Background
Methods
Animals
RNA extraction
Preparation of probes
Microarray hybridization, scanning, and analysis
Results
Validation of data
GD15.5 | GD16.5 | GD17.5 | ||||
---|---|---|---|---|---|---|
M | F | M | F | M | F | |
Detected probesets (% present)*
| 12955 (57,3%) | 12988 (57,4%) | 12660 (56%) | 12455 (55%) | 12234 (54,1%) | 12808 (56,6%) |
Known genes
†
| 6490 | 6464 | 6466 | 6424 | 6321 | 6561 |
Unknown transcripts
†
| 2406 | 2433 | 2030 | 2019 | 1965 | 2060 |
Id* | Accession number | Gene name† | Fold changes‡ | ||||
---|---|---|---|---|---|---|---|
Symbol | GD15.5 (62)§ | GD16.5 (33) | GD17.5 (38) | Chr. | |||
Apoptosis
| |||||||
#1 | BI662863 | Rho-associated coiled-coil forming kinase 1 | Rock1 | 2.25 | |||
#2 | NM_011997 | caspase 8 associated protein 2 | Casp8ap2 | 2.15 | 4 11.4 cM | ||
Cell adhesion
| |||||||
#3 | NM_009925 | procollagen, type X, alpha 1 | Col10a1 | M | 10 22.0 cM | ||
#4 | L20232 | integrin binding sialoprotein | Ibsp | M | 5 56.0 cM | ||
#5 | NM_031163 | procollagen, type II, alpha 1 | Col2a1 | 2.96 | F | 15 54.5 cM | |
#6 | NM_007729 | procollagen, type XI, alpha 1 | Col11a1 | 2.25 | 3 53.1 cM | ||
#7 | AK004383 | procollagen, type IX, alpha 1 | Col9a1 | 1.96 | 1 15.0 cM | ||
#8 | U08020 | procollagen, type I, alpha 1 | Col1a1 | (1.77) | 11 56.0 cM | ||
Cell growth
| |||||||
#9 | NM_008341 | insulin-like growth factor binding protein 1 | Igfbp1 | M | 11 1.3 cM | ||
Coagulation
| |||||||
#10 | NM_008877 | plasminogen | Plg | F | F | 17 7.3 cM | |
#11 | AK011118 | fibrinogen, B beta polypeptide | Fgb | F | F | 3 48.2 cM | |
#12 | NM_133862 | fibrinogen, gamma polypeptide | Fgg | (3.81) | F | 3 41.3 cM | |
#13 | BC005467 | fibrinogen, alpha polypeptide | Fga | (3.10) | F | 3 44.8 cM | |
#14 | NM_010168 | coagulation factor II | F2 | (2.67) | 2 47.5 cM | ||
Endopeptidase activity
| |||||||
#15 | BC012874 | serine (or cysteine) proteinase inhibitor, clade A, member 1a | Serpina1a | F | F | 12 51.0 cM | |
#16 | NM_007443 | alpha 1 microglobulin/bikunin | Ambp | F | F | 4 30.6 cM | |
#17 | NM_010582 | inter-alpha trypsin inhibitor, heavy chain 2 | Itih2 | F | F | 2 1.0 cM | |
#18 | NM_008407 | inter-alpha trypsin inhibitor, heavy chain 3 | Itih3 | F | F | ||
#29 | NM_007618 | serine (or cysteine) proteinase inhibitor, clade A, member 6 | Serpina6 | (2.66) | F | 12 51.0 cM | |
#20 | NM_019429 | protease, serine, 16 (thymus) | Prss16 | (2.65) | 13 10.0 cM | ||
Erythroid-associated
| |||||||
#21 | NM_013848 | erythroblast membrane-associated protein | Ermap | F | 4 57.0 cM | ||
#22 | AF069311 | Rhesus blood group CE and D | Rhced | F | 4 65.7 cM | ||
#23 | NM_053149 | hemogen | Hemgn | (3.29) | F | F | |
#24 | NM_010635 | Kruppel-like factor 1 (erythroid) | Klf1 | (3.01) | |||
#25 | NM_133245 | erythroid associated factor | Eraf | (2.38) | |||
