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10.01.2022 | Original Article

Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

verfasst von: Talita Mendes Oliveira Ventura, Karina Oliveira Santos, Aline Silva Braga, Larissa Tercilia Grizzo Thomassian, Marília Afonso Rabelo Buzalaf, Fábio Augusto Barbieri, Carlos Augusto Kalva-Filho, Murilo Henrique Faria, Ana Carolina Magalhães

Erschienen in: Sport Sciences for Health | Ausgabe 3/2022

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Abstract

The practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity.

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Titel: Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results
verfasst von: Talita Mendes Oliveira Ventura
Karina Oliveira Santos
Aline Silva Braga
Larissa Tercilia Grizzo Thomassian
Marília Afonso Rabelo Buzalaf
Fábio Augusto Barbieri
Carlos Augusto Kalva-Filho
Murilo Henrique Faria
Ana Carolina Magalhães
Publikationsdatum: 10.01.2022
Verlag: Springer Milan
Erschienen in: Sport Sciences for Health / Ausgabe 3/2022
Print ISSN: 1824-7490
Elektronische ISSN: 1825-1234
DOI: https://doi.org/10.1007/s11332-021-00883-z

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Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

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