Introduction
Materials and methods
Antibody (clone) | Manufacturer | Dilution | Pretreatment | Cutoff values |
---|---|---|---|---|
Maspin (EAW24) | Novocastra | 1:200 | Microwave | ≧10% (positive) |
CK5/6 (D5/16B4) | Dako | 1:50 | Water bath | ≧10% (positive) |
CK14 (LL002) | Novocastra | 1:100 | Water bath | ≧10% (positive) |
EGFR (EGFR.113) | Novocastra | 1:25 | Microwave | ≧10% (positive) |
CD10 (56C6) | Novocastra | 1:50 | Water bath | ≧10% (positive) |
p63 (4A4+Y4A3) | LabVision | 1:200 | None | ≧10% (positive) |
ER (1D5) | Dako | 1:50 | Water bath | ≧10% (positive) |
PgR (PgR636) | Dako | 1:800 | Water bath | ≧10% (positive) |
HER2 | Dako | Prediluted (Hercep test) | Water bath | Score 3+ (positive) |
Results
Parameters | Number | Percentage |
---|---|---|
Age (years) | ||
≤50 | 51 | 37.8 |
>50 | 84 | 62.2 |
Tumor size (mm) | ||
≤20 | 44 | 32.6 |
>20 | 91 | 67.4 |
Lymph node metastases | ||
0 | 82 | 60.7 |
1-3 | 28 | 20.7 |
3< | 24 | 17.8 |
Histological grade | ||
I | 5 | 3.7 |
II | 29 | 21.5 |
III | 101 | 74.8 |
Maspin | ||
Positive | 116 | 85.9 |
Negative | 19 | 14.1 |
EGFR | ||
Positive | 66 | 48.9 |
Negative | 69 | 51.1 |
CK5/6 | ||
Positive | 80 | 59.3 |
Negative | 55 | 40.7 |
CK14 | ||
Positive | 46 | 34.1 |
Negative | 89 | 65.9 |
CD10 | ||
Positive | 24 | 17.8 |
Negative | 111 | 82.2 |
p63 | ||
Positive | 17 | 12.6 |
Negative | 118 | 87.4 |
Maspin expression | |||
---|---|---|---|
Positive (85.9%) 116 cases | Negative (14.1%) 19 cases | P -value | |
Age (years) | |||
≤50 | 50 | 1 | 0.0015 |
>50 | 66 | 18 | |
Tumor size (mm) | |||
≤20 | 41 | 3 | 0.091 |
>20 | 75 | 16 | |
Lymph node metastases | |||
0 | 69 | 13 | 0.226 |
1-3 | 27 | 1 | |
3< | 20 | 4 | |
Histological grade | |||
I | 4 | 1 | 0.0013 |
II | 19 | 10 | |
III | 93 | 8 | |
EGFR | |||
Positive | 57 | 9 | 0.886 |
Negative | 59 | 10 | |
CK5/6 | |||
Positive | 77 | 3 | <0.0001 |
Negative | 39 | 16 | |
CK14 | |||
Positive | 45 | 1 | 0.0034 |
Negative | 71 | 18 | |
CD10 | |||
Positive | 19 | 5 | 0.293 |
Negative | 97 | 14 | |
p63 | |||
Positive | 15 | 2 | 0.769 |
Negative | 101 | 17 |
Maspin expression | |||
---|---|---|---|
Positive (85.9%) 116 cases | Negative (14.1%) 19 cases | P -value | |
CK5/6 and/or EGFR | |||
Positive | 92 | 11 | 0.041 |
Negative | 24 | 8 | |
CK5/6 and/or EGFR and/or CK14 | |||
Positive | 96 | 11 | 0.013 |
Negative | 20 | 8 |