Background
The mammalian aurora kinases aurora-A, aurora-B and aurora-C comprise a family of serine/threonine kinases that are essential for cell cycle control and mitotic progression [
1]. Interest in the auroras has intensified since the observation that both aurora-A and B are over-expressed in a wide variety of tumour types [
2‐
5] including those of leukaemic origin [
6,
7]. The implication of the auroras in tumourigenesis and the fact that that they are kinases, amenable to small molecule inhibition, makes them attractive targets for anticancer drug development. Success of agents such as imatinib in the treatment of chronic myelogenous leukaemia has increased confidence that small-molecule inhibitors of specific kinases may prove to be highly effective anticancer agents [
8]. Despite having high sequence homologies in their kinase domains the three aurora members have very distinct subcellular localizations and functions during mitosis [
9]. Aurora-B is a chromosomal passenger protein which undergoes dynamic localization during mitosis, associating first to the inner centromeric region during prometaphase, and then to the spindle midzone and midbody during late anaphase and telophase suggesting a role in cytokinesis [
1,
10]. Aurora-B is the catalytic component of the chromosomal passenger complex (CPC), which is composed of three additional non-catalytic subunits that direct its activity: survivin, inner centromere protein (INCENP) and borealin. The CPC orchestrates the spindle checkpoint and ensures the accurate segregation of chromatids and correct microtubule/kinetochore attachment during mitosis and cytokinesis [
11]. Aurora-B is also known to phosphorylate Histone H3 (pHH3) at the serine 10 position during mitosis [
12,
13]. Inhibition of Histone H3 phosphorylation has been reported to prevent initiation of chromosome condensation and entry into mitosis [
14]. Aurora-A is known to phosphorylate numerous centrosomal proteins and primarily functions in centrosomal regulation and mitotic spindle formation with loss of Aurora-A function leading to cell cycle arrest and monopolar mitotic spindles [
9]. Aurora-C is the least studied of the aurora family and is highly expressed in the testis where it is thought to have a specific role in the regulation of chromosome segregation during male meiosis [
15]. More recently aurora-c has been identified at low levels in sixteen other tissues including bone marrow with studies suggesting that it has a complementary role to aurora B and Survivin as a chromosomal passenger protein [
16,
17].
A growing number of aurora kinase inhibitors have been described that show anti-tumour activity
in vivo. Three non-selective aurora kinase inhibitors ZM447439, Hesperadin and VX-680 all induce similar phenotypes when tested in cell based assays [
18‐
20]. Specifically, all three inhibit phosphorylation of Histone H3 on serine 10 and induce DNA endoreduplication in the absence of cytokinesis, results that suggest that their cellular effects are largely due to the inhibition of aurora-B [
21]. We have previously reported the same cellular phenotype in AML cell lines treated with barasertib-hQPA [
22].
Barasertib (formerly AZD1152) is a quinazoline prodrug which is converted in plasma to the more active moiety barasertib-hQPA (AZD1152-hQPA) and it is the more active barasertib-hQPA that has been supplied by AstraZeneca for the purpose of this study. Barasertib-hQPA is an aurora kinase inhibitor that has potent selectivity for inhibition of aurora-B (Ki: aurora-B = 0.36 nM) compared to aurora-A and C (K
i: aurora-A = 1369 nM and aurora-C = 17.0 nM) and a panel of 50 other kinases [
23]. We have however recently reported that the FMS-like tyrosine kinase 3 internal tandem duplication (FLT3-ITD) mutation is a secondary target for barasertib-hQPA in AML cells [
22]. Barasertib has been shown to significantly inhibit the growth of human colon, lung and haematological tumour xenografts in immunodeficient mice and as such has been selected for clinical evaluation [
23,
24]. It has also shown tumouricidal activity against a panel of tumour cell lines including those of acute myeloid leukaemia (AML) origin [
22,
25‐
27]. Results of a Phase 1 study in AML were reported at ASH 2010 (Abstract 656) and a Phase II study is ongoing.