#26 | NM_010369 | glycophorin A | Gypa | (2.27) | 8 36.0 cM | ||
#27 | AJ007909 | erythroid differentiation regulator 1 | Erdr1 | (1.75) | |||
Hormone metabolism/regulation
| |||||||
#28 | NM_008293 | hydroxysteroid dehydrogenase-1, delta<5>-3-beta | Hsd3b1 | M | 3 49.1 cM | ||
#29 | AV021656 | aldo-keto reductase family 1, member B7 | Akr1b7 | M | 6 14.0 cM | ||
#30 | NM_009995 | cytochrome P450, family 21, subfamily a, polypeptide 1 | Cyp21a1 | M | |||
#31 | AI195150 | group specific component | Gc | F | F | 5 44.0 cM | |
#32 | BG141874 | transthyretin | Ttr | (3.93) | F | F | 18 7.0 cM |
#33 | NM_031192 | renin 1 structural | Ren1 | 3.67 | F | ||
#34 | NM_007423 | alpha fetoprotein | Afp | (2.64) | F | 5 50.0 cM | |
#35 | U63146 | retinol binding protein 4, plasma | Rbp4 | (1.89) | (2.16) | 19 38.0 cM | |
Immune functions
| |||||||
#36 | NM_011558 | T-cell receptor gamma, variable 4 | Tcrg-V4 | F | F | F | |
#37 | NM_009019 | recombination activating gene 1 | Rag1 | F | F | 2 56.0 cM | |
#38 | M58149 | CD3 antigen, gamma polypeptide | Cd3g | F | F | 9 26.0 cM | |
#39 | AF119253 | histocompatibility 2, class II antigen A, alpha | H2-Aa | F | |||
#40 | AV058500 | P lysozyme structural | Lzp-s | 3.42 | 1.70 | ||
#41 | X67128 | T-cell receptor beta, variable 13 | Tcrb-V13 | (1.97) | F | F | 6 20.5 cM |
#42 | BC011474 | lymphocyte protein tyrosine kinase | Lck | (2.87) | 4 59.0 cM | ||
#43 | BC003476 | Ia-associated invariant chain | Ii | (2.10) | 18 32.0 cM | ||
Lipid metabolism
| |||||||
#44 | NM_013475 | apolipoprotein H | Apoh | F | F | 11 63.0 cM | |
#45 | NM_009692 | apolipoprotein A-I | Apoa1 | F | F | 9 27.0 cM | |
#46 | NM_009695 | apolipoprotein C-II | Apoc2 | (3.20) | F | 7 4.0 cM | |
#47 | NM_013474 | apolipoprotein A-II | Apoa2 | (2.27) | F | 1 92.6 cM | |
#48 | BC002148 | fatty acid binding protein 4, adipocyte | Fabp4 | 2.33 | 3 13.9 cM | ||
#49 | BG922397 | p47 protein | p47 | 1.84 | |||
Protein biosynthesis
| |||||||
#50 | NM_012011 | eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked | Eif2s3y | M | M | M | |
#51 | NM_009079 | ribosomal protein L22 | Rpl22 | 1.86 | |||
#52 | AI642440 | ribosomal protein S13 | Rps13 | (1.81) | |||
Signal transduction
| |||||||
#53 | X67702 | secretoglobin, family 1A, member 1 (uteroglobin) | Scgb1a1 | M | |||
#54 | BF537798 | receptor (calcitonin) activity modifying protein 2 | Ramp2 | 2.07 | 2.16 | 11 61.5 cM | |
#55 | NM_016721 | IQ motif containing GTPase activating protein 1 | Iqgap1 | 1.96 | 7 39.0 cM | ||
Transcriptionnal regulation
| |||||||
#56 | AA210261 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked | Ddx3y | M | M | M | Y 2.07 cM |
#57 | AF127244 | jumonji, AT rich interactive domain 1D (Rbp2 like) | Jarid1d | M | M | M | Y 2.03 cM |
#58 | AA617392 | Max protein | Max | F | 12 32.0 cM | ||
