Positive geotactic behaviors induced by geomagnetic field in Drosophila

To determine if GMF influences the geotactic behaviors of Drosophila, we constructed a GMF condition (henceforth, referred to as GMF, unless otherwise indicated) ranging from near-zero to ca. 85 μT which is comparable to the ambient GMF on Earth using a Helmholtz coil system (Fig. 1a). First, we attempted to modulate the intensity of GMF to a near-zero condition (Additional file 1: Table S1), and the tube-positioning assay, a modified version of the tube-climbing assay, was performed in the test cube to measure vertical positioning of flies at Zeitgeber time (ZT) 5 to ZT8 (Fig. 1b and Additional file 2: Figure S1A). A near-zero GMF condition was achieved by cancellation of all three GMF axes, X, Y, and Z, which significantly decreased the geotactic positioning scores (8 %–16 %) in all tested fly strains, Canton-S, white-eyed Canton-S, w ¹¹¹⁸ , Oregon-R, and Berlin-K (P < 0.01–0.001), compared to the sham condition (~45 μT in the lab) (Fig. 1c, Additional files 3 and 4) (By the definition described in Methods and Fig. 1b, a decrease in the geotactic positioning score indicates an increase in negative geotaxis and vice versa.). This potentiated negative geotaxis of flies under the near-zero GMF was similarly reproduced at ZT0–ZT2 (Additional file 2: Figure S1B), a circadian period characterized by higher locomotor activities in flies [19]. In addition, the geotactic responses modulated by countervailing fields in Helmholtz coil system was reproduced by passive cancellation of GMF with a permalloy cube made of nickel-iron alloy, a widely used metal for GMF shielding (Additional file 2: Figure S1C, S1D). In a time-course analysis, the geotactic response of Canton-S was fast (approximately 1 min, P < 0.005) and was sustained up to 11 min (P < 0.001) under the near-zero GMF (Fig. 1d and Additional file 4). Based on these robust and consistent responses, the Canton-S line was primarily used in subsequent experiments.

In another widely used geotaxis assay employing a vertical choice Y-maze [4] (Fig. 1e), more flies left the maze through the upper exits (1 and 3) than the lower exits (4–6) under the near-zero GMF than the ambient GMF (P < 0.05) (Fig. 1f), confirming that attenuation of GMF strengthens the negative geotactic nature of flies. As a control, the exit profiles of flies through the vertical maze versus horizontal maze in the ambient GMF were reproduced as previously described [4] (Additional file 2: Figure S1E). To further confirm the near-zero GMF-induced negative geotaxis in a more natural setup, a free-flight assay in which flies could freely fly in a cubic arena was also conducted (Fig. 1g). Consistent with the above results, the near-zero GMF elicited flying at higher elevations (P < 0.05) (Fig. 1h). Collectively, these results strongly suggest that GMF antagonizes negative geotaxis in Drosophila.

A growing body of evidence suggests that the individual components of GMF provide fundamentally different information to magnetosensitive animals. The vector of GMF provides directional information, whereas the intensity and/or inclination provide positional information for horizontal movement [15]. However, GMF has not been previously suggested as a cue for vertical movement of animals such as geotactic behaviors. Since the near-zero GMF potentiated negative geotaxis as shown in Fig. 1, we next asked whether any GMF conditions with increased intensity make flies actively move downward to the bottom per se. Therefore, we examined the geotactic behaviors of flies in response to specific GMF conditions with increased intensity up to 85 μT (Additional file 5: Table S2). Interestingly, we observed robust positive geotactic responses of the flies under the GMF conditions at 71 μT (b in Fig. 2a) and 85 μT (c in Fig. 2a) (P < 0.005 and < 0.001, respectively). To rule out possible influence from the experimental setup (see Fig. 1b, left), we carried out a control experiment by rotating the test cube 90° counterclockwise in the horizontal plane. The geotactic scores were comparable between the two directions of the test cube under the near-zero GMF and the positive geotactic GMF condition b (Additional file 6: Figure S2A). These results demonstrate that flies can move downward, i.e., positive geotactic, under the GMF conditions with increased intensity.