AML is a heterogeneous clonal disorder of haemopoietic progenitor cells where both failure to differentiate and over proliferation results in accumulation of non-functional cells termed myeloblasts [
28]. Intrinsic resistance or treatment-induced acquired resistance is one of the major obstacles to the effective treatment of patients with AML. While nearly 80% of younger AML patients may initially achieve complete remission with current therapy most will relapse with resistant disease [
29]. Clinical outcomes in the elderly have been even more modest as these patients do not tend to tolerate intensive chemotherapy regimens and frequently have poor cytogenetics [
30]. Less than 10% of older patients with AML will achieve long-term disease free survival with conventional chemotherapy [
31]. This inability to successfully treat AML patients, particularly the elderly, underlies the continuing need to develop new treatments for AML.
The development of multidrug resistance (MDR) is frequently observed in the treatment of cancer, and is a phenomenon that allows tumour cells that have been exposed to one cytotoxic agent to develop cross resistance to a range of structurally and functionally unrelated compounds. In patients with AML, MDR can be present intrinsically at diagnosis or can arise during chemotherapy as well as at relapse. ATP binding cassette (ABC) transporter-mediated active efflux of cytotoxic agents is the most well characterized mechanism by which cancer cells develop MDR, particularly after repeated cycles of chemotherapy [
32]. The ABC transporters are an evolutionary extremely well conserved family of transmembrane proteins expressed in most cells and involved in the ATP driven transport of a huge variety of substrates including sugars, peptides, inorganic ions, amino acids, proteins, vitamins and metallic ions [
33]. The family currently consists of 49 members, 13 of which are associated with chemotherapeutical drug transport and drug resistance [
34]. The genes can be divided into subfamilies based on similarity in gene structure resulting in seven mammalian ABC gene subfamilies (ABCA, B C, D, E F and G family). The most widely studied members ABCB1 (MDR1/P-glycoprotein), ABCC1 (multidrug resistance protein, MRP1) and ABCG2 (breast cancer resistance protein, BCRP) have the ability to export a wide variety of structurally unrelated chemotherapeutic compounds from cancer cells, thereby conferring MDR to these cell [
33].
Despite improvements accomplished in the last thirty years with the use of combination of cytarabine and intercalating agents, the overall prognosis of adult AML remains poor. One of the best characterized resistance mechanisms in AML is drug extrusion mediated by P-glycoprotein (Pgp). Expression of the MDR1 gene coding for Pgp is high in elderly patients with AML and is associated with worse complete remission rates [
30]. Multidrug resistance-associated protein 1 (MRP1) has also been shown to contribute to MDR in AML [
30], whereas other studies have been unable to detect any correlation between MRP1 protein expression and clinical response in AML patients [
35]. Breast cancer resistance protein (BCRP) has been shown to be overexpressed in 33% of AML patients with normal karyotype and to significantly affect the duration of complete remissions [
36]. Another study showed BCRP expression to be a prognostic factor in AML patients treated with daunorubicin and mitoxantrone but not with idarubicin [
37]. Conversely, another group found a prognostic value for Pgp and MRP1 but suggested BCRP has a limited function in the drug efflux related resistance in AML [
38]. It has recently been reported that the amplification of genes that encode for Pgp and BCRP can confer resistance to barasertib-hQPA in colon and pancreatic carcinoma cell lines [
39].
In this study we will investigate the specificity of barasertib-hQPA in AML cell lines and primary samples in relation to their ABC transporter status.
