Erschienen in:
23.12.2017 | Original Article
Prevalence of Listeria monocytogenes in cerebrospinal fluid obtained from hospitalized patients
verfasst von:
Tayebeh Zeinali, Abdollah Jamshidi, Kiarash Ghazvini, Mohammadreza Bassami, Mehrnaz Rad
Erschienen in:
Comparative Clinical Pathology
|
Ausgabe 2/2018
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Abstract
The aim of the present study was to determine the occurrence of Listeria monocytogenes in cerebrospinal fluid (CSF) samples obtained from patients with meningitis symptoms. A total of 102 CSF samples which were referred for bacterial culture to microbiology laboratory in Mashhad’s Hospital were included in this study. All of the samples were cultured and positive ones confirmed by multiplex-PCR. Molecular serotyping and virulence characterization of isolates were performed. Random amplified polymorphic DNA-PCR using three set of primers was performed for evaluation of genetic diversity of these isolates. Five out of 102 samples were positive for L. monocytogenes. Three different serotypes were detected, and all of the isolates were potentially pathogenic. Low genetic diversity was determined among isolated L. monocytogenes.