nach oben

Erschienen in:

01.09.2014 | Original Contribution

Short-term creatine supplementation does not reduce increased homocysteine concentration induced by acute exercise in humans

verfasst von: Rafael Deminice, Flávia Troncon Rosa, Gabriel Silveira Franco, Selma Freirede Carvalho da Cunha, Ellen Cristini de Freitas, Alceu Afonso Jordao

Erschienen in: European Journal of Nutrition | Ausgabe 6/2014

Einloggen, um Zugang zu erhalten

Abstract

Purpose

The purpose of the study is to evaluate the effects of creatine supplementation on homocysteine (Hcy) plasma levels after acute exercise in humans.

Methods

Twenty-three young (under-20) soccer players were divided into 2 groups: creatine (Cr)- and placebo (Pla)-supplemented groups. The supplementation was performed in double-blind controlled manner using creatine or placebo tablets with 0.3 g/kg during 7 days. Before and after 7 days of supplementation, the athletes performed an acute high-intensity sprint exercise (two consecutive running-based anaerobic sprint test protocol consisted in 6 × 35 m sprint with 10 s between them). Blood samples were collected before and after 7 days of supplementation as well as 0 and 1 h after exercise protocol.

Results

Homocysteine concentration significant increased (P < 0.05) 1 h after acute exercise (18 %). Acute exercise also decreased red blood cell S-adenosylmethionine (SAM) 30 % with no changes in SAM/SAH ratio. Seven days of creatine supplementation were able to increase (P < 0.05) plasma creatine concentration (Pla 130.1 ± 21.7 vs Cr 1,557.2 ± 220.3 μmol/L) as well as decrease (P < 0.05) plasma guanidinoacetic acid (33 %). Controversially, creatine supplementation did not change Hcy plasma level after 7-day supplementation (Pla 6.9 ± 0.2 vs Cr 7.2 ± 0.2 μmol/L) or after acute exercise (Pla 8.2 ± 0.3 vs Cr 8.4 ± 0.3 μmol/L). No changes in plasma vitamin B12 and folate as well as cysteine and methionine were found.

Conclusions

Seven days of creatine supplementation does not avoid increased plasma Hcy induced by acute sprint exercise in humans.

Steed MM, Tyagi SC (2011) Mechanisms of cardiovascular remodeling in hyperhomocysteinemia. Antioxid Redox Signal 1(15):1927–1943. doi:10.1089/ars2010.3721 CrossRef

Taes YE, Delanghe JR, De Bacquer D, Langlois M, Stevens L, Geerolf I, Lameire NH, De Vriese AS (2004) Creatine supplementation does not decrease total plasma homocysteine in chronic hemodialysis patients. Kidney Int 66:2422–2428. doi:10.1111/j.1523-1755.2004.66019.x CrossRef

Wijekoon EP, Brosnan ME, Brosnan JT (2007) Homocysteine metabolism in diabetes. Biochem Soc Trans 35:1175–1179CrossRef

Chen CS, Yeh YC, Chang YS, Huang MF (2011) Plasma homocysteine level and apathy in Alzheimer’s disease. J Am Geriatr Soc 59(9):1752–1754. doi:10.1111/j.1532-5415.2011.03550.x CrossRef

Deminice R, da Silva RP, Lamarre SG, Brown C, Furey GN, McCarter SA, Jordao AA, Kelly KB, King-Jones K, Jacobs RL, Brosnan ME, Brosnan JT (2011) Creatine supplementation prevents the accumulation of fat in the livers of rats fed a high-fat diet. J Nutr 141(10):1799–1804. doi:10.3945/jn.111.144857 CrossRef

Brosnan JT, Jacobs RL, Stead LM et al (2004) Methylation demand: a key determinant of homocysteine metabolism. Act Biol Pol 51:405–413

Brosnan JT, da Silva R, Brosnan ME (2007) Amino acids and the regulation of methyl balance in humans. Curr Opin Clin Nutr Metab Care 10:52–57. doi:10.1097/MCO.0b013e3280110171 CrossRef

Selhub J (1999) Homocysteine metabolism. Annu Rev Nutr 19:217–246CrossRef

Brosnan JT, da Silva RP, Brosnan ME (2011) The metabolic burden of creatine synthesis. Amino Acids 40:1325–1331. doi:10.1007/s00726-011-0853-y CrossRef

10.