Discussion
The success of agents such as imatinib in the treatment of chronic myelogenous leukaemia has lead to an increase in confidence that small-molecule inhibitors of specific kinases may prove to be highly effective anticancer agents [
8]. The aurora kinase family are essential for normal mitotic progression and have attracted great interest as potential new therapeutic targets. Overexpression of aurora kinases have been reported in many tumour types and linked to a poor prognosis. The aurora-B kinase specific inhibitor barasertib-hQPA has shown tumouricidal activity against a panel of tumour cell lines and xenograft models including those of AML origin [
22‐
24,
27]. Our group have recently reported that the FLT3-ITD mutation is a secondary target for barasertib-hQPA and that primary AML samples with an ITD mutation in the FLT3 gene are particularly sensitive to the drug [
22]. The FLT3 gene is one of the most commonly mutated genes in AML with the FLT3-ITD mutation appearing in roughly 24% of cases and being associated with a poor prognosis [
46,
47]. Barasertib-hQPA is therefore of potential use as a future treatment in AML.
Intrinsic MDR or treatment-induced MDR has historically been one of the major obstacles to the effective treatment of patients with AML. Elevated expression of the MDR1 gene coding for the drug efflux pump Pgp is one of the best characterized resistance mechanisms in AML with high expression in elderly patients particularly associated with worse complete remission rates [
30]. The ABCG2 gene encoding BCRP has also been shown to be overexpressed in AML patients and to significantly affect the duration of complete remission [
36]. Any potential new drug for the treatment of AML should therefore be screened for its efficacy in patients expressing high levels of these drug transporters.
Aurora-B is known to phosphorylate Histone H3 (pHH3) at the serine 10 position during mitosis [
12,
13]. We have previously demonstrated a method for measuring pHH3 expression in our cell lines and more importantly in our primary samples and its subsequent down-regulation after barasertib-hQPA treatment [
22]. We can therefore use pHH3 expression as a biomarker for barasertib-hQPA activity. Initial experiments showed that our Pgp positive (KG-1a) and BCRP positive (OCI-AML6.2) cell lines were much less sensitive to barasertib-hQPA in comparison to transporter negative cell lines. This was seen at both the biomarker and subsequent viability level. Because we had no parental Pgp negative comparison for the KG-1a cell line we wanted to create a Pgp expressing cell line from the OCI-AML3 cells. The subsequently developed Pgp expressing OCI-AML3DNR cell line was more resistant to barasertib-hQPA compared to the sensitive OCI-AML3 parent cells (Figure
2B). This resistance to barasertib-hQPA in the transporter positive cell lines was reversed when known inhibitors of Pgp and BCRP were co-cultured with barasertib-hQPA (Figure
4). There was an initial spike in KG-1a pHH3 levels at 10 nM barasertib-hQPA with the addition of CSA (Figure
4A(i)). This spike was also seen in the sensitive U937 cells also at 10 nM barasertib-hQPA (Figure
2A(i)) and to a lesser extent in the OCI-AML3 cells (Figure
2B(i)) indicating that CSA is sensitizing the Pgp expressing KG-1a cells to barasertib-hQPA. By using
14[C]-barasertib-hQPA in combination with known Pgp and BCRP inhibitors we show definitively that the drug is being effluxed by these transporters in the OCI-AML3DNR and OCI-AML6.2 cell lines.
Inhibiting Pgp as a way of reversing MDR has been intensively studied for many years. Pharmacological inhibition of Pgp by small-molecule antagonists has been studied in several AML trials with various agents but only yielding little clinical success [
48‐
51]. Many of these agents are substrates for other transporters and enzyme systems resulting in unpredictable pharmacokinetic interactions in the presence of chemotherapy agents. Recently more specific so called third generation inhibitors of MDR have been developed that have the potential to minimize any drug-drug interactions [
52]. A combination of these inhibitors with barasertib-hQPA could circumvent any problem of drug efflux by ABC transporters seen with this drug.
