Vitamin D deficiency and prognostics among patients with pancreatic adenocarcinoma

Pancreatic cancer is the fourth leading cause of cancer-related death in the United States. In 2013, an estimated 45,220 new cases of pancreatic adenocarcinoma were diagnosed, and nearly 38,460 people died as a result of the disease [1]. Surgery is the only curative measure, but more than half of patients are not candidates for surgical resection at the time of diagnosis, and the 5-year survival rate is just 6% [1].

Vitamin D is a steroid hormone that plays an essential role in the maintenance of calcium, phosphate, and bone metabolism. Emerging evidence demonstrates that vitamin D has a plethora of antitumor properties as well, including the induction of cell differentiation, the stimulation of apoptosis, and the inhibition of cell proliferation, angiogenesis, and metastasis [2, 3]. Ample epidemiologic studies have suggested that individuals with sufficient vitamin D levels are at lower risk for multiple types of cancers, including colorectal, breast, prostate, lung, and ovarian cancers [4]. For example, the risk of developing colorectal cancer was 50% lower among individuals with serum 25-hydroxyvitamin D (25[OH]D) levels of 33 ng/mL or more as compared with those with 25(OH)D levels of 12 ng/mL or less [5]. The risk of pancreatic cancer in association with vitamin D levels has been controversial; one study suggested an increased risk of cancer with 25(OH)D levels of more than 100 nmol/L [6], whereas another reported that the plasma level of 25(OH)D was inversely associated with the odds of developing pancreatic cancer [7]. A recent Canadian study suggested that the genes involved in the vitamin D pathway are associated with pancreatic cancer risk [8].

Vitamin D levels have also been shown to have an impact on the clinical outcomes of several cancer types, including breast, colon, lung, and prostate cancers as well as leukemia and lymphoma [9‐13]. For example, higher 25(OH)D levels at the time of diagnosis in patients with colorectal cancer were associated with a significant reduction in overall mortality (p = 0.02) and an improvement in overall survival [10]. Patients with prostate cancer whose serum 25(OH)D levels were medium (50–80 nmol/L) or high (>80 nmol/L) had significantly better prognoses as compared with those with low (<50 nmol/L) serum concentrations [11]. Although studies have suggested that higher vitamin D levels are significantly associated with improved survival among patients with certain cancers, to date no study has examined the relationship of vitamin D with pancreatic cancer survival.

We hypothesized that there is a high incidence of vitamin D deficiency in the pancreatic cancer patient population, because these patients frequently have exocrine dysfunction. Furthermore, we hypothesized that low vitamin D levels may be associated with worse prognoses in this patient population as has been seen with other malignancies. In this article, we report our study of the prevalence of vitamin D deficiency among patients with pancreatic adenocarcinoma, and we evaluate the prognostic significance of vitamin D levels for this patient population.

We conducted a retrospective review of patients with pancreatic adenocarcinoma who were evaluated at Siteman Cancer Center (SCC) in St. Louis, Missouri, between December 2007 and June 2011. The retrospective registry was established to capture all patients after institutional review board approval was obtained. Patients with pancreatic adenocarcinoma who had at least one vitamin D level recorded at the time of diagnosis or the first time that they presented to the center were included in the study. Patients were only excluded if they did not have their vitamin D levels checked at their first presentation. Patients with stage I and II pancreatic cancer usually presented to the medical oncologists after surgery. Therefore, their first recorded vitamin D levels were after their surgeries and before their first chemotherapy treatments. The same chemiluminescent assays were used to measure patient vitamin D levels at this center throughout the study.

Patients’ demographic information, clinical stage at the time of vitamin D assessment, vitamin D levels, and clinical outcomes were collected directly from the institutional electronic medical record system. Vitamin D levels at the initial clinical visit and at later visits after supplementation were recorded. Chemotherapy treatments were given at the discretion of oncologists. Data regarding the treatments that patients received were not collected. Patients’ times of death were obtained from the electronic medical record and verified by the Social Security Death Index. In accordance with previous studies, vitamin D deficiency was defined as a serum 25(OH)D level of less than 20 ng/mL; vitamin D insufficiency was defined as a 25(OH)D level of 20 ng/mL to 30 ng/mL; and vitamin D sufficiency was defined as a 25(OH)D level of at least 30 ng/mL [3, 14]. Patients’ vitamin D levels at the time of initial visit to SCC were used for prognostic studies.

Epidemiological studies have suggested a correlation between vitamin D status and an individual’s risk for pancreatic cancer [7]. There is a high prevalence of vitamin D deficiency in the general population (41.6%) [15] and among patients with other malignancies [16]. In our single-institution study, the prevalence of vitamin D deficiency and insufficiency was higher than that observed in the general population and occurred in 74% of the 178 patients studied. Klapdor and colleagues reported an even higher rate of 92.4% of 103 patients with pancreatic cancer who had 25(OH)D levels of less than 30 ng/mL [17]. More than 90% of our non-white pancreatic cancer patients had vitamin D deficiencies; this was consistent with the findings of other studies that also reported a high prevalence of vitamin D deficiency among non-whites.

