Background
Methods
Microarray dataset
Data preprocessing and DEGs screening
Functional and pathway enrichment analysis
DEGs annotation
PPI network construction
Transcriptional regulatory network construction
Results
Identification of DEGs
DEGs | Gene symbol | FDR | Log2 FC |
---|---|---|---|
Up-regulated |
AXL
| 9.39E−07 | 1.846031 |
SERPINE1
| 8.58E−07 | 1.741651 | |
ITGB1
| 6.28E−08 | 1.739866 | |
PRPF31
| 6.28E−08 | 1.644503 | |
TXNDC5
| 3.26E−08 | 1.629988 | |
WDFY1
| 3.26E−08 | 1.622947 | |
AXL
| 1.57E−07 | 1.554728 | |
SLC1A3
| 5.96E−08 | 1.484443 | |
SET
| 3.90E−07 | 1.477058 | |
ITGB1
| 2.66E−07 | 1.466634 | |
Down−regulated |
AKR1B10
| 3.26E−08 | −2.19537 |
SLC2A3
| 6.28E−08 | −2.01825 | |
HMOX1
| 6.28E−08 | −1.58464 | |
CCND1
| 9.30E−08 | −1.49158 | |
HIST1H2BK
| 1.16E−07 | −1.38961 | |
STX3
| 3.36E−07 | −1.2468 | |
TDG
| 8.98E−08 | −1.23629 | |
SRXN1
| 8.97E−07 | −1.22479 | |
DICER1
| 5.00E−07 | −1.20817 | |
STK40
| 9.14E−07 | −1.19625 |
Functional and pathway enrichment analysis
GO ID | Description | Gene number |
p value | Gene symbols |
---|---|---|---|---|
(A) | ||||
GO:0006366 | Transcription from RNA polymerase II promoter | 47 | 1.01E−03 |
SOX21, TCF25, TOP2A, GTF2F2, CIAO1, SERPINE1, DKK1, CYR61, SOX18, PAF1…
|
GO:0007010 | Cytoskeleton organization | 32 | 2.76E−04 |
PTK2, DPYSL2, CNN3, BICD2, CLIC4, CTGF, EDN1, NRAS, ITGB1, RHOG…
|
GO:0006897 | Endocytosis | 23 | 2.57E−05 |
PTK2, PIK3R2, THBS1, SERPINE1, DKK1, CYFIP2, AXL, RABEPK, LRP1B, ABCA1…
|
GO:0071375 | Cellular response to peptide hormone stimulus | 15 | 5.75E−04 |
PTK2, PIK3R2, GNG10, PPM1A, GNG5, PIK3R1, ATP6V1G1, NRAS, SOCS2, GNG12…
|
GO:0000398 | mRNA splicing, via spliceosome | 10 | 1.02E−02 |
PABPC1, GTF2F2, LSM7, LSM3, POLR2C, UPF3B, MBNL2, C1QBP, PRPF31, PAPOLA
|
GO:0048469 | Cell maturation | 9 | 8.96E−04 |
SOX18, AXL, GJA1, DLD, FOXO3,TYMS,CLN5,EPAS1,PTBP3
|
GO:0043200 | Response to amino acid stimulus | 7 | 6.71E−04 |
CTGF, EDN1, CEBPB, TYMS, CCL2, LAMTOR3, LAMTOR1
|
GO:0006112 | Energy reserve metabolic process | 7 | 4.38E−02 |
GNG10, GNG5, GFPT2, RAP1B, PPP1CC, GNG12, PYGB
|
GO:0018279 | Protein N-linked glycosylation via asparagine | 6 | 1.02E−02 |
UGGT1, MLEC, GFPT2, B4GALT5, PGM3, STT3B
|
GO:0006261 | DNA-dependent DNA replication | 6 | 1.49E−02 |
POLB, MCM3, RFC5, TOP2A, BAZ1A, RPAIN
|
(B) | ||||
GO:0007167 | Enzyme-linked receptor protein signaling pathway | 19 | 2.89E−03 |
