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Archives of Virology

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01.08.2012 | Brief Report

Sequencing and analysis of the complete genome of Rana grylio virus (RGV)

verfasst von: Xiao-Ying Lei, Tong Ou, Ruo-Lin Zhu, Qi-Ya Zhang

Erschienen in: Archives of Virology | Ausgabe 8/2012

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Abstract

Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.

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Titel: Sequencing and analysis of the complete genome of Rana grylio virus (RGV)
verfasst von: Xiao-Ying Lei
Tong Ou
Ruo-Lin Zhu
Qi-Ya Zhang
Publikationsdatum: 01.08.2012
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 8/2012
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-012-1316-9

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