Stead LM, Brosnan JT, Brosnan ME, Vance DE, Jacobs RL (2006) Is it time to revaluate methyl balance in humans? Am J Clin Nutr 83:5–10

11.

Edison EE, Brosnan ME, Meyer C, Brosnan JT (2007) Creatine synthesis: production of guanidinoacetate by the rat and human kidney in vivo. Am J Physiol Renal Physiol 293:F1799–F1804. doi:10.1152/ajprenal.0 0356.2007

12.

da Silva RP, Nissim I, Brosnan ME, Brosnan JT (2009) Creatine synthesis: hepatic metabolism of guanidinoacetate and creatine in the rat in vitro and in vivo. Am J Physiol Endocrinol Metab 296(2):E256–E261. doi:10.1152/ajpendo.90547.2008 CrossRef

13.

Stead LM, Au KP, Jacobs RL, Brosnan ME, Brosnan JT (2001) Methylation demand and homocysteine metabolism: effects of dietary provision of creatine and guanidinoacetate. Am J Physiol Endocrinol Metab 281:E1095–E1100

14.

Taes YE, Delanghe JR, De Vriese AS, Rombait R, Vam Camp J, Lameire NH (2003) Creatine supplementation decrease homocysteine in an animal model of uremia. Kidney Int 64:1331–1337. doi:10.1046/j.1523-1755.2003.00206.x CrossRef

15.

Deminice R, Portari GV, Vannucchi H, Jordao AA (2009) Effects of creatine supplementation on homocysteine levels and lipid peroxidation in rats. Br J Nutr 102:110–116. doi:10.1017/S0007114508162985 CrossRef

16.

Deminice R, Vannucchi H, Simões-Ambrosio LM, Jordao AA (2011) Creatine supplementation reduces increased homocysteine concentration induced by acute exercise in rats. Eur J Appl Physiol 111:2663–2670. doi:10.1007/s00421-011-1891-6 CrossRef

17.

Herrmann M, Schorr H, Obeid R, Scharhag J, Urhausen A, Kindermann W, Herrmann W (2003) Homocysteine increases during endurance exercise. Clin Chem Lab Med 41:1518–1524. doi:10.1515/CCLM.2003.233

18.

König D, Bissé E, Deibert P, Müller HM, Wieland H, Berg A (2003) Influence of training volume and acute physical exercise on the homocysteine levels in endurance-trained men: interactions with plasma folate and vitamin B12. Ann Nutr Metab 47:114–118. doi:10.1159/000070032 CrossRef

19.

Venta R, Cruz E, Valcárcel G, Terrados N (2009) Plasma vitamins, amino acids, and renal function in postexercise hyperhomocysteinemia. Med Sci Sports Exerc 41:1645–1651. doi:10.1249/MSS.0b013e31819e02f2 CrossRef

20.

Hultman E, Söderlund K, Timmons JA, Cederblad G, Greenhaff PL (1996) Muscle creatine loading in men. J Appl Physiol 81:232–237

21.

Derave W, Marescau B, Vanden Eede E, Eijnde BO, De Deyn PP, Hespel P (2004) Plasma guanidino compounds are altered by oral creatine supplementation in healthy humans. J Appl Physiol 97(3):852–857. doi:10.1152/japplphysiol.00206.2004 CrossRef

22.

Costa CM, Santos RCC, Lima ES (2006) A simple automated procedure for thiol measurement in human serum samples. J Bras Patol Med Lab 42:345–350. doi:10.1590/S1676-24442006000500006 CrossRef

23.

Buchberger W, Ferdig M (2004) Improved high-performance liquid chromatographic determination of guanidino compounds by precolumn derivatization with ninhydrin and fluorescence detection. J Sep Sci 27:1309–1312CrossRef

24.

Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37(2):247–248

25.

American College of Sports Medicine position stand (2009) Nutrition and athletic performance. Med Sci Sports Exerc 41(3):709–731. doi:10.1249/MSS.0b013e318190eb86 CrossRef

26.

Korzun WJ (2004) Oral creatine supplements lower plasma homocysteine concentrations in humans. Clin Lab Sci 17(2):102–106

27.

Steenge GR, Verhoef P, Greenhaff PL (2001) The effect of creatine and resistance training on plasma homocysteine concentration in healthy volunteers. Arch Intern Med 161:1455–1456CrossRef

28.

Freilinger M, Dunkler D, Lanator I, Item CB, Mühl A, Fowler B, Bodamer OA (2011) Effects of creatine supplementation in Rett syndrome: a randomized, placebo-controlled trial. J Dev Behav Pediatr 32(6):454–460. doi:10.1097/DBP.0b013e31822177a8 CrossRef

29.

Stead LM, Brosnan ME, Brosnan JT (2000) Characterization of homocysteine metabolism in the rat liver. Biochem J 15(350 Pt 3):685–692CrossRef

30.

Ueland PM (1995) Homocysteine species as components of plasma redox thiol status. Clin Chem 41(3):340–342

31.

Brosna JT (2001) Homocysteine and the kidney. In: Carmel R, Jacobsen DW (eds) Homocysteine in health and disease. Cambridge University Press, Cambridge, pp 176–184

32.

Friedman AN, Bostom AG, Selhub J, Levey AS, Rosenberg IH (2001) The kidney and homocysteine metabolism. J Am Soc Nephrol 12(10):2181–2189

33.

Van Hall G, Saltin B, Wagenmakers AJ (1999) Muscle protein degradation and amino acid metabolism during prolonged knee-extensor exercise in humans. Clin Sci (Lond) 97(5):557–567CrossRef

34.

Rennie MJ, Tipton KD (2000) Protein and amino acid metabolism during and after exercise and the effects of nutrition. Annu Rev Nutr 20:457–483. doi:10.1146/annurev.nutr.20.1.457 CrossRef

35.

Joubert LM, Manore MM (2006) Exercise, nutrition, and homocysteine. Int J Sport Nutr Exerc Metab 16(4):341–361

36.

Sotgia S, Carru C, Caria MA, Tadolini B, Deiana L, Zinellu A (2007) Acute variations in homocysteine levels are related to creatine changes induced by physical activity. Clin Nutr 26:444–449. doi:10.1016/j.clnu.2007.05.003 CrossRef

Titel: Short-term creatine supplementation does not reduce increased homocysteine concentration induced by acute exercise in humans
verfasst von: Rafael Deminice
Flávia Troncon Rosa
Gabriel Silveira Franco
Selma Freirede Carvalho da Cunha
Ellen Cristini de Freitas
Alceu Afonso Jordao
Publikationsdatum: 01.09.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 6/2014
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-013-0636-1

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Reizdarmsyndrom: Diäten wirksamer als Medikamente

29.04.2024 Reizdarmsyndrom Nachrichten

Bei Reizdarmsyndrom scheinen Diäten, wie etwa die FODMAP-arme oder die kohlenhydratreduzierte Ernährung, effektiver als eine medikamentöse Therapie zu sein. Das hat eine Studie aus Schweden ergeben, die die drei Therapieoptionen im direkten Vergleich analysierte.

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2014

Dietary factors associated with metabolic risk score in Finnish children aged 6–8 years: the PANIC study

Hepatic inflammation induced by high-fructose diet is associated with altered 11βHSD1 expression in the liver of Wistar rats

Green tea catechins and blood pressure: a systematic review and meta-analysis of randomised controlled trials

Repeated versus single measurement of plasma omega-3 fatty acids and risk of heart failure

Frequencies and demographic determinants of breastfeeding and DHA supplementation in a nationwide sample of mothers in Germany