Background
Methods
Cell lines
Cancer tissue microarray immunohistochemistry
Western blot
Transduction of dominant negative Stat3 Y705F in cancer cells
Treatment of STA-21 and cell viability assay
Caspases 3, 8, and 9 immuno-fluorescent staining and acridine orange staining
Results
Elevated Stat3 phosphorylation in rhabdomyosarcoma, osteosarcoma and other soft-tissue sarcoma tissues and cell lines
Clinicopathological parameters | Numbers (%) | |
---|---|---|
Gender (total 264) | ||
Female | 113 (42.7) | |
Male | 151 (57.2) | |
Age (years) (total 264) | 1–20 | 65 (24.6) |
21–40 | 98 (36.9) | |
41–60 | 55 (20.7) | |
61–80 | 42 (15.8) | |
81–100 | 4 (1.5) | |
Grade (total 15) | I | 14 (93.3)a
|
III | 1 (6.7)a
| |
Histology (total 264) | Osteosarcoma | 113 (42.5) |
Liposarcoma | 32 (12.1) | |
Chondrosarcoma | 28 (10.6) | |
Histiosarcoma | 26 (9.8) | |
Leiomyosarcoma | 19 (7.1) | |
Fibrosarcoma | 16 (6.0) | |
Neurilemmoma | 12 (4.4) | |
Neurofibroma | 4 (1.5) | |
Synovial Sarcoma | 4 (1.5) | |
Hemangiosarcoma | 3 (1.1) | |
Alveolar Sarcoma | 2 (0.75) | |
Adenocarcinoma | 1 (0.38) | |
Epithelioid hemangioendothelioma | 1 (0.38) | |
Malignant Glomus Thigh Tumor | 1 (0.38) | |
Malignant Schwannoma | 1 (0.38) | |
Malignant Tienosynovial Tumor | 1 (0.38) | |
Sacrococcygeal Melanoma | 1 (0.38) |
Variable | Number of patients (32 each type) | |
---|---|---|
ARMS | ERMS | |
Gender | ||
Male | 13 | 24 |
Female | 19 | 8 |
Age (years) | ||
0–4 | 11 | 18 |
5–14 | 14 | 10 |
15–19 | 7 | 4 |
Mean | 9.1 | 5.8 |
Median | 10 | 4 |
Stage | ||
I | 5 | 7 |
II | 5 | 4 |
III | 15 | 14 |
IV | 7 | 7 |
Primary site | ||
Head and Neck | 11 | 10 |
Extremity | 12 | 3 |
Genitourinary | 4 | 9 |
Others | 5 | 10 |