Background
Methods
Serum and tissue samples
Sandwich ELISA for serum CEACAM1
Immunohistochemical staining
Quantitative real-time polymerase chain reaction for the CEACAM1 RNA level
Primer name | Primer sequences | Product | |
---|---|---|---|
Real-time PCR
|
CEACAM1
*
|
F:5’-CAGTCACCTTGAATGTCACCTATG-3’
|
144 bp
|
R:5’-GTTCCATTGATAAGCCAGGAGTAC-3
| |||
GAPDH
|
F: 5’-GCACCGTCAAGGCTGAGAAC-3’
|
142 bp
| |
NM_002046.
|
R: 5’-ATGGTGGTGAAGACGCCAGT-3’
| ||
Reverse transcription-PCR
|
CEACAM1
*
|
F:5’-GGTTGCTCTGATAGCAGTAG-3’
|
408 bp (L-form)
|
R: 5’-AGCCTGGAGATGCCTATTAG-3’
|
355 bp (S-form)
| ||
GAPDH
|
F:5’-GGGAAGGTGAAGGTCGGAGTC-3’
|
570 bp
| |
NM_002046.
|
R:5’-AGGGGCCATCCACAGTCTTCT-3’
|
Reverse transcription-PCR for the expression patterns of CEACAM1 isoforms
Statistical analysis
Results
CEACAM1 serum levels
Groups | No. | CEACAM1 | ||
---|---|---|---|---|
Median (P50) | Range |
P value | ||
Age
| ||||
<60
|
16
|
550.70
|
381.30 ~ 735.84
|
0.679
|
≥60
|
19
|
535.32
|
442.07 ~ 968.13
| |
Sex
| ||||
Male
|
16
|
588.98
|
442.07 ~ 968.13
|
0.042*
|
Female
|
19
|
513.95
|
381.30 ~ 735.84
| |
Location
| ||||
Left
|
11
|
519.89
|
381.30 ~ 674.69
|
0.619
|
Right
|
24
|
548.34
|
442.07 ~ 968.13
| |
Staging**
| ||||
Stage Ia ~ IIb
|
19
|
566.04
|
381.03 ~ 968.13
|
0.016*
|
Stage IIIa ~ IV
|
16
|
499.66
|
442.07 ~ 638.68
| |
Grading
| ||||
G1 ~ 2
|
14
|
542.42
|
442.07 ~ 968.13
|
0.775
|
G3 ~ 4
|
21
|
544.79
|
381.30 ~ 735.84
| |
Histology
| ||||
Squamous cell carcinoma
|
6
|
571.31
|
499.66 ~ 627.02
|
0.180
|
Adenocarcinoma
|
19
|
519.89
|
381.30 ~ 735.84
| |
Others***
|
10
|
579.29
|
442.07 ~ 968.13
| |
Lymph node metastasis
| ||||
Node negative
|
13
|
566.04
|
381.30 ~ 968.13
|
0.274
|
Node positive
|
22
|
532.34
|
442.07 ~ 735.84
| |
Invasion depth
| ||||
pT1 ~ pT2
|
26
|
551.88
|
381.30 ~ 968.13
|
0.131
|
pT3 ~ pT4
|
9
|
519.89
|
442.07 ~ 638.68
|
CEACAM1 protein and mRNA expression in lung tissues
Groups | No. | Tumour tissue | Normal tissue | ||||
---|---|---|---|---|---|---|---|
Median (P50) | Range |
P value | Median (P50) | Range |
P value | ||
Age
| |||||||
≥60
|
13
|
0.0293
|
5.60 × 10
-4
~ 0.0716
|
0.082
|
5.31 × 10
-3
|
2.28 × 10
-3
~ 0.0172
|
0.828
|
<60
|
8
|
2.62 × 10
-3
|
9.25 × 10
-4
~ 0.0287
|
6.99 × 10
-3
|
3.80 × 10
-4
~ 0.0113
| ||
Sex
| |||||||
Male
|
12
|
3.09 × 10
-3
|
5.60 × 10
-4
~ 0.0373
|
0.028*
|
4.31 × 10
-3
|
3.80 × 10
-4
~ 0.0172
|
0.088
|
Female
|
9
|
0.0293
|
1.36 × 10
-3
~ 0.0716
|
8.67 × 10
-3
|
3.81 × 10
-3
~ 0.0114
| ||
Staging***
| |||||||
Stage Ia ~ IIb
|
14
|
0.0126
|
5.60 × 10
-4
~ 0.0693
|
0.332
|
5.61 × 10
-3
|
5.60 × 10
-4
~ 0.0693
|
0.709
|
Stage IIIa ~ IV
|
7
|
2.09 × 10
-3
|
9.24 × 10
-4
~ 0.716
|
7.87 × 10
-3
|
2.85 × 10
-3
~ 0.0113
| ||
Grading
| |||||||
G1 ~ 2
|
10
|
0.0126
|
1.04 × 10
-3
~ 0.716
|
0.439
|
8.60 × 10
-3
|
3.20 × 10
-3
~ 0.172
|
0.020*
|
G3 ~ 4
|
11
|
3.21 × 10
-3
|
5.60 × 10
-4
~ 0.422
|
3.81 × 10
-3
|
3.80 × 10
-4
~ 0.0113
| ||
Histology
| |||||||
Squamous
1
|
5
|
0.00144
|
9.246 × 10
-4
~ 0.0032
|
0.0032
|
3.80 × 10
-4
~ 0.0171
| ||
Adenocarcinoma
|
11
|
0.0286
|
0.0013 ~ 0.072
|
0.003*
|
0.00787
|
0.0034 ~ 0.0114
|
0.585
|
Others**
|
5
|
0.0021
|
5.60 × 10
-4
~ 0.014
|
0.00853
|
0.0023 ~ 0.011
| ||
Lymph node metastasis
| |||||||
node negative
|
7
|
0.0140
|
1.04 × 10
-3
~ 0.0658
|
0.502
|
8.53 × 10
-3
|
3.81 × 10
-3
~ 0.017
|
0.117
|
node positive
|
14
|
3.19 × 10
-3
|
5.60 × 10
-4
~ 0.0716
|
4.98 × 10
-3
|
3.80 × 10
-4
~ 0.011
| ||
Invasion depth
| |||||||
pT1 ~ pT2
|
17
|
0.0129
|
5.60 × 10
-4
~ 0.0716
|
0.039*
|
2.12 × 10
-3
|
3.80 × 10
-4
~ 0.0172
|
0.720
|
pT3 ~ pT4
|
4
|
1.63 × 10
-3
|
9.25 × 10
-4
~ 3.21 × 10
-3
|
6.28 × 10
-3
|
2.85 × 10
-3
~ 0.0113
|
CEACAM1 isoform expression patterns
Groups | Median | 95% CI |
P value |
---|---|---|---|
Tumour S-form (IOD) | 15.89 | 11.95 ~ 60.44 | 0.023* |
Normal S-form (IOD) | 9.16 | 7.23 ~ 16.45 | |
Tumour L-form (IOD) | 10.43 | 8.05 ~ 14.58 | 0.917 |
Normal L-form (IOD) | 10.45 | 7.92 ~ 13.60 | |
Tumour (S:L) ratio | 2.44 | 1.49 ~ 4.20 | 0.016* |
Normal (S:L) ratio | 0.96 | 0.86 ~ 1.28 |