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Acta Neurologica Belgica

Erschienen in:

05.04.2023 | Original article

miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury

verfasst von: Meng Wang, Xin Yu, Bin Li, Chensong Gao, Yan Chen, Xiaoyang Zhang, Wenling Li, Lijun Yang, Zhenzeng Fan

Erschienen in: Acta Neurologica Belgica | Ausgabe 4/2023

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Abstract

Objective

The abnormal expression of matrix metalloproteinase 9 (MMP9) and Aquaporin 4 (AQP4) closely associates with the traumatic brain injury (TBI) development.

Methods

Here, we investigated the relationship between miR-211-5p and MMP9/AQP4 axis in TBI patients and astrocyte cells. Demographics, clinical features, and cerebrospinal fluid (CSF) samples were collected from traumatic brain injury (TBI) patients (n = 96) and controls (n = 30) for pathological and gene expression analyses. Luciferase activity assay and gene expression analyses were performed to dissect the regulatory mechanism of miR-211-5p on MMP9/AQP4 in human astrocyte cells.

Results

miR-211-5p mRNA was significantly decreased in the CSF of TBI patients, which positively correlated with the expression of both MMP9 and AQP4. miR-211-5p could target MMP9 directly in SVG P12 cells. Overexpression of miR-211-5p decreased the expression of MMP9, on the contrary, knockdown miR-211-5p through inhibitors increased the expression of both MMP9 and AQP4.

Conclusion

miR-211-5p inhibits the MMP9/AQP4 axis in human astrocyte cells, which represents a promising approach for the TBI treatment.

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Titel: miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury
verfasst von: Meng Wang
Xin Yu
Bin Li
Chensong Gao
Yan Chen
Xiaoyang Zhang
Wenling Li
Lijun Yang
Zhenzeng Fan
Publikationsdatum: 05.04.2023
Verlag: Springer International Publishing
Erschienen in: Acta Neurologica Belgica / Ausgabe 4/2023
Print ISSN: 0300-9009
Elektronische ISSN: 2240-2993
DOI: https://doi.org/10.1007/s13760-023-02205-1

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