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Digestive Diseases and Sciences

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01.03.2007 | Original Article

Probiotic Effects on 5-Fluorouracil-Induced Mucositis Assessed by the Sucrose Breath Test in Rats

verfasst von: Chad A. Mauger, Ross N. Butler, Mark S. Geier, Katie L. Tooley, Gordon S. Howarth

Erschienen in: Digestive Diseases and Sciences | Ausgabe 3/2007

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Abstract

The sucrose breath test (SBT) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-FU)-induced intestinal mucositis. Dark Agouti rats were allocated to 5 groups (n=10): 5-FU+L. fermentum BR11, 5-FU+L. rhamnosus GG, 5-FU+B. lactis BB12, 5-FU+skim milk (SM), and saline+SM. Probiotics were administered by oral gavage for 10 days. Mucositis was induced on day 7 by intraperitoneal injection of 5-FU (150 mg/kg) or vehicle (saline). Rats were sacrificed 72 h after 5-FU injection. The SBT measured breath ¹³CO₂ (expressed as percentage cumulative dose at 90 min; %CD90) on days 0, 7, and 10. %CD90 was significantly lower in 5-FU-treated controls compared with that in saline-treated controls on day 10. 5-FU caused an 83% reduction in sucrase and a 510% increase in MPO activity. The SBT detected damage induced by 5-FU and is a simple, noninvasive indicator of small bowel injury. The probiotics assessed offered no protection from mucositis at the dose tested.

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Titel: Probiotic Effects on 5-Fluorouracil-Induced Mucositis Assessed by the Sucrose Breath Test in Rats
verfasst von: Chad A. Mauger
Ross N. Butler
Mark S. Geier
Katie L. Tooley
Gordon S. Howarth
Publikationsdatum: 01.03.2007
Verlag: Kluwer Academic Publishers-Plenum Publishers
Erschienen in: Digestive Diseases and Sciences / Ausgabe 3/2007
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9464-y

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