Background
Methods
Tissue collection
Endogenous production of H2S
Western blotting
Statistical analysis
Results
Assay validation
Basal production of H2S in rat liver, rat intrauterine tissues and human placenta
Tissue | Treatment | H2S production rate (nM/min/g) | Significance | ||
---|---|---|---|---|---|
Mean | SD | n | |||
Rat liver | Control | 4.0 | 3.5 | 4 | |
L-cysteine | 777 | 163 | 4 | P < 0.05 | |
Rat uterus | Control | 0 | 0 | 4 | |
L-cysteine | 168 | 100 | 4 | P < 0.05 | |
Rat placenta | Control | 0 | 0 | 4 | |
L-cysteine | 11.1 | 4.7 | 4 | P < 0.05 | |
Rat fetal membranes | Control | 0 | 0 | 4 | |
L-cysteine | 22.3 | 15.0 | 4 | P < 0.05 | |
Human placenta | Control | 0 | 0 | 4 | |
L-cysteine | 200 | 102 | 4 | P < 0.05 |
Endogenous production of H2S in the presence of a nitric oxide donor
Tissue | Treatment | H2S production rate (nM/min/g) | Significance | ||
---|---|---|---|---|---|
Mean | SD | n | |||
Rat liver | -ve control | 4.0 | 3.5 | 4 | |
L-cysteine | 575 | 172 | 4 | ||
+ NO donor | 596 | 174 | 4 | NS | |
Rat uterus | -ve control | 0 | 0 | 4 | |
L-cysteine | 438 | 277 | 4 | ||
+ NO donor | 433 | 224 | 4 | NS | |
Rat placenta | -ve control | 0 | 0 | 4 | |
L-cysteine | 45.7 | 51.6 | 4 | ||
+ NO donor | 51.6 | 26.2 | 4 | NS | |
Rat fetal membranes | -ve control | 0 | 0 | 4 | |
L-cysteine | 43.4 | 20.3 | 4 | ||
+ NO donor | 57.4 | 31.5 | 4 | P < 0.05 |
Endogenous production of H2S under low oxygen conditions
Tissue | Treatment | H2S production rate (nM/min/g) | Significance | ||
---|---|---|---|---|---|
Mean | SD | n | |||
Rat liver | -ve control | 4.0 | 3.5 | 4 | |
L-cysteine | 331 | 269 | 4 | ||
Low O2 | 454 | 205 | 4 | P < 0.05 | |
Rat uterus | -ve control | 0 | 0 | 4 | |
L-cysteine | 405 | 432 | 4 | ||
Low O2 | 606 | 620 | 4 | P < 0.05 | |
Rat placenta | -ve control | 0 | 0 | 4 | |
L-cysteine | 20 | 10.9 | 4 | ||
Low O2 | 17.1 | 11.2 | 4 | NS | |
Rat fetal membranes | -ve control | 0 | 0 | 4 | |
L-cysteine | 146 | 120 | 4 | ||
Low O2 | 490 | 218 | 4 | P < 0.05 | |
Human placenta | -ve control | 0 | 0 | 4 | |
L-cysteine | 200 | 102 | 4 | ||
Low O2 | 397 | 326 | 4 | P < 0.05 |