Introduction
Materials and methods
Patients and tissue samples
Clinicopathological features | No. of cases | miR-224 expression | P | |
---|---|---|---|---|
High (n, %) | Low (n, %) | |||
Age
| ||||
<50 | 60 | 30 (50.0) | 30 (50.0) | NS |
≥50 | 66 | 36 (54.5) | 30 (45.5) | |
Tumor size (cm)
| ||||
<4.0 | 58 | 30 (51.7) | 28 (48.3) | NS |
≥4.0 | 68 | 36 (52.9) | 32 (47.1) | |
Histological grades
| ||||
Well differentiated | 28 | 4 (14.3) | 24 (85.7) | 0.03 |
Moderately differentiated | 32 | 12 (37.5) | 20 (62.5) | |
Poorly differentiated | 66 | 50 (75.8) | 16 (24.2) | |
FIGO stage
| ||||
Ib~IIa | 64 | 20 (31.3) | 44 (68.7) | 0.02 |
IIb~IIIa | 62 | 46 (74.2) | 16 (25.8) | |
Lymph node metastasis
| ||||
No | 79 | 24 (30.4) | 55 (69.6) | 0.008 |
Yes | 47 | 42 (89.4) | 5 (10.6) | |
Vascular invasion
| ||||
No | 72 | 20 (27.8) | 52 (72.2) | 0.01 |
Yes | 54 | 46 (85.2) | 8 (14.8) | |
HPV
| ||||
(−) | 48 | 10 (20.8) | 38 (79.2) | 0.02 |
(+) | 78 | 56 (71.8) | 22 (28.2) |
Real-time quantitative RT-PCR for miRNA
Statistical analysis
Results
MiR-224 upregulation in human cervical cancer
MiR-224 upregulation associates with aggressive clinicopatholigcal parameters of human cervical cancer
MiR-224 upregulation associates with poor prognosis in patients with human cervical cancer
Variables | Univariate log-rank test (P) | Cox multivariable analysis (P) | Relative risk (RR) |
---|---|---|---|
Age at diagnosis (years)
| |||
<50 vs. ≥50 | 0.3 | 0.7 | 0.6 |
Tumor size (cm)
| |||
<4.0 vs. ≥4.0 | 0.6 | 0.8 | 0.6 |
FIGO stage
| |||
Ib~IIa vs. IIb~IIIa | <0.001 | 0.01 | 6.2 |
Histological grades
| |||
Well ~ moderately differentiated vs. poor differentiated | 0.08 | 0.10 | 1.3 |
Lymph node metastasis
| |||
No vs. Yes | 0.006 | 0.02 | 3.9 |
Vascular invasion
| |||
No vs. Yes | 0.01 | 0.04 | 2.7 |
HPV
| |||
(−) vs (+) | 0.06 | 0.1 | 1.1 |
MiR-224 expression
| |||
High vs Low | <0.001 | 0.009 | 6.8 |