nach oben

Erschienen in:

01.09.2011 | Original Research Paper

Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells

verfasst von: Masanobu Takao, Toshinori Okinaga, Wataru Ariyoshi, Kenjiro Iwanaga, Ikuo Nakamichi, Izumi Yoshioka, Kazuhiro Tominaga, Tatsuji Nishihara

Erschienen in: Inflammation Research | Ausgabe 9/2011

Einloggen, um Zugang zu erhalten

Abstract

Objective

The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells.

Materials and methods

HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E₂ (PGE₂) released into the culture medium were determined using ELISA.

Results

Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE₂ synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE₂ synthesis induced by mechanical stretch.

Conclusion

We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation.

Honsho S, Nishikawa S, Amano K, Zen K, Adachi Y, Kishita E, Matsui A, Katsume A, Yamaguchi S, Nishikawa K, Isoda K, Riches DW, Matoba S, Okigaki M, Matsubara H. Pressure-mediated hypertrophy and mechanical stretch induces IL-1 release and subsequent IGF-1 generation to maintain compensative hypertrophy by affecting Akt and JNK pathways. Circ Res. 2009;105:1149–58.PubMedCrossRef

Inoh H, Ishiguro N, Sawazaki S, Amma H, Miyazu M, Iwata H, Sokabe M, Naruse K. Uni-axial cyclic stretch induces the activation of transcription factor nuclear factor kappaB in human fibroblast cells. FASEB J. 2002;16:405–7.PubMed

Milam SB, Schmitz JP. Molecular biology of temporomandibular joint disorders: proposed mechanisms of disease. J Oral Maxillofac Surg. 1995;53:1448–54.PubMedCrossRef

Van Kujik AW, Reinders-Blanket P, Smeets TJ, Dijkmans BA, Tak PP. Detailed analysis of the cell infiltrate and the expression of mediators of synovial inflammation and joint destruction in the synovium of patients with psoriatic arthritis: implications for treatment. Ann Rheum Dis. 2006;65:1551–7.CrossRef

Ritchlin C, Haas-Smith SA, Hicks D, Cappuccio J, Osterland CK, Looney RJ. Patterns of cytokine production in psoriatic synovium. J Rheumatol. 1998;25:1544–52.PubMed

Quinn JH, Bazan NG. Identification of prostaglandin E2 and leukotriene B4 in the synovial fluid of painful, dysfunctional temporomandibular joints. J Oral Maxillofac Surg. 1990;48:968–71.PubMedCrossRef

Mohan AR, Sooranna SR, Lindstrom TM, Johnson MR, Bennett PR. The effect of mechanical stretch on cyclooxygenase type 2 expression and activator protein-1 and nuclear factor-kappaB activity in human amnion cells. Endocrinology. 2007;148:1850–7.PubMedCrossRef

Smith WL, DeWitt DL, Garavito RM. Cyclooxygenases: structural, cellular, and molecular biology. Annu Rev Biochem. 2000;69:145–82.PubMedCrossRef

Williams CS, Mann M, DuBois RN. The role of cyclooxygenases in inflammation, cancer, and development. Oncogene. 1999;18:7908–16.PubMedCrossRef

10.

Cao Y, Prescott SM. Many actions of cyclooxygenase-2 in cellular dynamics and in cancer. J Cell Physiol. 2002;190:279–86.PubMedCrossRef

11.

Dinarello CA. Biology of interleukin 1. FASEB J. 1988;2:108–15.PubMed

12.

Maines MD. Heme oxygenase: function, multiplicity, regulatory mechanism, and clinical applications. FASEB J. 1988;2:2557–68.PubMed

13.

Vijayan V, Mueller S, Baumgart-Vogt E, Immenschuh S. Heme oxygenase-1 as a therapeutic target in inflammatory disorders of gastrointestinal tract. World J Gastroenterol. 2010;16:3112–9.PubMedCrossRef

14.

Immenschuh S, Ramandori G. Gene regulation of heme oxygenase-1 as a therapeutic target. Biochem Pharmacol. 2000;60:1121–8.PubMedCrossRef

15.

Willis D, Moore AR, Fredrick R, Willoughby DA. Heme oxygenase: a novel target for the modulation of the inflammatory response. Nat Med. 1996;2:87–90.PubMedCrossRef

16.

Wagner CT, Durante W, Christodoulides N, Hellums JD, Schafer AI. Hemodynamic forces induce the expression of heme oxygenase in cultured vascular smooth muscle cells. J Clin Invest. 1997;100:589–96.PubMedCrossRef

17.

Yachie A, Niida Y, Wada T, Igarashi N, Kaneda H, Toma T, Ohta K, Kasahara Y, Koizumi S. Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency. J Clin Invest. 1999;103:129–35.PubMedCrossRef

18.

Jeong GS, Lee DS, Kim YC. Cudratricusxanthone A from Cudrania tricuspidata suppresses pro-inflammatory mediators through expression of anti-inflammatory heme oxygenase-1 in RAW264.7 macrophages. Int Immunopharmacol. 2009;9:241–6.PubMedCrossRef

19.

