Introduction
Materials and methods
Animal care
Transverse aortic constriction
RNA isolation and real-time PCR
Forward primer | Reverse primer | Amplicon(bp) | |
---|---|---|---|
Glut1 | TCAACACGGCCTTCACTG | CACGATGCTCAGATAGGACATC | 164 |
Glut2 | TGTGCTGCTGGATAAATTCGCCTG | AACCATGAACCAAGGGATTGGACC | 109 |
Glut3 | TTCTGGTCGGAATGCTCTTC | AATGTCCTCGAAAGTCCTGC | 143 |
Glut4 | GTAACTTCATTGTCGGCATGG | AGCTGAGATCTGGTCAAACG | 155 |
Glut5 | GGCTCATCTTCCCCTTCATTC | ATGAATGTCCTGCCCTTGG | 145 |
Glut6 | TTGGTGCTGTGAGGCT | TGGCACAAACTGGACGTA | 140 |
Glut8 | TTCATGGCCTTTCTAGTGACC | GAGTCCTGCCTTTAGTCTCAG | 145 |
Glut9 | TGCTTCCTCGTCTTCGCCACAATA | CTCTTGGCAAATGCCTGGCTGATT | 113 |
Glut10 | ACCAAAGGACAGTCTTTAGCTG | ATCTTCCAAGCAGACGGATG | 148 |
Glut12 | GGGTGTCAACCTTCTCATCTC | CCAAAGAGCATCCCTTAGTCTC | 149 |
Actin | GATTACTGCTCTGGCTCCTAG | GACTCATCGTACTCCTGCTTG | 147 |
HPRT | CCCCAAAATGGTTAAGGTTGC | AACAAAGTCTGGCCTGTATCC | 76 |
Western blotting
Statistical analyses
Results
GLUT expression in left ventricle
GLUT mRNA levels in murine left ventricles
C57BL/6J males (n = 8) | |
---|---|
GLUT1 | 982 ± 139 |
GLUT2 | NA |
GLUT3 | 62 ± 8 |
GLUT4 | 8878 ± 1532 |
GLUT5 | NA |
GLUT6 | NA |
GLUT8 | 360 ± 67 |
GLUT9 | NA |
GLUT10 | 73 ± 26 |
GLUT12 | 44 ± 5 |
ACTIN | 15998 ± 2582 |
FVB/NJ males (n = 8) | |
---|---|
GLUT1 | 457 ± 75 |
GLUT2 | NA |
GLUT3 | 26 ± 2 |
GLUT4 | 3202 ± 447 |
GLUT5 | NA |
GLUT6 | 8 ± 2 |
GLUT8 | 268 ± 34 |
GLUT9 | NA |
GLUT10 | 18 ± 2 |
GLUT12 | 170 ± 22 |
ACTIN | 9020 ± 1212 |