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10.01.2022 | Original Article

Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

verfasst von: Talita Mendes Oliveira Ventura, Karina Oliveira Santos, Aline Silva Braga, Larissa Tercilia Grizzo Thomassian, Marília Afonso Rabelo Buzalaf, Fábio Augusto Barbieri, Carlos Augusto Kalva-Filho, Murilo Henrique Faria, Ana Carolina Magalhães

Erschienen in: Sport Sciences for Health | Ausgabe 3/2022

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Abstract

The practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity.

Nuzum H, Stickel A, Corona M, Zeller M, Melrose RJ, Wilkins SS (2020) Potential benefits of physical activity in MCI and dementia. Behav Neurol. https://doi.org/10.1155/2020/7807856CrossRefPubMedPubMedCentral

Warburton DER, Bredin SSD (2017) Health benefits of physical activity: a systematic review of current systematic reviews. Curr Opin Cardiol 32(5):541–556. https://doi.org/10.1097/HCO.0000000000000437CrossRefPubMed

Finkler M, Lichtenberg D, Pinchuk I (2014) The relationship between oxidative stress and exercise. J Basic Clin Physiol Pharmacol 25(1):1–11. https://doi.org/10.1515/jbcpp-2013-0082CrossRefPubMed

Hajizadeh Maleki B, Tartibian B, Mooren FC, FitzGerald LZ, Kruger K, Chehrazi M, Malandish A (2018) Low-to-moderate intensity aerobic exercise training modulates irritable bowel syndrome through antioxidative and inflammatory mechanisms in women: results of a randomized controlled trial. Cytokine 102:18–25. https://doi.org/10.1016/j.cyto.2017.12.016CrossRefPubMed

Shi M, Wang X, Yamanaka T, Ogita F, Nakatani K, Takeuchi T (2007) Effects of anaerobic exercise and aerobic exercise on biomarkers of oxidative stress. Environ Health Prev Med 12(5):202–208. https://doi.org/10.1265/ehpm.12.202CrossRefPubMedPubMedCentral

Souza AV, Giolo JS, Teixeira RR, Vilela DD, Peixoto LG, Justino AB, Caixeta DC, Puga GM, Espindola FS (2019) Salivary and plasmatic antioxidant profile following continuous, resistance, and high-intensity interval exercise: preliminary study. Oxid Med Cell Longev 2019:5425021. https://doi.org/10.1155/2019/5425021CrossRefPubMedPubMedCentral

Malamud D (2011) Saliva as a diagnostic fluid. Dent Clin N Am 55(1):159–178. https://doi.org/10.1016/j.cden.2010.08.004CrossRefPubMed

Proctor GB, Carpenter GH (2007) Regulation of salivary gland function by autonomic nerves. Auton Neurosci 133(1):3–18. https://doi.org/10.1016/j.autneu.2006.10.006CrossRefPubMed

Bosch JA, Veerman EC, de Geus EJ, Proctor GB (2011) Alpha-amylase as a reliable and convenient measure of sympathetic activity: don’t start salivating just yet! Psychoneuroendocrinology 36(4):449–453. https://doi.org/10.1016/j.psyneuen.2010.12.019CrossRefPubMed

10.

Ligtenberg AJ, Brand HS, van den Keijbus PA, Veerman EC (2015) The effect of physical exercise on salivary secretion of MUC5B, amylase and lysozyme. Arch Oral Biol 60(11):1639–1644. https://doi.org/10.1016/j.archoralbio.2015.07.012CrossRefPubMed

11.

de Oliveira VN, Bessa A, Lamounier RP, de Santana MG, de Mello MT, Espindola FS (2010) Changes in the salivary biomarkers induced by an effort test. Int J Sports Med 31(6):377–381. https://doi.org/10.1055/s-0030-1248332CrossRefPubMed

12.

Bortolini MJS, De Agostini GG, Reis IT, Lamounier RPMS, Blumberg JB, Espindola FS (2009) Total protein of whole saliva as a biomarker of anaerobic threshold. Res Q Exerc Sport 80(3):604–610CrossRef

13.

Ligtenberg AJ, Liem EH, Brand HS, Veerman EC (2016) The effect of exercise on salivary viscosity. Diagnostics (Basel). https://doi.org/10.3390/diagnostics6040040CrossRef

14.

