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Forensic Toxicology

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24.10.2016 | Short Communication

Simple protein precipitation-based analysis of Δ⁹-tetrahydrocannabinol and its metabolites in human serum by liquid chromatography–tandem mass spectrometry

verfasst von: Marek Dziadosz, Michael Klintschar, Jörg Teske

Erschienen in: Forensic Toxicology | Ausgabe 1/2017

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Abstract

A liquid chromatography–tandem mass spectrometry (LC–MS/MS) quantification method based on a direct protein precipitation with the mobile phase was developed, validated and applied for the analysis of Δ⁹-tetrahydrocannabinol (THC), 11-hydroxy-Δ⁹-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THC-COOH) in human serum for forensic purposes. Chromatographic separation was performed with a Luna 5 µm C18 (2) 100 A, 150 × 2 mm analytical column and the elution with a programmed flow of a mobile phase consisting of A (H₂O/methanol, 95:5, v/v) and B (H₂O/methanol, 3:97, v/v), both with 10 mM ammonium acetate and 0.1 % acetic acid. The results of the validation experiments demonstrated that the strategy presented can be applied successfully for a fast, simple and cost effective LC–MS/MS analysis of THC and its metabolites. A potential conversion of THC-COOH glucuronide to THC-COOH during sample preparation/analysis could be excluded.

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Titel: Simple protein precipitation-based analysis of Δ9-tetrahydrocannabinol and its metabolites in human serum by liquid chromatography–tandem mass spectrometry
verfasst von: Marek Dziadosz
Michael Klintschar
Jörg Teske
Publikationsdatum: 24.10.2016
Verlag: Springer Japan
Erschienen in: Forensic Toxicology / Ausgabe 1/2017
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-016-0344-7

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Simple protein precipitation-based analysis of Δ⁹-tetrahydrocannabinol and its metabolites in human serum by liquid chromatography–tandem mass spectrometry

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

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