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European Journal of Trauma and Emergency Surgery

Erschienen in:

12.03.2019 | Original Article

Monotrauma is associated with enhanced remote inflammatory response and organ damage, while polytrauma intensifies both in porcine trauma model

verfasst von: Philipp Störmann, Nils Wagner, Kernt Köhler, Birgit Auner, Tim-P. Simon, Roman Pfeifer, Klemens Horst, Hans-Christoph Pape, Frank Hildebrand, Sebastian Wutzler, Ingo Marzi, Borna Relja

Erschienen in: European Journal of Trauma and Emergency Surgery | Ausgabe 1/2020

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Abstract

Aim

Severely injured patients experience substantial immunological stress upon traumatic insult. Next to the direct local tissue injury also other organs, which are not directly injured such as liver and lung, are frequently affected by a so-called remote organ damage (ROD) after trauma. Thus, we studied the inflammatory response of lung and liver either after isolated femur fracture as example for ROD, or after multiple trauma in a porcine polytrauma model.

Methods

Twenty-four male pigs (Sus scrofa) underwent either isolated standardized femoral fracture (monotrauma, MT, n = 12) or polytrauma (PT, n = 12). PT consisted of a femur fracture, lung contusion, liver laceration, hemorrhagic shock, subsequent resuscitation and surgical fracture fixation. Six animals served as controls (sham). After 72 h inflammatory changes were determined by analyses of the interleukin (IL)-6 gene expression and tissue infiltration of polymorphonuclear leukocyte (PMN, myeloperoxidase staining). ROD in MT, and lung as well as liver damage in PT were assessed histologically by hematoxylin–eosin staining. Expression of phosphorylated p65 NF-κB was evaluated by immunohistology.

Results

IL-6 increased in lungs and liver in both groups MT and PT, respectively, compared to sham. Similarly, PMN infiltration of the lungs and liver increased significantly after both MT and PT compared to sham. Histological evaluation demonstrated tissue damage notably in lungs after MT, while tissue damage after PT was found in both lung and liver after PT. p65 NF-κB tended to an increase upon MT, and was significantly enhanced after PT in both tissues.

Conclusion

Our data indicate that remote organ damage after MT notably in lungs was associated with an enhanced inflammatory response. Severe polytrauma substantially intensifies this response and organ damage in the underlying model.

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Titel: Monotrauma is associated with enhanced remote inflammatory response and organ damage, while polytrauma intensifies both in porcine trauma model
verfasst von: Philipp Störmann
Nils Wagner
Kernt Köhler
Birgit Auner
Tim-P. Simon
Roman Pfeifer
Klemens Horst
Hans-Christoph Pape
Frank Hildebrand
Sebastian Wutzler
Ingo Marzi
Borna Relja
Publikationsdatum: 12.03.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Trauma and Emergency Surgery / Ausgabe 1/2020
Print ISSN: 1863-9933
Elektronische ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-019-01098-1

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Monotrauma is associated with enhanced remote inflammatory response and organ damage, while polytrauma intensifies both in porcine trauma model

Abstract