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Erschienen in: Current Cardiology Reports 11/2017

01.11.2017 | Heart Failure (HJ Eisen, Section Editor)

Molecular Diagnostic Testing in Cardiac Transplantation

verfasst von: Kiran Khush, Shirin Zarafshar

Erschienen in: Current Cardiology Reports | Ausgabe 11/2017

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Abstract

Purpose of Review

Acute rejection is one of the most feared complications of cardiac transplantation. Developing non-invasive methods for detection and surveillance of acute rejection have long been a goal for post-transplant care.

Recent Findings

Here, we will review molecular diagnostic tests that are currently in use or under development to diagnose acute cellular rejection after cardiac transplantation.

Summary

Gene expression, microRNA, molecular microscope, and cell-free DNA assays offer non-invasive alternatives to the endomyocardial biopsy for acute rejection surveillance.
Literatur
2.
3.
Zurück zum Zitat Melvin KR, Mason JW. Endomyocardial biopsy: its history, techniques and current indications. Can Med Assoc J. 1982;126(12):1381–6.PubMedPubMedCentral Melvin KR, Mason JW. Endomyocardial biopsy: its history, techniques and current indications. Can Med Assoc J. 1982;126(12):1381–6.PubMedPubMedCentral
4.
Zurück zum Zitat Crespo-Leiro MG, et al. Concordance among pathologists in the second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II). Transplantation. 2012;94(11):1172–7.CrossRefPubMed Crespo-Leiro MG, et al. Concordance among pathologists in the second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II). Transplantation. 2012;94(11):1172–7.CrossRefPubMed
5.
Zurück zum Zitat Patel PC, et al. High-sensitivity cardiac troponin I assay to screen for acute rejection in patients with heart transplant. Circulation: HF. 2014;7:463–9. Patel PC, et al. High-sensitivity cardiac troponin I assay to screen for acute rejection in patients with heart transplant. Circulation: HF. 2014;7:463–9.
6.
Zurück zum Zitat Horwitz PA, Tsai EJ, Putt ME, et al. Detection of cardiac allograft rejection and response to immunosuppressive therapy with peripheral blood gene expression. Circulation. 2004;110:3815–21.CrossRefPubMed Horwitz PA, Tsai EJ, Putt ME, et al. Detection of cardiac allograft rejection and response to immunosuppressive therapy with peripheral blood gene expression. Circulation. 2004;110:3815–21.CrossRefPubMed
7.
Zurück zum Zitat Schoels M, Dengler TJ, Richter R, Meuer SC, Giese T. Detection of cardiac allograft rejection by real-time PCR analysis of circulating mononuclear cells. Clin Transpl. 2004;18:513–7.CrossRef Schoels M, Dengler TJ, Richter R, Meuer SC, Giese T. Detection of cardiac allograft rejection by real-time PCR analysis of circulating mononuclear cells. Clin Transpl. 2004;18:513–7.CrossRef
8.
Zurück zum Zitat Deng, et al. Noninvasive discrimination of rejection in cardiac allograft recipients using gene expression profiling. Am J Transplant. 2006;6(1):150–60.CrossRefPubMed Deng, et al. Noninvasive discrimination of rejection in cardiac allograft recipients using gene expression profiling. Am J Transplant. 2006;6(1):150–60.CrossRefPubMed
9.
Zurück zum Zitat Starling RC, et al. Molecular testing in the management of cardiac transplant recipients: initial clinical experience. J Heart Lung Transplant. 2006;25(12):1389–95.CrossRefPubMed Starling RC, et al. Molecular testing in the management of cardiac transplant recipients: initial clinical experience. J Heart Lung Transplant. 2006;25(12):1389–95.CrossRefPubMed
10.
Zurück zum Zitat Pham MX, et al. Gene-expression profiling for rejection surveillance after cardiac transplantation. NEJM. 2010;362:1890–900.CrossRefPubMed Pham MX, et al. Gene-expression profiling for rejection surveillance after cardiac transplantation. NEJM. 2010;362:1890–900.CrossRefPubMed
11.
Zurück zum Zitat • Crespo-Leiro MG, et al. Clinical usefulness of gene-expression profile to rule out acute rejection after heart transplantation: CARGO II. Eur Heart J. 2016;37(33):2591–601. The CARGO II study compared gene expression profiling to standard endomyocardial biopsy for detection of acute cellular rejection CrossRefPubMedPubMedCentral • Crespo-Leiro MG, et al. Clinical usefulness of gene-expression profile to rule out acute rejection after heart transplantation: CARGO II. Eur Heart J. 2016;37(33):2591–601. The CARGO II study compared gene expression profiling to standard endomyocardial biopsy for detection of acute cellular rejection CrossRefPubMedPubMedCentral
12.
Zurück zum Zitat Kobashigawa JA, et al. Randomized pilot trial of gene expression profiling versus heart biopsy in the first year after heart transplant: Early Invasive monitoring attenuation through gene expression trial (EIMAGE). Circ Heart Fail. 2015;8:557–64.CrossRefPubMed Kobashigawa JA, et al. Randomized pilot trial of gene expression profiling versus heart biopsy in the first year after heart transplant: Early Invasive monitoring attenuation through gene expression trial (EIMAGE). Circ Heart Fail. 2015;8:557–64.CrossRefPubMed