KANK1, RTN4, ATP6V1D, PTPRK, EEF2K, ERRFI1, CGN, TGFBR2, ATP6V0A1, MVP…
|
GO:0043588 | Skin development | 9 | 4.97E−03 |
PTHLH, ALDH3A2, ERRFI1, YAP1, STK4, EMP1, COL5A2, NCOA3, DICER1
|
GO:0030330 | DNA damage response, signal transduction by p53 class mediator | 7 | 1.41E−04 |
NDRG1, SPRED1, PSME3, CDKN1A, E2F7, CASP2, HIPK2
|
GO:0001890 | Placenta development | 7 | 4.74E−04 |
TXNRD1, ADM, CCNF, SPP1, STK4, NDP, E2F7
|
GO:0031100 | Organ regeneration | 5 | 6.05E−05 |
ADM, TGFBR2, CCND1, LCP1, CDKN1A
|
GO:0071456 | Cellular response to hypoxia | 5 | 2.26E−03 |
HMOX1, NPEPPS, NDRG1, BNIP3, HIPK2
|
GO:0048002 | Antigen processing and presentation of peptide antigen | 5 | 4.35E−02 |
CTSD, NPEPPS, PSME3, AP1S1, AP1S2
|
GO:0055093 | Response to hyperoxia | 4 | 2.97E−05 |
TXNRD1, BNIP3, CAV1, CDKN1A
|
GO:0000188 | Inactivation of MAPK activity | 4 | 1.36E−04 |
DUSP5, SPRED1, CAV1, DUSP22
|
GO:0060443 | Mammary gland morphogenesis | 4 | 2.15E−03 |
PTHLH, TGFBR2, CAV1, NCOA3
|
KEGG ID | Name | Gene number |
p value | Gene symbols |
---|---|---|---|---|
(A) | ||||
4062 | Chemokine signaling pathway | 12 | 1.63E−03 |
PTK2, PIK3R2, GNG10, GNG5, RAP1B, PIK3R1, NRAS, IL8, GNG12, CSK, FOXO3, CCL2
|
4510 | Focal adhesion | 11 | 7.54E−03 |
PTK2, PIK3R2, THBS1, THBS2, RAP1B, PPP1CC, PIK3R1, ITGB1, ACTG1, FLNB, CAV2
|
4810 | Regulation of actin cytoskeleton | 11 | 1.18E−02 |
PTK2, PIK3R2, CYFIP2, PPP1CC, PIK3R1, NRAS, ITGB1, GNG12, ACTG1, CSK, ARHGEF6
|
4910 | Insulin signaling pathway | 9 | 5.22E−03 |
PIK3R2, PPP1CC, PIK3R1, NRAS, SOCS2, PTPN1, PYGB, CALM2, PTPRF
|
3013 | RNA transport | 9 | 9.27E−03 |
PABPC1, EIF3A, NUP54, EIF3G, UPF3B, NUP155, KPNB1, NUP37, EIF2S3
|
4145 | Phagosome | 8 | 2.82E−02 |
TAP1, THBS1, THBS2, ATP6V1G1, ITGB1, ACTG1, LAMP2, DYNC1LI2
|
5100 | Bacterial invasion of epithelial cells | 7 | 1.24E−03 |
PTK2, PIK3R2, PIK3R1, ITGB1, RHOG, ACTG1, CAV2
|
5142 | Chagas disease (American trypanosomiasis) | 7 | 1.13E−02 |
PIK3R2, SERPINE1, GNA11, PIK3R1, IL8, IFNGR1, CCL2
|
4722 | Neurotrophin signaling pathway | 7 | 3.05E−02 |
PIK3R2, RAP1B, PIK3R1, NRAS, CALM2, CSK, FOXO3
|
4360 | Axon guidance | 7 | 3.28E−02 |
PTK2, DPYSL2, SEMA4F, NRAS, ITGB1, SLIT2, EFNA1
|
5131 | Shigellosis | 6 | 3.01E−03 |
ITGB1, IL8, RHOG, ACTG1, FBXW11, CSK
|
5211 | Renal cell carcinoma | 5 | 2.45E−02 |
PIK3R2, RAP1B, PIK3R1, NRAS, EPAS1
|
5412 | Arrhythmogenic right ventricular cardiomyopathy (ARVC) | 5 | 3.03E−02 |
