Background
Methods
Patients
Characteristics | Number of cases | ALDH1A1 Low | ALDH1A1 High | P-Value |
---|---|---|---|---|
Total
| 97 | 59 | 38 | |
Age
| ||||
< mean 65 years | 46 | 25 | 21 | 0.215 |
≥ mean 65 years | 51 | 34 | 17 | |
Gender
| ||||
Female | 44 | 30 | 14 | 0.18 |
Male | 53 | 29 | 24 | |
Lymph node status
| ||||
N0 | 18 | 11 | 7 | 0.98 |
N1 | 79 | 48 | 31 | |
Grading
| ||||
1 | 4 | 2 | 2 | 0.83 |
2 | 57 | 34 | 23 | |
3 | 36 | 23 | 13 | |
AJCC tumour stage
| ||||
2 | 18 | 11 | 7 | 0.18 |
3 | 74 | 43 | 31 | |
4 | 5 | 5 | 0 | |
R-Status
| ||||
88 | 53 | 35 | 0.70 | |
9 | 6 | 3 |
Immunohistochemistry
Cell lines
Total RNA isolation and real-time qPCR
Western blotting
Statistical analysis
Results
Evaluation of specificity of ALDH1A1 immunohistochemistry
Expression of ALDH1A1 in pancreatic cell lines
Expression of ALDH1A1 and Ki-67 in normal pancreatic tissue and pancreatic cancer cells
Relationship between the expression of ALDH1A1 and Ki-67
Low expression of ALDH1A1 is an independent prognostic marker in pancreatic cancer
Characteristics | RFS* | OAS | ||||
---|---|---|---|---|---|---|
No. of Cases | Time (months) | P-Value | No. Of cases | Time (months) | P-Value | |
Adjuvant Chemotherapy†
| ||||||
Not received | 14 | 7 | 0.6 | 18 | 4.5 |
0.0001
|
Received | 65 | 15 | 69 | 22.8 | ||
Lymph node status
| ||||||
N0 | 16 | 24 | 0.34 | 18 | 32.2 |
0.04
|
N1 | 64 | 12 | 78 | 17.7 | ||
AJCC tumour stage
| ||||||
2 | 16 | 24 | 0.28 | 18 | 32.2 |
0.001
|
3 | 59 | 12 | 73 | 18.7 | ||
4 | 5 | 6 | 5 | 10.9 | ||
ALDH1A1
| ||||||
Low | 47 | 11 |
0.029
| 58 | 16.3 |
0.012
|
High | 33 | 17 | 38 | 32.3 |
Characteristics | Beta | Standard Error | Wald | Df | Relative Risk | 95% CI of Relative Risk | P- Value |
---|---|---|---|---|---|---|---|
ALDH1A1 | |||||||
Expression negative/low vs. High | -0.923 | 0.293 | 9.964 | 1 | 0.397 | 0.224-0.705 |
0.002
|
AJCC stage | 0.891 | 0.288 | 9.592 | 1 | 2.438 | 1.387-4.286 |
0.002
|
Adjuvant chemotherapy vs. no adjuvant chemotherapy | -1.813 | 0.311 | 33.916 | 1 | 0.163 | 0.089-0.300 |
0.0001
|
Subgroup analysis of ALDH1A1 expression in patients with adjuvant chemotherapy and no adjuvant treatment
Characteristics | Beta | Standard-Error | Wald | Relative Risk | 95% CI Relative Risk | P-Value |
---|---|---|---|---|---|---|
ALDH1A1 | ||||||
expression low vs. high | 0.018 | 0.003 | 37.259 | 1.018 | 1.012-1.024 |
0.0001
|
AJCC stage | 0.843 | 0.313 | 7.233 | 2.324 | 1.257-4.295 |
0.007
|