Mulic A, Tveit AB, Songe D, Sivertsen H, Skaare AB (2012) Dental erosive wear and salivary flow rate in physically active young adults. BMC Oral Health 12:8. https://doi.org/10.1186/1472-6831-12-8CrossRefPubMedPubMedCentral

15.

Gillum T, Kuennen M, McKenna Z, Castillo M, Jordan-Patterson A, Bohnert C (2017) Exercise increases lactoferrin, but decreases lysozyme in salivary granulocytes. Eur J Appl Physiol 117(5):1047–1051. https://doi.org/10.1007/s00421-017-3594-0CrossRefPubMed

16.

Buzalaf MA, Hannas AR, Kato MT (2012) Saliva and dental erosion. J Appl Oral Sci 20(5):493–502. https://doi.org/10.1590/s1678-77572012000500001CrossRefPubMedPubMedCentral

17.

Kruger K, Mooren FC, Pilat C (2016) The immunomodulatory effects of physical activity. Curr Pharm Des 22(24):3730–3748. https://doi.org/10.2174/1381612822666160322145107CrossRefPubMed

18.

Simpson RJ, Kunz H, Agha N, Graff R (2015) Exercise and the regulation of immune functions. Prog Mol Biol Transl Sci 135:355–380. https://doi.org/10.1016/bs.pmbts.2015.08.001CrossRefPubMed

19.

Sari-Sarraf V, Reilly T, Doran DA, Atkinson G (2007) The effects of single and repeated bouts of soccer-specific exercise on salivary IgA. Arch Oral Biol 52(6):526–532. https://doi.org/10.1016/j.archoralbio.2006.11.016CrossRefPubMed

20.

Herman JP, Cullinan WE (1997) Neurocircuitry of stress: central control of the hypothalamo-pituitary–adrenocortical axis. Trends Neurosci 20(2):78–84. https://doi.org/10.1016/s0166-2236(96)10069-2CrossRefPubMed

21.

Maruyama Y, Kawano A, Okamoto S, Ando T, Ishitobi Y, Tanaka Y, Inoue A, Imanaga J, Kanehisa M, Higuma H, Ninomiya T, Tsuru J, Hanada H, Akiyoshi J (2012) Differences in salivary alpha-amylase and cortisol responsiveness following exposure to electrical stimulation versus the Trier Social Stress Tests. PLoS ONE 7(7):e39375. https://doi.org/10.1371/journal.pone.0039375CrossRefPubMedPubMedCentral

22.

de Vries WR, Bernards NT, de Rooij MH, Koppeschaar HP (2000) Dynamic exercise discloses different time-related responses in stress hormones. Psychosom Med 62(6):866–872. https://doi.org/10.1097/00006842-200011000-00017CrossRefPubMed

23.

Leicht CA, Goosey-Tolfrey VL, Bishop NC (2018) Exercise intensity and its impact on relationships between salivary immunoglobulin A, saliva flow rate and plasma cortisol concentration. Eur J Appl Physiol 118(6):1179–1187. https://doi.org/10.1007/s00421-018-3847-6CrossRefPubMedPubMedCentral

24.

Rutherfurd-Markwick K, Starck C, Dulson DK, Ali A (2017) Salivary diagnostic markers in males and females during rest and exercise. J Int Soc Sports Nutr 14:27. https://doi.org/10.1186/s12970-017-0185-8CrossRefPubMedPubMedCentral

25.

Ventura T, Cassiano LPS, Souza ESCM, Taira EA, Leite AL, Rios D, Buzalaf MAR (2017) The proteomic profile of the acquired enamel pellicle according to its location in the dental arches. Arch Oral Biol 79:20–29. https://doi.org/10.1016/j.archoralbio.2017.03.001CrossRefPubMed

26.

Jasim H, Olausson P, Hedenberg-Magnusson B, Ernberg M, Ghafouri B (2016) The proteomic profile of whole and glandular saliva in healthy pain-free subjects. Sci Rep 6:39073. https://doi.org/10.1038/srep39073CrossRefPubMedPubMedCentral

27.

Ventura T, Ribeiro NR, Dionizio AS, Sabino IT, Buzalaf MAR (2018) Standardization of a protocol for shotgun proteomic analysis of saliva. J Appl Oral Sci 26:e20170561. https://doi.org/10.1590/1678-7757-2017-0561CrossRefPubMedPubMedCentral

28.