It has been known that magnetosensitive movements in Drosophila are mediated by a blue light-dependent manner [20, 21]. We evaluated the possibility that wild-type flies exhibit differential geotactic behaviors in response to light and dark conditions. Interestingly, flies did not exhibit substantial changes in geotactic behaviors under the near-zero GMF and the positive geotactic GMF condition b in Fig. 2a in the dark condition (0 lx) (Fig. 2b and c), indicating that light is required for GMF-modulated geotaxis. In addition, the potentiated negative geotaxis at short wavelengths (400–420 nm) (P < 0.005) was comparable to that at full spectrum (P < 0.005) (Additional file 6: Figure S2B), demonstrating that blue light is sufficient for the GMF-modulated geotaxis.

Next, we examined whether the GMF-modulated positive geotaxis is biologically relevant for a stereotyped fly behavior. To address this question, we conducted an associative learning experiment in which the fly memory on the food located at the bottom of a test tube was associated with the memory on a non-geotactic GMF condition (Fig. 2d). The non-geotactic GMF condition a was demonstrated to function as a neutral stimulus for geotactic responses (Fig. 2a). If starved flies successfully learned to associate the appetite-driven stimulus with the non-geotactic GMF, the flies would exhibit a positive geotactic response upon the sole presentation of the non-geotactic GMF without food. To associate the appetite-driven stimulus with the non-geotactic GMF condition, a group of starved flies was initially trained for 2 min under non-geotactic GMF conditions with food. After a 2 min-rest with the test tube upside-down to eliminate any potential bias for the bottom of the tube, the starved flies were subjected to the non-geotactic GMF condition without food for the test. As a control, parallel experiments were performed in which no food was supplied during the initial training (Fig. 2d). Strikingly, the flies trained by the non-geotactic GMF stimulus with food (Fig. 2e, right) exhibited significant positive geotactic responses under the non-geotactic GMF alone (P < 0.005) (Fig. 2e, right), but no remarkable behavioral changes under the sham condition (Fig. 2e, right). By contrast, the control flies for which no food was associated with the non-geotactic GMF stimulus (Fig. 2e, middle) did not exhibit a noticeable positive geotactic response under the non-geotactic GMF condition (Fig. 2e, middle). Collectively, these results demonstrate that GMF can function as a biologically relevant behavioral cue for vertical movement in flies, particularly when it is associated with the appetitive stimulus that is compulsory for survival in the wild.

To characterize the biological mechanisms underlying the GMF-modulated positive geotaxis, we evaluated the requirements of the signaling pathways that convey the environmental magnetoreceptive information toward the locomotive organs. cry was identified as a geotaxis gene through behavioral genetic screening, and an inverse correlation between the cry transcript level and negative geotaxis was observed [3]. In addition, given the observed results in Fig. 2b, c and Additional file 6: Figure S2B and that CRY is a light-responsive protein [20, 21], we hypothesized that CRY is involved in the GMF-induced geotaxis. To test this hypothesis, CRY-deficient cry ⁰¹ mutant flies [20] backcrossed into the wild-type Canton-S background were used to enable the comparison of their geotactic behaviors with those of Canton-S flies as controls. First, we observed that CRY-deficient flies displayed abrogated negative geotactic behaviors that were normally potentiated by the near-zero GMF (Fig. 3a). To check the positive geotactic responses of the mutants, we used the same GMF condition b (Intensity = 71 μT) that elicited positive geotactic responses in Fig. 2a. In this condition, CRY-deficient flies exhibited impaired positive geotactic responses compared to wild-type controls, and CRY expression-rescued flies exhibited normal positive geotactic responses (Fig. 3b and c, respectively). In addition, RNAi knockdown of cry using the cry-GAL4 driver completely abolished the positive geotactic responses compared to wild-type and other control flies (Fig. 3d). Moreover, CRY-deficient flies were defective in the near-zero GMF-induced negative geotaxis and the CRY expression-rescued flies recovered the geotactic behavioral phenotype (Additional file 7: Figure S3A). Similarly, the impaired geotactic response in cry-knockdown flies was restored by the recovering of the expression of CRY in the mutant flies (Additional file 7: Figure S3B).

