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Journal of Clinical Immunology

Erschienen in:

01.07.2009

Oral Tolerization with Cardiac Myosin Peptide (614–629) Ameliorates Experimental Autoimmune Myocarditis: Role of Stat 6 Genes in BALB/CJ Mice

verfasst von: Patricia A. Gonnella, Pedro J. Del Nido, Francis X. McGowan

Erschienen in: Journal of Clinical Immunology | Ausgabe 4/2009

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Abstract

Introduction

Experimental autoimmune myocarditis (EAM) is mediated by myocardial infiltration by myosin-specific T cells secreting inflammatory cytokines.

Materials and methods

To clarify the role of cytokines in EAM, we compared STAT 6-deficient (^−/−) with STAT 4^−/− and wild-type (BALB/CJ) mice following immunization with cardiac myosin peptide (614–629).

Results

Wild-type mice developed severe disease with a small increase in severity in STAT 6^−/− mice, while STAT 4^−/− mice were resistant to EAM. STAT 6^−/− mice had increased splenocyte proliferation and INF-γ production versus wild type, while STAT 4^−/− mice had decreased proliferation and INF-γ. Following oral administration of myosin (614–629), tolerization was induced in wild-type mice evidenced by amelioration of myocarditis and up-regulation of IL-4. Adoptive transfer of splenocytes from orally tolerized mice resulted in inhibition of disease in STAT 6^−/− mice.

Conclusion

These results demonstrate that oral tolerization ameliorates EAM in BALB/CJ mice and indicate a down-regulatory role for STAT 6 genes.

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Titel: Oral Tolerization with Cardiac Myosin Peptide (614–629) Ameliorates Experimental Autoimmune Myocarditis: Role of Stat 6 Genes in BALB/CJ Mice
verfasst von: Patricia A. Gonnella
Pedro J. Del Nido
Francis X. McGowan
Publikationsdatum: 01.07.2009
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 4/2009
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-009-9290-z

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Materials and methods

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