To our knowledge, none of the prior studies assessed the correlation between pancreatic cancer stage and vitamin D status. Surprisingly, our study demonstrated that patients with stage I and II disease had lower vitamin D levels than patients with stage III and IV disease. Most of the patients with stage I and II disease were referred to medical oncologists after their surgeries; thus, their vitamin D levels were first checked after their operations. Among patients undergoing pancreatectomy, vitamin D levels are thought to be lower as a result of the surgical procedure itself in addition to associated exocrine dysfunction [18]. This idea is in alignment with the reported high rate of vitamin D deficiency found among patients after resection. The season in which the vitamin D level was obtained did not have a statistical impact, although higher levels were observed during summer and autumn. Although some studies have stated that patients with higher BMIs have lower vitamin D levels [19, 20], this was not observed in the current study. Patients with pancreatic cancer have lower vitamin D levels, regardless of the season or their BMI. Malabsorption and disease burden have more of an impact on vitamin D levels in these patients than synthetic insufficiency from the sunlight or storage capacities.

Our study further evaluated the association between baseline vitamin D levels and the prognoses of patients with pancreatic cancer. The association of the vitamin D level with the prognoses of various cancer populations has been variable in the literature. Although a sufficient vitamin D level was associated with a better prognosis in patients with colon cancer [10, 21] and a lower disease relapse and death rate in patients with melanoma [22], the prognostic value of the vitamin D level was not statistically significant in patients with non–small-cell lung cancer [23], and it was controversial in patients with breast cancer [24, 25]. We did not find an association between vitamin D deficiency and pancreatic cancer prognosis when all stages were combined. However, to our knowledge, this study is the first to suggest a significantly worse overall survival rate for patients with vitamin D levels of less than 20 ng/mL with stage III or IV pancreatic cancer. Multivariate analysis also confirms that the vitamin D level has an impact on the prognosis of pancreatic cancer. Although patients with stage I and II pancreatic cancer have lower vitamin D levels than those with stage III and IV, there is no significant association with poorer outcomes. The prognostic significance of vitamin D levels in patients with stage III and IV pancreatic cancer should be further examined in a prospective study.

The expression of the vitamin D receptor (VDR) and its polymorphisms modulate the activity of vitamin D, which may in turn affect the prognosis of patients with pancreatic cancer. Certain VDR polymorphisms show prognostic significance for melanoma, squamous cell cancer of the head and neck, and non–small-cell lung cancer [23, 26]. In a recent genome-wide association study of the overall survival of patients with pancreatic cancer, VDR gene polymorphism was associated with several prognostic factors [27]. Because patients with pancreatic cancer have a high prevalence of vitamin D deficiency in general, patients with lower VDR expression would have poorer prognoses as well as poorer responses to chemotherapy as a result of their vitamin D deficiencies. Our study was retrospective in nature and, unfortunately, patient VDR levels are not routinely checked, so we could not include VDR levels in our analysis. However, the future prospective examination of VDR levels in patients with pancreatic cancer is definitely warranted.

Our study suggested that the routine vitamin D supplementation of 50,000 International Units weekly for a short period of time (i.e., 10–12 weeks) may not be adequate to normalize vitamin D levels in most patients with pancreatic cancer. More prolonged supplementation may be required to achieve adequate levels. Exocrine pancreatic insufficiency may add another layer of complexity, thereby limiting sufficient supplementation with standard dosing. One study used a higher dose of vitamin D supplementation of up to 20,000 International Units daily for individuals with severe pancreatic exocrine insufficiency [17] and reported the normalization of vitamin D levels by individually adjusting oral vitamin D intake. The exact dose of supplementation may vary with the degree of insufficiency and the potential correction of the insufficiency with pancreatic enzymes. Regardless, the clinical impact of the normalization of vitamin D levels on disease progression and patient survival is still unknown and will require future prospective trials.

The current study is limited by its retrospective nature, the lack of accurate documentation of vitamin D supplementation before the diagnosis of pancreatic cancer, and the lack of control over vitamin D supplementation regimens. This was a single-institution study, so our cohort may differ from other pancreatic cancer populations. The study also demonstrates selection bias in that sicker patients were probably referred to our tertiary cancer center. The study attempted to not further limit the pool of patients by allowing all patients with baseline vitamin D level measurements to be included. Systemic treatment of patients was not controlled in this study but that would not be expected to bias the results in a particular way.

In summary, the current report confirms the high prevalence of vitamin D deficiency and insufficiency among patients with pancreatic cancer. Patients with early-stage pancreatic cancer have lower vitamin D levels as compared with those patients with advanced disease. However, only in patients with stage III and IV pancreatic cancer was there a significant association with poorer outcomes. We recommend the systematic screening of patients with pancreatic cancer for vitamin D deficiency and the rigorous monitoring of these patients while they are receiving oral supplementation. Further prospective investigations should be conducted to study vitamin D supplementation for patients with pancreatic cancer and to determine the prognostic value of vitamin D levels and VDR expression in this patient population.