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Archives of Virology

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01.07.2013 | Annotated Sequence Record

Molecular characterisation of two divergent variants of grapevine leafroll-associated virus 3 in New Zealand

verfasst von: Kar Mun Chooi, Daniel Cohen, Michael N. Pearson

Erschienen in: Archives of Virology | Ausgabe 7/2013

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Abstract

Partial genomic sequences of two divergent grapevine leafroll-associated virus 3 (GLRaV-3) variants, NZ1-B and NZ2, from New Zealand were determined and analysed (11,827 nt and 7,612 nt, respectively). At the nucleotide level, both variants are more than 20 % different from the previously published GLRaV-3 sequences, from phylogenetic groups 1 to 5. Phylogenetic analysis indicated that NZ1-B is a variant of the previously identified divergent NZ-1, while NZ2 is a novel sequence with only 76 % nucleotide sequence identity to GLRaV-3 variants NZ-1, GH11, and GH30. Therefore, NZ2 is a new variant of GLRaV-3. Amino acid sequence analysis of the NZ1-B and NZ2 coat proteins indicated significant substitutions that are predicted to alter the coat protein structure, which potentially leads to the observed reduced immunological reactivity of both variants to the Bioreba anti-GLRaV-3 conjugated monoclonal antibody.

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Titel: Molecular characterisation of two divergent variants of grapevine leafroll-associated virus 3 in New Zealand
verfasst von: Kar Mun Chooi
Daniel Cohen
Michael N. Pearson
Publikationsdatum: 01.07.2013
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1631-9

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