ITGB1, DAG1, GJA1, ACTG1, CDH2
|
5410 | Hypertrophic cardiomyopathy | 5 | 4.62E−02 |
TPM3, ITGB1, DAG1, TPM1, ACTG1
|
4350 | TGF-beta signaling pathway | 5 | 4.83E−02 |
THBS1, THBS2, SMAD6, ID3, SMAD5
|
20 | Citrate cycle (TCA cycle) | 4 | 5.11E−03 |
CS, DLD, DLAT, SDHA
|
5144 | Malaria | 4 | 3.19E−02 |
THBS1, THBS2, IL8, CCL2
|
5213 | Endometrial cancer | 4 | 3.39E−02 |
PIK3R2, PIK3R1, NRAS, FOXO3
|
5223 | Non-small cell lung cancer | 4 | 3.82E−02 |
PIK3R2, PIK3R1, NRAS, FOXO3
|
3410 | Base excision repair | 3 | 4.23E−02 |
POLB, PARP1, PARP3
|
(B) | ||||
4144 | Endocytosis | 9 | 4.62E−04 |
ASAP2, VPS36, TGFBR2, ASAP1, CAV1, SH3KBP1, EHD1, RAB22A, DNM3
|
4142 | Lysosome | 7 | 4.55E−04 |
CTSD, TPP1, ATP6V0A1, ABCB9, AP1S1, AP1S2, NEU1
|
2010 | ABC transporters | 4 | 1.58E−03 |
ABCC2, ABCC3, ABCB9, ABCC5
|
10 | Glycolysis/gluconeogenesis | 4 | 6.56E−03 |
ENO2, ALDH3A2, PGAM1, PGK1
|
5220 | Chronic myeloid leukemia | 4 | 9.85E−03 |
TGFBR2, CCND1, CDKN1A, BCL2L1
|
561 | Glycerolipid metabolism | 3 | 1.98E−02 |
ALDH3A2, AGPAT9, LCLAT1
|
5212 | Pancreatic cancer | 3 | 4.69E−02 |
TGFBR2, CCND1, BCL2L1
|
4966 | Collecting duct acid secretion | 2 | 3.85E−02 |
ATP6V1D, ATP6V0A1
|
650 | Butanoate metabolism | 2 | 4.67E−02 |
AKR1B10, HMGCS1
|
The annotation of DEGs
DEGs | TF numbers | TFs | TAG numbers | TAGs | ||
---|---|---|---|---|---|---|
TSGs | Oncogenes | Others | ||||
Up-regulated | 1 |
KLF12
| 33 |
BAP1, THBS1, DKK1, PAF1, ST13, LRP1B, PDGFRL, ITGB1, TPM1, GJA1, CDH11, SLIT2, GLIPR1, FAT1, SOD2, FOXO3, EFNA1, GAS1, PTPRF, RAD51C, CAV2, SDHA
|
SET, CCNA2, AXL, NRAS, ROS1, SCK
|
GTF2F2, CTGF, FHL2, C1QBP, CCL2
|
Down-regulated | 8 |
ASCL1, ETV4, HSF1, LMO3, PML, RUNX3, TCF7, USF2
| 21 |
HIPK2, YAP1, ERRFI1, PTPRK, KANK1, BNIP3L, DUSP22, SASH1, CDKN1A, NDRG4, ZFHX3, NDRG1, TGFBR2, |
BCL2L2, NCOA3, CCND1, CDC25B
|
PTHLH, EMP1, CAV1, GLS
|
PPI network analysis
Gene | Degree | Gene | Degree | Gene | Degree | Gene | Degree |
---|---|---|---|---|---|---|---|
CCND1
| 29 |
SOD2
| 19 |
CENPN
| 16 |
KIF11
| 15 |
PIK3R1
| 25 |
TYMS
| 19 |
CAV1
| 16 |
PTK2
| 15 |
PGK1
| 22 |
CDKN1A
| 18 |
PIK3R2
| 16 |
EDN1
| 14 |
NUP37
| 22 |
PARP1
| 18 |
CTGF
| 15 |
CS
| 13 |
CALM2
| 21 |
TOP2A
| 18 |
RFC5
| 15 |
CCL2
| 13 |
MCM3
| 21 |
ITGB1
| 18 |
NUP155
| 15 |
RSL24D1
| 12 |
GMNN
| 20 |
TCP1
| 18 |
NRAS
| 15 |
CDCA7
| 12 |
CCNA2
| 20 |
SERPINE1
| 17 |
NIP7
| 15 |
BCL2L1
| 12 |