We measured pHH3 expression in 37 primary AML samples cultured for one hour with or without barasertib-hQPA. 9/37 (24.3%) were positive for Pgp and 9/35 (25.7%) were positive for BCRP with a significant correlation (p = 0.008, r2 = 0.238) seen for co-expression. This data agrees with results seen previously [
36]. Pgp positive samples were significantly less sensitive to barasertib-hQPA induced pHH3 inhibition compared to Pgp negative samples (p = 0.001) as were BCRP positive samples compared to BCRP negative samples (p = 0.001). Importantly though, >50% inhibition of pHH3 was still seen in all but 2 primary samples (94.6%). So certainly at the level of the biomarker we see a much better response in ABC transporter positive primary samples than in transporter positive cell lines at the corresponding dose of barasertib-hQPA. Whereas in the transporter positive cell lines we demonstrated efflux of barasertib-hQPA using radio-labelled drug it is clear that all of our primary samples retained enough drug to target the pHH3 biomarker. This difference in response may be explained by the 'artificially' high transporter expression seen in the cell lines. For example, if we look at our measure of Pgp function, the mean CSA modulation ratio in our primary samples was 1.74. Four times this was seen in the Pgp expressing OCI-AML3DNR cell line and greater than seven times seen in the Pgp expressing KG-1a cell line. Likewise the amount of Pgp protein measured using the MRK-16 antibody was two-folds higher in the OCI-AML3DNR cells and seven-folds higher in the KG-1a cells compared to the mean value of 1.13 seen in primary samples.
Methods
Materials
Materials were from Sigma (Poole, Dorset UK) unless otherwise stated. Barasertib-hQPA was provided by AstraZeneca (Cheshire, UK).
Cell lines
The OCI-AML3 myeloid leukaemia cell line was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). U937, HL-60 and KG-1a cell lines were from the European Collection of Animal Cell Cultures (Salisbury, UK). The ABCG2 stably transfected OCI-AML6.2 cells were a gift from Dr. Jo Mountford (University of Glasgow, UK). The HL-60ADR [
43] cell line was a gift from Mark Center (Kansas State University, USA). U937, OCI-AML3 and OCI-AML6.2 cell lines were maintained in RPMI 1640 medium with 10% foetal calf serum (FCS; First Link, Birmingham, UK), 2 mM L-glutamine, 100 U/ml penicillin and 10 μg/ml streptomycin. The KG-1a cell line was maintained as above with 20% FCS. All cultures were kept at 37°C in 5% CO
2 and all experiments were performed with cell lines in log phase. Continued testing to authenticate these cell lines was initially performed using a panel of monoclonal antibodies and later by STR fingerprinting. Mycoplasma testing was carried out routinely using a Mycoalert mycoplasma detection kit (Lonza, Rockland, USA) and following the manufacturer's instructions.
Selection of Daunorubicin resistant OCI-AML3 cells
OCI-AML3 cells were initially exposed to daunorubicin (DNR) at a concentration of 10 nM, which represented the IC50 concentration. Cells were seeded at 5 × 105/ml in 50 ml of complete medium containing 10 nM DNR. At days 3, 10 and 14, 50 ml of fresh culture medium without DNR was added. At day 18 cells were pelleted and re-suspended in 160 ml medium containing 10 nM DNR. From day 26 to day 35 the DNR containing medium was changed once per week. At day 35 there were very few remaining viable cells, so cells were separated over Histopaque to exclude dead cells and cell debris. The remaining cells were re-suspended in 10 ml of medium containing 10 nM DNR up until day 45 when cell growth was seen. Cells were maintained at this drug concentration until their growth rate approached that of untreated OCI-AML3 cells. Over the next 10 weeks cells were exposed to gradually increasing concentrations of DNR up to a final concentration of 15 nM. By using the R123 accumulation assay these cells were confirmed to have increased levels of Pgp function and were subsequently cloned by limiting dilution. Cells were serially diluted in 96 well plates down to a concentration of 1 cell/well in medium containing 15 nM DNR. Any cells showing outgrowth were then gradually cultured in medium containing 15 nM DNR up to sufficient numbers to be able to assay for Pgp protein and function. One clone showed an increase in both Pgp protein expression and function. This clone was named OCI-AML3DNR and was then cultured under standard conditions with 15 nM DNR and also cryo-preserved for future use. DNR was removed from culture medium 7 days prior to any experimental procedure.