Pi SH, Jeong GS, Oh HW, Kim YS, Pae HO, Chung HT, Lee SK, Kim EC. Heme oxygenase-1 mediates nicotine- and lipopolysaccharide-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in human periodontal ligament cells. J Periodontal Res. 2010;45:177–83.PubMedCrossRef

20.

Toshinaga A, Hosokawa R, Okinaga T, Masaki C, Tsujisawa T, Nishihara T. Inflammatory response in epithelial cells induced by mechanical stress is suppressed by hyaluronic acid. Inflamm Regen. 2010;30:120–7.CrossRef

21.

Ichimiya H, Takahashi T, Ariyoshi W, Takano H, Matayoshi T, Nishihara T. Compressive mechanical stress promotes osteoclast formation through RANKL expression on synovial cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:334–41.PubMedCrossRef

22.

Tanne K. Degenerative changes of articular cartilage in association with mechanical stimuli. Jpn Dent Sci Rev. 2008;44:38–47.CrossRef

23.

Kubota E, Imamura H, Kubota T, Shibata T, Murakami K. Interleukin 1 beta and stromelysin (MMP3) activity of synovial fluid as possible markers of osteoarthritis in the temporomandibular joint. J Oral Maxillofac Surg. 1997;55:27–8.CrossRef

24.

Fan Z, Söder S, Oehler S, Fundel K, Aigner T. Activation of interleukin-1 signaling cascades in normal and osteoarthritis articular cartilage. Am J Pathol. 2007;171:938–46.PubMedCrossRef

25.

Huang J, Ballou LR, Hasty KA. Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Gene. 2007;404:101–9.PubMedCrossRef

26.

Satoh K, Akutsu M, Kuboyama N, Kuyama K, Yamamoto H, Kondoh T. Expression of cyclooxygenase-1 and -2 in IL-1β-induced synovitis of the temporomandibular joint. J Oral Pathol Med. 2009;38:584–90.PubMedCrossRef

27.

Morisugi T, Tanaka Y, Kawakami T, Kirita T. Mechanical stretch enhances NF-kappaB-dependent gene expression and poly (ADP-ribose) synthesis in synovial cells. J Biochem. 2010;147:633–44.PubMedCrossRef

28.

Vandenburgh HH, Hatfaludy S, Sohar I, Shansky J. Stretch-induced prostaglandins and protein turnover in cultured skeletal muscle. Am J Physiol. 1990;259:232–40.

29.

Vandenburgh HH, Shansky J, Solerssi R, Chromiak J. Mechanical stimulation of skeletal muscle increases prostaglandin F2 alpha production, cyclooxygenase activity, and cell growth by a pertussis toxin sensitive mechanism. J Cell Physiol. 1995;163:285–94.PubMedCrossRef

30.

Keyse SM, Tyrrell RM. Heme oxygenase is the major 32 kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite. Proc Natl Acad Sci USA. 1989;86:99–103.PubMedCrossRef

31.

Taketani S, Kohno H, Yoshinaga T, Tokunaga R. The human 32 kda stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase. FEBS Lett. 1989;245:173–6.PubMedCrossRef

32.

Vercellotti GM, Balla G, Balla J, Nath K, Eaton JW, Jacob HS. Heme and the vasculature: an oxidative hazard that induces antioxidant defenses in the endothelium. Artif Cells Blood Substit Immobil Biotechnol. 1994;22:207–13.PubMedCrossRef

33.

Balla J, Jacob HS, Balla G, Nath K, Vercellotti GM. Endothelial cell heme oxygenase and ferritin induction by heme proteins: a possible mechanism limiting shock damage. Trans Assoc Am Physicians. 1992;105:1–6.PubMed

34.

Morse D, Pischke SE, Zhou Z, Davis RJ, Flavell RA, Loop T, Otterbein SL, Otterbein LE, Choi AM. Suppression of inflammatory cytokine production by carbon monoxide involves the JNK pathway and AP-1. J Biol Chem. 2003;278:36993–8.PubMedCrossRef

35.

Lee TS, Tsai HL, Chau LY. Induction of heme oxygenase-1 expression in murine macrophages is essential for the anti-inflammatory effect of low dose 15-deoxy-Delta 12, 14-prostaglandin J2. J Biol Chem. 2003;278:19325–30.PubMedCrossRef

36.

Suh GY, Jin Y, Yi AK, Wang XM, Choi AM. CCAT/enhancer-binding protein mediates carbon monoxide-induced suppression of cyclooxygenase-2. Am J Respir Cell Mol Biol. 2006;35:220–6.PubMedCrossRef

37.

Shin RH, Yang CM. Induction of heme oxygenase-1 attenuates lipopolysaccharide-induced cyclooxygenase-2 expression in mouse brain endothelial cells. J Neuroinflammation. 2010;7:86.CrossRef

Titel: Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells
verfasst von: Masanobu Takao
Toshinori Okinaga
Wataru Ariyoshi
Kenjiro Iwanaga
Ikuo Nakamichi
Izumi Yoshioka
Kazuhiro Tominaga
Tatsuji Nishihara
Publikationsdatum: 01.09.2011
Verlag: SP Birkhäuser Verlag Basel
Erschienen in: Inflammation Research / Ausgabe 9/2011
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-011-0346-1

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells