nach oben

International Journal of Legal Medicine

Erschienen in:

01.01.2012 | Technical Note

Method to predict the chance of developing a male profile out of mixtures of male and female DNA

verfasst von: Patrick Johannes Laberke, Rahel Grossenbacher, Roland Hausmann, Beate Balitzki

Erschienen in: International Journal of Legal Medicine | Ausgabe 1/2012

Einloggen, um Zugang zu erhalten

Abstract

In forensic examination it is a standard to take vaginal swabs from victims of sexual assault for further molecular genetic analysis. Laboratories then are usually confronted with mixtures of lots of female and only a small amount of male DNA. Nowadays it is possible to work with specific Y chromosomal markers after DNA extraction by differential lysis. The determined ratio of autosomal DNA and Y chromosomal DNA can be used to identify the possibility of generating a male profile in these samples.

Jänisch S, Meyer H, Germerott T, Albrecht UV, Schulz Y, Debertin AS (2010) Analysis of clinical forensic examination reports on sexual assault. Int J Legal Med 124:227–235PubMedCrossRef

Allery JP, Telmon N, Mieusset R, Blanc A, Rougé D (2001) Cytological detection of spermatozoa: comparison of three staining methods. J Forensic Sci 46(2):349–351PubMed

Benschop CCG, Wiebosch DC, Kloosterman AD, Sijen T (2010) Post-coital vaginal sampling with nylon flocked swabs improves DNA typing. Forensic Sci Int Genet 4(2):115–121PubMedCrossRef

Kimes D, Tahir M (1985) An extraction procedure for seminal/vaginal stains to eliminate streaking in the electrophoresis phosphoglucomutase. Crime Laboratory Digest 12:32–33

Wiegand P, Schürenkamp M, Schütte U (1992) DNA extraction from mixtures of body fluid using mild preferential lysis. Int J Legal Med 104:359–360PubMedCrossRef

Yoshida K, Sekiguchi K, Mizuno N, Kasai K, Sakai H, Sato H, Seta S (1995) The modified method of two-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen. Forensic Sci Int 72(1):25–33PubMedCrossRef

Butler JM (2007) Short tandem repeat typing technologies used in human identity testing. Biotechniques 43(4):ii–vPubMedCrossRef

Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318(6046):577–579PubMedCrossRef

Wiegand P, Heimbold C, Klein R, Immel U, Stiller D, Klintschar M (2010) Transfer of biological stains from different surfaces. Int J Legal Med. doi:10.1007/s004140100424x

10.

Kreike J, Lehner A (1995) Sex determination and DNA competition in the analysis of forensic mixed satins by PCR. Int J Legal Med 107:235–238PubMedCrossRef

11.

Krenke BE, Nassif N, Sprecher CJ, Knox C, Schwandt M, Storts DR (2008) Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA. Forensic Sci Int 3:14–21CrossRef

Titel: Method to predict the chance of developing a male profile out of mixtures of male and female DNA
verfasst von: Patrick Johannes Laberke
Rahel Grossenbacher
Roland Hausmann
Beate Balitzki
Publikationsdatum: 01.01.2012
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 1/2012
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-011-0615-0

Springer Medizin

Method to predict the chance of developing a male profile out of mixtures of male and female DNA

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2012

Development and validation for identity testing of I-DNADuo, a combination of I-DNA1 and a new multiplex system, I-DNA2

Distribution of artifactual gas on post-mortem multidetector computed tomography (MDCT)

Good shedder or bad shedder—the influence of skin diseases on forensic DNA analysis from epithelial abrasions

Comparison of three methods of DNA extraction from human bones with different degrees of degradation

Quantification of forces required for stabbing with screwdrivers and other blunter instruments

Visualisation of the temporary cavity by computed tomography using contrast material

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie