|---|---|---|---|
Intercept
|
10.855
|
4.150
|
0.0089**
| - |
Log (MIP-1β +1e-4) -
|
1.712
|
0.705
|
0.0153*
|
0.181(0.045–0.72) |
Log (IFN-γ +1e-4)
|
0.130
|
0.046
|
0.0046**
|
1.138 (1.041–1.245) |
Platelet counts 50.000/mm
3
Yes = 18 No = 40 |
Coefficient
|
Error
|
P value (significance) |
OR (CI 95% range) |
Intercept
|
0.940
|
0.943
|
0.3187
| - |
MIP-1β
|
-0.010
|
0.023
|
0.0057**
|
0.990 (0.983–0.997) |
IL-1β
|
0.056
|
0.023
|
0.0123*
|
1.058 (1.012–1.106) |
TNF-α
|
-0.022
|
0.008
|
0.0100*
|
0.978(0.962–0.995) |
MCP-1
|
0.002
|
0.001
|
0.0437*
|
1.002 (1.0001–1.0036) |
Hypotension Yes = 24 No = 35 |
Coefficient
|
Error
|
P value (significance) |
OR (CI 95% range) |
Intercept
|
0.357
|
0.742
|
0.6302
| - |
IL-1β
|
0.075
|
0.038
|
0.0454*
|
0.928 (0.862–0.999) |
IL-12
|
0.005
|
0.012
|
0.6516
|
1.006 (0.982–1.030) |
IL-13
|
-0.013
|
0.014
|
0.3519
|
0.987 (0.960–1.015) |
MIP-1β
|
-0.004
|
0.002
|
0.0807
|
0.996 (0.992–1.000) |
MCP-1
|
0.001
|
0.0007
|
0.2065
|
1.001 (0.9995–1.002) |
GM-CSF
|
0.0043
|
0.0017
|
0.0214*
|
1.004 (1.001–1.007) |
MODELS1
| Coefficient | Standard Error | t value | P value (>| t|) (significance) |
---|---|---|---|---|
Platelet counts:
| ||||
(N = 59) | ||||
Intercept
| 81.42 | 18.9963 | 4.286 |
8.7e-05 ***
2
|
IL-1β | -0.180 | 0.1803 | -0.998 |
0.3233
|
IL-8
| -0.194 | 0.094 | -2.075 |
0.0434 *
|
IL-13
| 0.173 | 0.081 | 2.145 |
0.0371 *
|
MIP-1β | 0.130 | 0.049 | 2.630 |
0.0114 *
|
MCP-1
| -0.033 | 0.013 | -2.534 |
0.0146 *
|
Hematocrit:
| ||||
(N = 59) | ||||
Intercept
| 38.850 | 0.867 | 44.813 |
<2e-16 ***
|
Sex
| 4.850 | 1.043 | 4.649 |
2.55e-05 ***
|
IL-1β | 0.026 | 0.012 | 2.105 |
0.0405 *
|
IL-6
| -0.002 | 0.0007 | -3.074 |
0.0035 **
|
IL-12
| 0.011 | 0.004 | 2.492 |
0.0162 *
|