Winck FV, Prado Ribeiro AC, Ramos Domingues R, Ling LY, Riano-Pachon DM, Rivera C, Brandao TB, Gouvea AF, Santos-Silva AR, Coletta RD, Paes Leme AF (2015) Insights into immune responses in oral cancer through proteomic analysis of saliva and salivary extracellular vesicles. Sci Rep 5:16305. https://doi.org/10.1038/srep16305CrossRefPubMedPubMedCentral

29.

Buzalaf MAR, Ortiz AC, Carvalho TS, Fideles SOM, Araujo TT, Moraes SM, Buzalaf NR, Reis FN (2020) Saliva as a diagnostic tool for dental caries, periodontal disease and cancer: is there a need for more biomarkers? Expert Rev Mol Diagn 20(5):543–555. https://doi.org/10.1080/14737159.2020.1743686CrossRefPubMed

30.

Wang X, Kaczor-Urbanowicz KE, Wong DT (2017) Salivary biomarkers in cancer detection. Med Oncol 34(1):7. https://doi.org/10.1007/s12032-016-0863-4CrossRefPubMed

31.

Ruegsegger GN, Booth FW (2018) Health benefits of exercise. Cold Spring Harb Perspect Med. https://doi.org/10.1101/cshperspect.a029694CrossRefPubMedPubMedCentral

32.

Gaudet P, Livstone MS, Lewis SE, Thomas PD (2011) Phylogenetic-based propagation of functional annotations within the gene ontology consortium. Brief Bioinform 12(5):449–462. https://doi.org/10.1093/bib/bbr042CrossRefPubMedPubMedCentral

33.

Simpson RJ, Lowder TW, Spielmann G, Bigley AB, LaVoy EC, Kunz H (2012) Exercise and the aging immune system. Ageing Res Rev 11(3):404–420. https://doi.org/10.1016/j.arr.2012.03.003CrossRefPubMed

34.

Romeo J, Warnberg J, Pozo T, Marcos A (2010) Physical activity, immunity and infection. Proc Nutr Soc 69(3):390–399. https://doi.org/10.1017/S0029665110001795CrossRefPubMed

35.

Delecrode TR, Siqueira WL, Zaidan FC, Bellini MR, Moffa EB, Mussi MC, Xiao Y, Buzalaf MA (2015) Identification of acid-resistant proteins in acquired enamel pellicle. J Dent 43(12):1470–1475. https://doi.org/10.1016/j.jdent.2015.10.009CrossRefPubMed

36.

Crosara KTB, Zuanazzi D, Moffa EB, Xiao Y, Machado M, Siqueira WL (2018) Revealing the amylase interactome in whole saliva using proteomic approaches. Biomed Res Int 2018:6346954. https://doi.org/10.1155/2018/6346954CrossRefPubMedPubMedCentral

37.

UniProt C (2021) UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Res 49(D1):D480–D489. https://doi.org/10.1093/nar/gkaa1100CrossRef

38.

Kunz H, Bishop NC, Spielmann G, Pistillo M, Reed J, Ograjsek T, Park Y, Mehta SK, Pierson DL, Simpson RJ (2015) Fitness level impacts salivary antimicrobial protein responses to a single bout of cycling exercise. Eur J Appl Physiol 115(5):1015–1027. https://doi.org/10.1007/s00421-014-3082-8CrossRefPubMed

39.

Jendzjowsky NG, DeLorey DS (2012) Short-term exercise training augments sympathetic vasoconstrictor responsiveness and endothelium-dependent vasodilation in resting skeletal muscle. Am J Physiol Regul Integr Comp Physiol 303(3):R332-339. https://doi.org/10.1152/ajpregu.00053.2012CrossRefPubMed

40.

Just TP, DeLorey DS (2016) Exercise training and alpha1-adrenoreceptor-mediated sympathetic vasoconstriction in resting and contracting skeletal muscle. Physiol Rep 4(3):1. https://doi.org/10.14814/phy2.12707CrossRef

41.

Pagan LU, Gomes MJ, Okoshi MP (2018) Endothelial function and physical exercise. Arq Bras Cardiol 111(4):540–541. https://doi.org/10.5935/abc.20180211CrossRefPubMedPubMedCentral

42.