#59 | BB393998 | flap structure specific endonuclease 1 | Fen1 | 3.70 | (2.96) | ||
#60 | NM_007496 | AT motif binding factor 1 | Atbf1 | (2.16) | 8 E1 | ||
Transport
| |||||||
#61 | BC024643 | albumin 1 | Alb1 | (2.71) | (8.68) | 5 50.0 cM | |
#62 | AF440692 | Transferring | Trf | (2.02) | (3.29) | 9 56.0 cM | |
#63 | BB448377 | solute carrier family 4 (anion exchanger), member 1 | Slc4a1 | (2.07) | 11 62.0 cM | ||
#64 | NM_026331 | mitochondrial solute carrier protein | Mscp | (1.90) | |||
Others
| |||||||
#65 | BB742957 | ubiquitously transcribed tetratricopeptide repeat gene, Y chr. | Uty | M | M | M | Y 2.06 cM |
#66 | AI256465 | alpha-2-HS-glycoprotein | Ahsg | F | F | 16 15.0 cM | |
#67 | AK003182 | myosin, light polypeptide 1 | Myl1 | F | 1 34.1 cM | ||
#68 | BG806300 | inactive X specific transcripts | Xist | (3.34) | (13.22) | (2.41) | X 42.0 cM |
#69 | AV224521 | Gelsolin | Gsn | 3.27 | 2 24.5 cM | ||
#70 | NM_025711 | Asporin | Aspn | 2.72 | |||
#71 | BC002136 | coronin, actin binding protein 1A | Coro1a | (2.48) | 7 62.5 cM | ||
#72 | AK017440 | RNA imprinted and accumulated in nucleus | Rian | (2.27) | 12 54.5 cM | ||
#73 | AV156640 | expressed in non-metastatic cells 1, protein | Nme1 | 2.23 | |||
#74 | AF291655 | Tenomodulin | Tnmd | 2.12 | |||
#75 | BB745314 | male-specific lethal-2 homolog (Drosophila) | Msl2 | (2.11) | |||
#76 | BB490338 | calponin 3, acidic | Cnn3 | 2.04 | |||
#77 | BB475271 | LUC7-like 2 (S. cerevisiae) | Luc7l2 | 1.96 | |||
#78 | NM_053123 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 | Smarca1 | 1.93 | |||
#79 | BM207360 | unc-50 homolog (C. elegans) | Unc50 | 1.91 | |||
#80 | AV011848 | malate dehydrogenase 1, NAD (soluble) | Mdh1 | 1.89 | 11 12.0 cM | ||
#81 | AA270173 | Lamin B1 | Lmnb1 | (1.89) | 18 29.0 cM | ||
#82 | BB192717 | protein phosphatase 2 (formerly 2A), reg. subunit A (PR 65), alpha isoform | Ppp2r1a | (1.88) | |||
#83 | BE372352 | ARP3 actin-related protein 3 homolog (yeast) | Actr3 | 1.77 | |||
Unknowns
| |||||||
#84 | AA717264 | Transcribed sequences | 2.51 | ||||
#85 | BC021831 | CDNA clone MGC:67258 IMAGE:6413648, complete cds | (2.14) | (2.36) | |||
#86 | AK018316 | DNA segment, Chr 2, ERATO Doi 145, expressed | D2Ertd145e | 2.31 | 2 31.0 cM | ||
#87 | BE865094 | expressed sequence AI448196 | AI448196 | (2.14) | |||
#88 | BB151477 | RIKEN cDNA 2810468K05 gene | 2810468K05Rik | 1.88 |
Several genes of interest exhibiting no sex difference in expression
FGF signaling pathway | TGF-beta signaling pathway | Surfactant associated proteins | Others | |
---|---|---|---|---|
Spry | Fgfbp1 | Tgfb1 | Sftpa | Egfr |
Fgf1 | Fgfr1op2 | Tgfb2 | Sftpb | Igf1 |
Fgf7 | Fgfrap1 | Tgfb3 | Sftpc | |
Fgf13 | Akr1b8 | Tgfbr1 | Sftpd | |
Fgf18 | Fgfrl1 | Tgfbr2 | ||
Fgfr1 | Tgfbr3 | |||
Fgfr2 | ||||
Fgfr3 | ||||
Fgfr4 |