Previous studies suggested that pdf and pdf receptor (pdfr) play crucial roles in geotaxis [3, 22]. Thus, we examined whether PDF also plays a role in the GMF-modulated geotactic behaviors using pdf ⁰¹ mutant flies, a PDF-deficient line [23]. Interestingly, pdf ⁰¹ mutant flies were defective in both the near-zero GMF-induced negative geotactic and positive geotactic responses compared to wild-type controls (Fig. 3e and f). Genetic restoration of PDF expression in the mutants fully rescued the defect in both geotactic responses (Fig. 3g and Additional file 7: Figure S3C). Supporting these results, specific knockdown of pdf using pdf-GAL4 driver also impaired both the near-zero GMF-induced negative geotaxis and positive geotactic responses (Fig. 3h and Additional file 7: Figure S3D). Together, these results demonstrate that PDF is required for the GMF-modulated geotactic behavior in flies.

We next examined the GMF-modulated geotactic responses in a pyx-deficient mutant line because Pyx encoded by pyx belongs to the TRP channel family and critically contributes to gravity sensing in the Drosophila JO [7, 8]. The mutant flies exhibited impaired GMF-modulated positive geotaxis and near-zero GMF-induced negative geotaxis (Fig. 3i and j, respectively), and both of the impaired geotactic responses were successfully recovered by genetic rescue of pyx (Fig. 3k and Additional file 7: Figure S3E, respectively). Similarly, RNAi knockdown of pyx abrogated the positive geotactic positioning and near-zero GMF-induced negative geotaxis (Fig. 3l and Additional file 7: Figure S3F, respectively). These results consistently suggest that Pyx is required for the GMF-modulated geotactic behaviors.

Having shown that CRY, PDF and Pyx genes are required for the GMF-modulated geotactic behaviors, we investigated the neuroanatomical loci critical for the behaviors. We paid first attention to JO that is comprised of specialized neurons and cap cells for perceiving mechanical stimuli and sensing gravity for normal geotaxis (Fig. 4a) [7, 8]. Intriguingly, Pyx is expressed in the cap cells in JO [8] and a batch of prominent CRY neurons morphologically corresponding to JO neurons was visualized (Fig. 4b). However, we found no evidence for PDF neurons located in JO (Fig. 4c). Prior to testing the necessity of these neurons in the GMF-modulated geotaxis, we confirmed whether JO is indeed critical for the GMF-modulated geotactic responses. To achieve this, we physically injured JO in the second segment of antenna [8] and checked the geotactic responses. As a result, we observed that the flies with injured JO exhibited severely impaired GMF-modulated geotactic behaviors (Fig. 4d). As a comparison, we also cut off flies’ wings [24] and examined the injured flies’ geotactic behaviors, but found no significant difference between the injured flies and controls (Additional file 8: Figure S4A), substantiating the hypothesis that JO is critical locus for the GMF-modulated geotaxis in flies. To next examine if the CRY, PDF and Pyx neurons in JO are required for the GMF-modulated geotactic responses, we targeted expression of tetanus toxin (TNT) to silence each of these neurons expressing CRY, PDF and Pyx using their specific GAL4 drivers. Interestingly, silencing CRY, PDF and Pyx neurons all abrogated the GMF-modulated positive geotaxis (Fig. 4e-g). Also, silencing these neurons abolished the near-zero GMF-induced negative geotaxis (Additional file 8: Figure S4B-S4D). Taken together, these results demonstrate that a neural circuit expressing CRY, PDF, and Pyx is required for the GMF-modulated geotactic behaviors and suggest that the circuit-mediated sensory processes for the GMF-modulated geotactic responses may occur in JO.

To determine whether the functions of CRY, PDF and Pyx in JO are critical for the GMF-modulated geotactic responses, we selectively restored the gene expression of cry, pdf and pyx in the fly mutants of these genes using two JO-specific GAL4 drivers, nanchung (nan) and inactive (iav) [8]. Supporting the hypothesis, JO-specific expression of exogenous cry using these two different GAL4 drivers in CRY-deficient flies completely rescued the impaired GMF-modulated geotactic behaviors (Fig. 5a, b, Additional file 9: Figure S5A and S5B). Similarly, the defective GMF-modulated geotactic responses in Pyx-deficient flies were also rescued by JO-specific expression of exogenous pyx (Fig. 5c, d, Additional file 9: Figure S5C and S5D). These results indicate that CRY and Pyx function in JO to mediate the GMF-modulated geotactic behaviors.