OCI-AML3/OCI-AML3DNR/OCI-AML6.2 genetic analysis
DNA was prepared using a QIAamp DNA blood mini kit (Qiagen, Crawley, UK) and 5 ng DNA was amplified using the Powerplex 16 System (Promega, Southampton, UK) to assess STR. The products were run on a 3130 Genetic Analyser and the data analysed using GeneMapper ID v3.2 software.
Patient samples
Presentation blood or bone marrow samples from multiple centres were obtained at diagnosis from patients with AML (excluding M3 subtype) and taken into preservative-free heparin or into EDTA tubes. All samples were pre-treatment and only samples received within 48 hours of being removed from the patient were analyzed. Use of these samples was approved by the Nottingham 1 Research Ethics Committee (reference number 06/Q2403/16). Mononuclear cells were isolated using a standard density gradient/centrifugation method with Histopaque and cryopreserved in liquid nitrogen. For analysis, cryopreserved samples were thawed and rested in culture medium enriched to 20% FCS for 90 minutes before experimental procedures. Thawed and rested samples were then subjected to viability analysis using trypan blue exclusion and only samples with > 85% post-rest viability were used.
Pgp protein expression - MRK-16 mAb
For Pgp protein expression cells were harvested, washed in PBSAA, and 2 × 10
5 cells incubated with 1 μg MRK-16 anti-Pgp monoclonal antibody (Kamiya Biomedical) or IgG2a isotype control (Dako) for 30 min at RT. Cells were washed x3 in PBSAA and blocked in 80 μl 20% normal rabbit serum for 30 min on ice. 5 μl FITC-conjugated goat anti-mouse secondary antibody (Dako) was added and cells incubated for 30 min on ice. Cells were washed twice in PBSAA and cell line data collected using a FACS calibur. Patient samples had the added step of the addition of 5 μl CD45PerCP (Becton Dickinson, UK) and 5 μl Normal Mouse serum before being washed twice in PBSAA and collected using a FACS calibur. Labelling the patient samples with CD45PerCP allowed the leukemic (CD45 low/side scatter low) cells to be gated [
53].
Determination of Pgp, BCRP and MRP function
Functional Pgp expression was determined by modulation of R123 efflux. Cells were resuspended at 1 × 10
6/ml in culture medium and incubated (75 minutes, 37°c) with R123 (200 ng/ml) with or without 2.5 μg/ml CSA (supplied by the pharmacy of Nottingham City Hospital) or diluent control. Control tubes containing R123 were placed on ice to halt the reaction [
53,
54]. Functional BCRP expression was measured similarly and was determined by modulation of BODIPY (Invitrogen) efflux. Cells were resuspended at 1 × 10
6/ml in culture medium and incubated (75 minutes, 37°c) with BODIPY (25 nM) with or without 10 μM FTC (Calbiochem) or diluent control. Control tubes containing BODIPY were placed on ice. MRP function was determined by modulation of Calcein-AM (Invitrogen) efflux [
55]. Cells were resuspended at 1 × 10
6/ml in culture medium and incubated (75 minutes, 37°c) with Calcein-AM (0.1 μM) with or without 40 μM MK-571 (Enzo life sciences, UK) or diluent control. Control tubes containing Calcein-AM were placed on ice. All tests were carried out in duplicate. Cells were washed twice in PBSAA at 4°c, collected using a FACS calibur (Becton Dickinson) and analysed using Cellquest software. Mean fluorescence intensity (MFI) of each sample in the FL1 channel was measured, since this corresponds to R123/BODIPY retention. The geometric mean fluorescence modulation ratio is calculated as: (MFI with modulator - cold control MFI)/(MFI with diluent control - cold control MFI) [
53,
54].
