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Experimental Brain Research

Erschienen in:

01.01.2007 | Research Article

Prostaglandin E₂ potentiates the excitability of small diameter trigeminal root ganglion neurons projecting onto the superficial layer of the cervical dorsal horn in rats

verfasst von: Jun Kadoi, M. Takeda, S. Matsumoto

Erschienen in: Experimental Brain Research | Ausgabe 2/2007

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Abstract

The aim of the present study was to investigate how prostaglandin E₂ (PGE₂) affects the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the superficial layer of the cervical dorsal horn, using fluorescence retrograde tracing and perforated patch-clamp techniques. TRG neurons were retrogradely labeled with fluorogold (FG). The cell diameter of FG-labeled neurons was small (< 30 μm). Under the voltage-clamp mode, application of PGE₂ (0.01–10 μM) concentration-dependently increased the magnitude of the peak tetrodotoxin-resistant sodium current (TTX-R I _Na) and this current was maximal at a concentration of 1 μM. One micromolar PGE₂ application caused a hyperpolarizing shift of 8.3 mV in the activation curve for TTX-R I _Na. In the current-clamp mode, the PGE₂ (1 μM) application significantly increased the number of action potentials during the depolarizing step pulses as well as the level of overshoot but had no significant effect on the resting membrane potential. These results suggest that the excitability of small diameter TRG neurons seen after 1 μM PGE₂ application is involved in an increase in the TTX-R I _Na.

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Titel: Prostaglandin E2 potentiates the excitability of small diameter trigeminal root ganglion neurons projecting onto the superficial layer of the cervical dorsal horn in rats
verfasst von: Jun Kadoi
M. Takeda
S. Matsumoto
Publikationsdatum: 01.01.2007
Verlag: Springer-Verlag
Erschienen in: Experimental Brain Research / Ausgabe 2/2007
Print ISSN: 0014-4819
Elektronische ISSN: 1432-1106
DOI: https://doi.org/10.1007/s00221-006-0608-2

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