Strikingly, however, JO-specific expression of PDF was not sufficient to rescue the defective GMF-modulated geotactic responses of pdf mutant flies (Fig. 5e, f, Additional file 9: Figure S5E and S5F). This result is in consistence with the notion that PDF is not expressed in JO (Fig. 4c), suggesting that the PDF neurons in the remaining body parts are responsible for the GMF-modulated geotactic responses. Intriguingly, we found that RNAi knockdown of pdf in CRY- or Pyx-expressing cells impaired the GMF-modulated geotactic responses (Fig. 5g, h, Additional file 9: Figure S5G and S5H). Taken together, these data suggest that PDF functions in the GMF-modulated geotactic responses elsewhere in the CRY- and Pyx-expressing cells.

All flies were reared with standard cornmeal-yeast-agar diet at 25 °C, 60 % relative humidity, in a 12 h light/12 h dark cycle under a full-spectrum fluorescent light that was turned on at 09:00 (local time), and the ambient geomagnetic field (GMF; total intensity = 44.9 μT, X (North–south) = 32.2 μT, Y (East–west) = −5.8 μT, Z (vertical to ground) = 30.8 μT). Fly strains of Canton-S, Oregon-R, w ¹¹¹⁸, Berlin-K, cry-GAL4 ¹⁶ , nan-GAL4, iav-GAL4, UAS-mCD8::GFP, UAS-cry RNAi (BL25859), and UAS-pyx RNAi (BL31297) were provided by the Bloomington Drosophila Stock Center (Indiana University, Bloomington, IN, USA). UAS-pdf RNAi (v4380) was provided by Vienna Drosophila RNAi Center (Vienna, Austria). Other flies were gifts: cry ⁰¹ flies [20] from Dr. S. M. Reppert (Univ. of Massachusetts, USA); pdf ⁰¹ [23] from Dr. J. H. Park (Univ. of Tennessee, USA); UAS-TNT and UAS-ImpTNT [32] from Dr. C. J. O’Kane (Univ. of Cambridge, UK), and pdf-GAL4 [19] from Dr. J. Choe (KAIST, Korea); pyx-GAL4 [8] from Dr. F. N. Hamada (Cincinnati Children’s Hospital Medical Center, USA); UAS-cry [33] from Dr. E. Rosato (Univ. of Leicester, UK); UAS-pdf [19] from Dr. P. H. Taghert (Washington Univ., USA). cry ⁰¹ , pdf ⁰¹ , and pyx ³ were outcrossed to Canton-S background for 6–8 generations. For restoring the mutant phenotype using GAL4-UAS system in each cry ⁰¹ , pdf ⁰¹ , or pyx ³ mutant background, pyx-GAL4 and iav-GAL4 were recombined onto the third chromosome carrying each mutant allele. There was no abnormal phenotype in appearances and life cycle during the development of fly strains throughout the experiments, except low oviposition rate in PDF-deficient files.

A rectangular Helmholtz coil system (Fig. 1a) consisting of three pairs of parallel coils arranged orthogonally for three axes was constructed based on our previous Helmholtz coil system [34‐36], and it was used to modulate the intensity of three GMF vectors by active cancellation. The coil for X axis (North–south) was aligned with true geographic north so that Y-coil has a room for contributing to modulate Y vector of GMF. The position of geographic north was determined using the local declination value (−7.53 at the time of set-up) of Daegu city outside the building where the experiments were performed. Using this set-up, we intended to maximize the diversity of GMF conditions close to the real world for testing flies’ geotactic behaviors. Average dimensions of the coils were 1,890 × 1,890 mm, 1,890 × 1,800 mm, and 1,980 × 1,980 mm, for the X, Y, and Z axes, respectively. Coils for each axis comprised two bundles (1 mm in diameter enameled Cu wire, 175-turns/bundle) on an open non-magnetic aluminum frame aligned in parallel with each other, with effective distances of 1,029 mm, 980 mm, and 1,078 mm for the X, Y, and Z axes, respectively. A pair of coils for each axis was connected to an adjustable DC power supply (E3631A; Agilent Technologies, Santa Clara, CA, USA). GMF was measured using a 3-axis gaussmeter (MGM 3AXIS; ALPHALAB, West Salt Lake City, UT, USA), and homogeneity of the GMF in the sample space was measured as 99 %, 95 %, and 90 % for the tube-positioning assay, Y-maze assay, and free-flight assay, respectively. The modified GMF parameter conditions and the intensity of fluorescent light (500 lx unless otherwise mentioned) were indicated in figure legends or table captions accordingly. The ambient power frequency of the 60-Hz magnetic field was less than 3 μT, as measured by a gaussmeter (TES 1390; TES Electrical Electronic, Taipei, Taiwan). Throughout the experiments, 60 Hz electric field across the assay area was measured using (3D NF Analyzer NFA 1000; Gigahertz Solutions, Fürth, Bayern, Germany). The difference of electric field between experimental conditions was negligible; sham (1.22 V/m), cancellation of X, Y, and Z (1.21 V/m), condition a (1.21 V/m), b (1.22 V/m), and c (1.22 V/m).