Real-time PCR for ABCG2 message levels
Thawed and rested AML blasts were depleted of CD2
+ cells using Dynabeads (to deplete NK and T cells some of which express ABCG2 mRNA [
56]. RNA from CD2 depleted AML blasts and the ABCG2 positive OCI-AML6.2 cell line was prepared using QIAamp RNA kits with DNase treatment according to the manufacturer's instructions (Qiagen). Quantitative PCR was carried out as previously reported [
22]. The relative expression levels of ABCG2 transcripts were calculated as the ratio between the level of ABCG2 and the level of β2M. Both sets of real time primers used have previously been published [
57,
58].
Determination of cell viability
This was done by a previously published in-house method which makes use of an internal standard and allows rapid enumeration of viable cells by flow cytometric counting of cells labelled with 7-amino Actinomycin D (7-AAD) [
59].
Histone H3 phosphorylation status
This was done by flow cytometry using anti-phospho-histone H3 (Ser 10) mouse monoclonal antibody (Upstate; now part of Millipore, Livingstone, UK) as previously described [
22]. 37 primary AML samples were cultured at 10
6/ml in RPMI 1640 with 10% FCS, 2 mM L-glutamine, supplemented with 20 ng/ml interleukin (IL)-3, 20 ng/ml stem cell factor, 20 ng/ml IL-6 + 25 ng/ml granulocyte colony-stimulating factor (R+D Systems) + 0.07 μl/ml beta-mercaptoethanol for 48 hours. Samples were then treated with 300 nM barasertib-hQPA for 60 minutes followed by pHH3 measurement by flow cytometry. pHH3 expression was calculated as a percentage of untreated controls.
UIC2 shift assay
The reactivity of the UIC2 antibody with Pgp is increased by the addition of Pgp transported compounds and has been described by Park [
44]. Briefly, cells were resuspended at 5 × 10
5/ml in culture medium and incubated for 10 minutes at 37°C, 5% C0
2. 1 μM barasertib-hQPA or 5 μM of the known Pgp substrate vinblastine, or both was added to cells in duplicate and incubated at 37°C, 5% C0
2 for 4 hours. Either 0.1 μg/ml UIC2 monoclonal antibody (Immunotech) or IgG2a isotype control (Dako) was added to paired samples and incubated for a further 15 minutes. Cells were washed twice in PBSAA, resuspended in 20% Rabbit serum, and placed on ice for 30 minutes. 5 μl FITC-conjugated rabbit anti-mouse secondary antibody (Dako) was then added to cells, and following 30 minutes incubation on ice, cells were washed twice and analysed by flow cytometry. An increase in FL1 fluorescence compared to a paired control is taken to indicate the presence of a Pgp substrate.
Radio-labelled drug accumulation assay
Pgp expressing OCI-AML3DNR cells and the Pgp null OCI-AML3 parent cell line were plated in duplicate onto 12 well plates and pre-incubated for 30 mins with or without the Pgp modulator CSA at 2.5 μg/ml. The BRCP expressing OCI-AML6.2 cells were treated similarly using BCRP null OCI-AML3 cells as a control and 10 μM FTC as the BCRP modulator. Medium containing 0.017 μCi radio-labelled [14C]-barasertib-hQPA (AstraZeneca, Cheshire, UK) was then added to give a final drug concentration of 300 nM. The cultures were incubated for 1 Hr at 37°c with agitation after 30 mins. The radioactive medium was then aspirated and cells washed twice with ice cold PBS before permeabilisation with 1% sodium dodecylsulphate (Sigma). Cell extracts were then added to scintillation fluid (Perkin Elmer) and radioactive drug retention measured using a TRI-CARB 2100TR liquid scintillation analyser (Packard).
Statistical Analysis
Statistical analysis was carried out using the Statistical Package for Social Sciences, version 16 (SPSS, Chicago, IL, USA). P values of less than or equal to 0.05 were considered to represent significance.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MG carried out all cell line and primary sample experiments and drafted the manuscript. MG and CS performed the RT PCR for ABCG2 message levels. CS performed the cell line genetic analysis. MP and NR conceived of the study. MP participated in the design and coordination of the study and helped to draft the manuscript. All authors critically read the manuscript and approved the final version.