Silva-Boghossian CM, Colombo AP, Tanaka M, Rayo C, Xiao Y, Siqueira WL (2013) Quantitative proteomic analysis of gingival crevicular fluid in different periodontal conditions. PLoS ONE 8(10):e75898. https://doi.org/10.1371/journal.pone.0075898CrossRefPubMedPubMedCentral

43.

Vukosavljevic D, Custodio W, Buzalaf MA, Hara AT, Siqueira WL (2014) Acquired pellicle as a modulator for dental erosion. Arch Oral Biol 59(6):631–638. https://doi.org/10.1016/j.archoralbio.2014.02.002CrossRefPubMed

44.

Crewther BT, Lowe TE, Ingram J, Weatherby RP (2010) Validating the salivary testosterone and cortisol concentration measures in response to short high-intensity exercise. J Sports Med Phys Fit 50(1):85–92

45.

Noland RC (2015) Exercise and regulation of lipid metabolism. Prog Mol Biol Transl Sci 135:39–74. https://doi.org/10.1016/bs.pmbts.2015.06.017CrossRefPubMed

46.

Tosheska Trajkovska K, Topuzovska S (2017) High-density lipoprotein metabolism and reverse cholesterol transport: strategies for raising HDL cholesterol. Anatol J Cardiol 18(2):149–154. https://doi.org/10.14744/AnatolJCardiol.2017.7608CrossRefPubMed

47.

Wilund KR, Colvin PL, Phares D, Goldberg AP, Hagberg JM (2002) The effect of endurance exercise training on plasma lipoprotein AI and lipoprotein AI:AII concentrations in sedentary adults. Metabolism 51(8):1053–1060. https://doi.org/10.1053/meta.2002.33356CrossRefPubMed

48.

Padiglia A, Orru R, Boroumand M, Olianas A, Manconi B, Sanna MT, Desiderio C, Iavarone F, Liori B, Messana I, Castagnola M, Cabras T (2018) Extensive characterization of the human salivary basic proline-rich protein family by top-down mass spectrometry. J Proteome Res 17(9):3292–3307. https://doi.org/10.1021/acs.jproteome.8b00444CrossRefPubMed

49.

Proctor GB (2016) The physiology of salivary secretion. Periodontol 2000 70(1):11–25. https://doi.org/10.1111/prd.12116CrossRefPubMed

50.

Chojnowska S, Ptaszynska-Sarosiek I, Kepka A, Knas M, Waszkiewicz N (2021) Salivary biomarkers of stress, anxiety and depression. J Clin Med. https://doi.org/10.3390/jcm10030517CrossRefPubMedPubMedCentral

51.

Feeney EL, Leacy L, O’Kelly M, Leacy N, Phelan A, Crowley L, Stynes E, de Casanove A, Horner K (2019) Sweet and umami taste perception differs with habitual exercise in males. Nutrients. https://doi.org/10.3390/nu11010155CrossRefPubMedPubMedCentral

52.

Gauthier AC, Guimaraes RF, Namiranian K, Drapeau V, Mathieu ME (2020) Effect of physical exercise on taste perceptions: a systematic review. Nutrients. https://doi.org/10.3390/nu12092741CrossRefPubMedPubMedCentral

53.

Humphrey SP, Williamson RT (2001) A review of saliva: normal composition, flow, and function. J Prosthet Dent 85(2):162–169. https://doi.org/10.1067/mpr.2001.113778CrossRefPubMed

54.

Matsuo R, Garrett JR, Proctor GB, Carpenter GH (2000) Reflex secretion of proteins into submandibular saliva in conscious rats, before and after preganglionic sympathectomy. J Physiol 527(Pt 1):175–184. https://doi.org/10.1111/j.1469-7793.2000.00175.xCrossRefPubMedPubMedCentral

Titel: Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results
verfasst von: Talita Mendes Oliveira Ventura
Karina Oliveira Santos
Aline Silva Braga
Larissa Tercilia Grizzo Thomassian
Marília Afonso Rabelo Buzalaf
Fábio Augusto Barbieri
Carlos Augusto Kalva-Filho
Murilo Henrique Faria
Ana Carolina Magalhães
Publikationsdatum: 10.01.2022
Verlag: Springer Milan
Erschienen in: Sport Sciences for Health / Ausgabe 3/2022
Print ISSN: 1824-7490
Elektronische ISSN: 1825-1234
DOI: https://doi.org/10.1007/s11332-021-00883-z

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Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

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