To decrease GMF to near zero (indicated as – in figures), we cancelled the intensity of each axis of the ambient GMF by countervailing the each axis using the coil system (Figs. 1c, d, f, h, 2b, 3a, e, i, 4d, Additional file 2: Figure S1B, Additional file 6: Figure S2A-B, Additional file 7: Figure S3A-F, Additional file 8: Figure S4A-D and Additional file 9: Figure S5A-H). Conversely, positive geotaxis was induced by strengthening the total intensity as appeared in b and c of Fig. 2a. The strengthening condition was indicated as + in Figs. 2c, 3b-d, f-h, j-l, 4d-g, 5a-h and Additional file 8: Figure S4A.

Exceptionally, passive shielding was used for Additional file 2: Figure S1D. This shielding condition was achieved by conducting behavioral experiments in a double-layered permalloy (0.5-mm thick) cube (180 × 100 × 140 mm, length × width × height) (Additional file 2: Figure S1C) with one open-side for handling of samples and recording of flight behaviors. The passive shielding experiment also showed similarly potentiated negative geotaxis comparable to the countervailing method using the coil system (Additional file 2: Figure S1D). The cancellation efficiency for total intensity was ca. 36-fold (69-, 71-, and 33-fold for the X, Y, and Z axes, respectively), and the homogeneity was about 95 % in the sample space.

All the experiments using the coil were conducted in a temperature-controlled room kept at 25 °C and the temperature across the assaying area was monitored using thermometers (USB Datalogger 98581; MIC Meter Industrial Company, Taichung City, Taiwan). The fly samples were set inside the coils 3 s after turning on the current. The tube-positioning assay was performed mostly between ZT5 and ZT8 as well as ZT0 and ZT2 in a separate experiment according to a previous study [37] with some modifications. Prior to the assay, flies (1 ~ 3-day-old) taken from the rearing incubator were put into a pooled flask (180 mL) and accommodated for 10 min. The flies (45 ± 2) were then transferred into a transparent polypropylene test tube (20 × 850 mm, diameter × height) sealed by a cotton plug at the orifice without anesthesia. The test tubes with the flies were kept inverted on the top for 1 min and then gently placed with the bottom down inside either a cube (180 × 100 × 140 mm, length × width × height) (Fig. 1b and Additional file 2: Figure S1A) located at the center of the Helmholtz coil. Vertical positioning of the flies in the test tubes was video-recorded for 11 min and quantified as the “geotactic positioning score” using the following equation: (number of flies at the lowest four sections of the test tube that was equally divided into five imaginary sections/total number of flies) × 100 % (see Fig. 1b). Other alternative equations that included ‘the lowest three sections’ and ‘the lowest two sections’ instead of ‘the lowest four sections’ showed similar scores (Pearson correlation [R] values were 0.98 and 0.96, respectively). The calculation was performed on the five consecutive captured photos at 5 s interval, and the average of the scores from the five photos was used as a data point.

For the removal of the JO or wings in Fig. 4d and Additional file 8: Figure S4A, the JO was injured by pinching the second antennal segments with fine forceps [8] or wings were cut off at the hinge region with micro-scissors [24] under anesthesia with CO₂ [8, 24]. The injured flies were accommodated to the rearing incubator for 24 h before the tube-positioning assay (10 flies/test), and then the assay was performed.

