Background
Methods
Experimental design
Histomorphometry of the femur
Three-point bending test of the femur
Statistical analysis
Results
Genistein treatment decreased body mass
Lean STD | Lean + GEN | Obese STD | Obese + GEN | Genotype effect (P) | Treatment effect (P) | Genotype * treatment interaction (P) | |
---|---|---|---|---|---|---|---|
Starting body mass (g) | 22.1 ± 0.92 | 21.7 ± 0.53 | 37.0 ± 1.86 | 36.54 ± 1.11 | 0.01 | 0.73 | 0.99 |
Final body mass (g) | 24.8 ± 0.92 | 23.4 ± 0.46 | 50.7 ± 2.28 | 43.7 ± 1.29 | 0.01 | 0.01 | 0.06 |
PE Bone area (B.Ar.; mm2) | 0.79 ± 0.15 | 0.50 ± 0.07 | 0.85 ± 0.06 | 0.84 ± 0.04 | 0.04 | 0.11 | 0.12 |
PE Total area (Tt.Ar.; mm2) | 1.46 ± 0.26 | 0.84 ± 0.13 | 1.64 ± 0.17 | 1.44 ± 0.09 | 0.04 | 0.03 | 0.25 |
MS Cortical area (Ct.Ar.; mm2) | 1.15 ± 0.06 | 1.20 ± 0.06 | 1.17 ± 0.03 | 1.08 ± 0.07 | 0.36 | 0.70 | 0.22 |
MS IMAX (mm4) | 2.17 ± 0.20 | 2.11 ± 0.25 | 2.06 ± 0.12 | 1.98 ± 0.27 | 0.59 | 0.75 | 0.95 |
MS IMIN (mm4) | 1.07 ± 0.12 | 1.29 ± 0.07 | 1.25 ± 0.06 | 1.09 ± 0.13 | 0.91 | 0.77 | 0. 09 |
MS J (mm4) | 3.24 ± 0.31 | 3.40 ± 0.31 | 3.31 ± 0.17 | 3.07 ± 0.40 | 0.67 | 0.90 | 0.53 |
Genistein treatment decreased the total area of the proximal epiphysis of the femur but not the volume of bone
Genistein treatment had no effect on histomorphometric measures of bone strength
Genistein treatment decreased the length of the femur
Genistein treatment increased the amount of force needed to fracture the femur
Lean STD | Lean + GEN | Obese STD | Obese + GEN | Genotype effect (P) | Treatment effect (P) | Genotype * treatment interaction (P) | |
---|---|---|---|---|---|---|---|
Midshaft A-P diameter (mm) | 2.14 ± 0.05 | 2.19 ± 0.07 | 2.09 ± 0.02 | 2.15 ± 0.05 | 0.42 | 0.32 | 0.86 |
Ultimate force (N) | 13.1 ± 1.47 | 16.5 ± 1.27 | 7.4 ± 1.34 | 12.2 ± 1.61 | 0.02 | 0.01 | 0.63 |
Deformation to failure (mm) | 0.36 ± 0.02 | 0.52 ± 0.05 | 0.32 ± 0.02 | 0.46 ± 0.10 | 1.00 | 0.01 | 0.50 |
Time to failure (s) | 3.00 ± 0.55 | 3.60 ± 0.24 | 2.60 ± 0.24 | 3.40 ± 0.68 | 0.53 | 0.16 | 0.83 |
Fracture location (% femur length) | 48.3 ± 12.6 | 49.9 ± 9.4 | 38.4 ± 8.57 | 45.3 ± 10.0 | 0.49 | 0.69 | 0.80 |