13.
Zurück zum Zitat Li L, et al. Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. PLoS One. 2013;8(12):e82153.CrossRefPubMedPubMedCentral Li L, et al. Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. PLoS One. 2013;8(12):e82153.CrossRefPubMedPubMedCentral
14.
Zurück zum Zitat • Loupy A, et al. Gene expression profiling for the identification and classification of antibody-mediated heart rejection. Circulation. 2017;135:917–35. Loupy and colleagues demonstrate how gene expression profiling can also be used for surveillance of antibody-mediated rejection CrossRefPubMed • Loupy A, et al. Gene expression profiling for the identification and classification of antibody-mediated heart rejection. Circulation. 2017;135:917–35. Loupy and colleagues demonstrate how gene expression profiling can also be used for surveillance of antibody-mediated rejection CrossRefPubMed
15.
Zurück zum Zitat Bodez D, et al. Myocardial gene expression profiling to predict and identify cardiac allograft acute cellular rejection: the GET-study. PLoS One. 2016;11(110):e0167213.CrossRefPubMedPubMedCentral Bodez D, et al. Myocardial gene expression profiling to predict and identify cardiac allograft acute cellular rejection: the GET-study. PLoS One. 2016;11(110):e0167213.CrossRefPubMedPubMedCentral
16.
Zurück zum Zitat Hollander Z, et al. Predicting acute cardiac rejection from donor heart and pre-transplant recipient blood gene expression. J Heart Lung Transplant. 2013;32(2):259–65.CrossRefPubMed Hollander Z, et al. Predicting acute cardiac rejection from donor heart and pre-transplant recipient blood gene expression. J Heart Lung Transplant. 2013;32(2):259–65.CrossRefPubMed
17.
Zurück zum Zitat Huyen V, et al. MicroRNAs as non-invasive biomarkers of heart transplant rejection. Eur Heart J. 2014;35(45):3194–202.CrossRef Huyen V, et al. MicroRNAs as non-invasive biomarkers of heart transplant rejection. Eur Heart J. 2014;35(45):3194–202.CrossRef
18.
Zurück zum Zitat Dewi IS, et al. Association of serum MiR-142-3p and MiR-101-3p levels with acute cellular rejection after heart transplantation. PLoS One. 2017;12(1):e0170842.CrossRef Dewi IS, et al. Association of serum MiR-142-3p and MiR-101-3p levels with acute cellular rejection after heart transplantation. PLoS One. 2017;12(1):e0170842.CrossRef
19.
Zurück zum Zitat Loupy A, et al. Molecular microscope strategy to improve risk stratification in early antibody-mediated kidney allograft rejection. J Am Soc Nephrol. 2014;25(10):2267–77.CrossRefPubMedPubMedCentral Loupy A, et al. Molecular microscope strategy to improve risk stratification in early antibody-mediated kidney allograft rejection. J Am Soc Nephrol. 2014;25(10):2267–77.CrossRefPubMedPubMedCentral
22.
Zurück zum Zitat • De Vlaminck I, et al. Circulating cell-free DNA enables noninvasive diagnosis of heart transplant rejection. Sci Transl Med. 2014;6(241):241ra77. De Vlaminck and colleagues show that cell-free DNA can detect acute cellular and antibody-mediated rejection CrossRefPubMedPubMedCentral • De Vlaminck I, et al. Circulating cell-free DNA enables noninvasive diagnosis of heart transplant rejection. Sci Transl Med. 2014;6(241):241ra77. De Vlaminck and colleagues show that cell-free DNA can detect acute cellular and antibody-mediated rejection CrossRefPubMedPubMedCentral
23.
Zurück zum Zitat Grskovic M, et al. Validation of a clinical-grade assay to measure donor-derived cell-free DNA in solid organ transplant recipients. J Mol Diagn. 2016;18(6):890–902.CrossRefPubMed Grskovic M, et al. Validation of a clinical-grade assay to measure donor-derived cell-free DNA in solid organ transplant recipients. J Mol Diagn. 2016;18(6):890–902.CrossRefPubMed
24.
Zurück zum Zitat Crespo-Leiro MG, et al. Increased plasma levels of donor-derived cell-free DNA correlate with rejection in heart transplant recipients: the CARGO II multicenter trial. JHLT. 2015;34(4):S31032. Crespo-Leiro MG, et al. Increased plasma levels of donor-derived cell-free DNA correlate with rejection in heart transplant recipients: the CARGO II multicenter trial. JHLT. 2015;34(4):S31032.
25.
Zurück zum Zitat Kobashigawa JA, et al. Initial analysis of the donor-derived cell-free DNA-outcomes AlloMap registry (D-OAR) study in heart transplant recipients undergoing surveillance for rejection. JHLT. 2016;35(4):S33. Kobashigawa JA, et al. Initial analysis of the donor-derived cell-free DNA-outcomes AlloMap registry (D-OAR) study in heart transplant recipients undergoing surveillance for rejection. JHLT. 2016;35(4):S33.
Metadaten
Titel
Molecular Diagnostic Testing in Cardiac Transplantation
verfasst von
Kiran Khush
Shirin Zarafshar
Publikationsdatum
01.11.2017
Verlag
Springer US
Erschienen in
Current Cardiology Reports / Ausgabe 11/2017
Print ISSN: 1523-3782
Elektronische ISSN: 1534-3170
DOI
https://doi.org/10.1007/s11886-017-0915-1

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