The Y-maze assay was adopted as an alternative geotaxis assay with some modifications [3, 4]. The six-exit maze was constructed from clear polypropylene T- and Y-shaped connectors 4 mm in diameter (Kartell, Noviglio, Italy), and a 4-mm-bore non-toxic silicon tube (Cole-Parmer, Vernon Hills, IL, USA) (Fig. 1e). For collection of flies at the exits, a semi-transparent 1-ml plastic tip used for micropipettes was connected to each exit. Each tip was jointed with a tapered 15-ml clear polypropylene conical tube to prevent flies from reentering the maze through the exit. The prepared maze was fixed on a transparent acryl plate for rigidity and placed at the center of the Helmholtz coils. The Y-maze assay was performed between ZT0 and ZT2. For each experiment, 25 ± 2 flies were transferred from a pooled flask to a 15-ml clear polypropylene conical tube (feeding tube) and allowed to enter the maze through a 10-cm-long silicon tube linking the feeding tube and the entrance of the maze. The flies were kept there for 30 min until at least 90 % of them were collected. Light was placed parallel to all exits, and the intensity of light was 350 lx and 500 lx at the entrance and all exits, respectively. The exit score for each exit was calculated as (number of flies collected at each exit/total number of flies collected at all exits) × 100 %. Exit profiles were compared between the sham (the ambient GMF) and near-zero GMF conditions (total intensity, 0.015 ± 0.006 μT), in which the plane of the maze was vertical to the ground. As a control, the exit profile of the sham-horizontal maze was compared to that of the sham-vertical maze.

The free-flight assay was performed to determine the effect of GMF-induced modulation on free flying behavior of flies in a cubic arena (0.6 × 0.6 × 0.6 m) (Fig. 1g). The five planes of the arena were made of pale white board, whereas the front plane was a transparent film through which light could shine and flying behavior could be recorded. A fluorescent light lamp was placed 5 cm above the bottom line of the arena, and the light intensity was 500 lx at the point of departure. The assay was performed between ZT0 and ZT2. For each experiment, 30 ± 2 flies were transferred from the pooled flask to a 15-ml clear polypropylene conical tube. Upon removing the cap, the tube was instantly intruded 6 cm into the arena through a hole (20 mm in diameter) at the rear-center region of the bottom, and the flies were allowed to crawl up to the orifice and fly freely. Flying behavior was recorded for 2 min. The GMF inside of the arena was modulated as indicated in Fig. 1h. Flying score was calculated as (number of flies flown onto the upper section of the front plane/total number of flies flown onto the whole section of the front plane) × 100 %. As a control, the flying profile under the sham conditions (the ambient GMF) was determined.

The associative learning assay was carried out according to the scheme depicted in Fig. 2d. One to two-day-old Canton-S flies were transferred to an empty flask containing Whatman paper soaked with distilled water and starved for 24 h; they were then placed in another empty flask containing no water for 6 h before training. In each experiment, 20 ± 2 flies were used. For the training group, flies were loaded into a training tube containing food, the same rearing diet, either in the sham or the non-geotactic GMF for 2 min. The trained flies were transferred to a test tube that was inverted during the 2-min rest and subsequently tested for 2 min. In the control experiments, flies were processed by the same procedure without food in a tube. For the test, the tube-positioning assay was conducted to measure geotactic behavior of the trained or control flies under the sham or the non-geotactic GMF condition. The trained and control flies were experimented consecutively. The non-geotactic GMF was generated using a home-made one-axis square Helmholtz coil (250 × 250 × 100 mm, 1,000 turns, 1 mm in diameter enameled Cu wire) on open acrylic frame. The coils were positioned perpendicular to a test tube at its base; homogeneity of the non-geotactic GMF intensity at the lower one-third of test tube was 95 % (see Fig. 2d).

Statistical analyses were performed by Student’s t-test or one-way analysis of variance (ANOVA) Tukey’s test using Origin software (San Clemente, CA, USA). Statistical values are presented as mean ± standard error of the mean (SEM). In all analyses, P < 0.05 is regarded as significant. All experiments were repeated at least 10 times.

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Supporting data are found in the supporting materials; 5 supporting figures, 2 supporting tables, 2 supporting videos, and one striking image.