nach oben

Erschienen in:

01.04.2016 | Original Article

Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry

verfasst von: Hideki Nozawa, Kayoko Minakata, Itaru Yamagishi, Koutaro Hasegawa, Masako Suzuki, Kunio Gonmori, Osamu Suzuki, Kanako Watanabe

Erschienen in: Forensic Toxicology | Ausgabe 2/2016

Einloggen, um Zugang zu erhalten

Abstract

A rapid and sensitive method using matrix-assisted laser desorption ionization (MALDI)-quadrupole time-of-flight (QTOF)-mass spectrometry (MS) was developed for the analysis of six tricyclic antidepressants (ADs) and their related drugs, such as amitriptyline, carbamazepine, clomipramine, imipramine, nortriptyline, quetiapine, and two tetracyclic ADs, mianserin and mirtazapine, because these eight drugs are commonly observed medicines in poisoning cases in Japan. In the present MALDI-MS method, the limits of detection were 0.3–2 ng/mL. The present method provided a simple and high throughput simultaneous analysis of these drugs. This is the first report dealing with quantitation of ADs and their related drugs in biological samples by MALDI-MS. In addition, we applied the MALDI-MS to two authentic cases, and three new metabolites of amitriptyline and three new metabolites of quetiapine could be detected in blood and urine samples in the two cases when regioisomers were counted as one metabolite.

Kudo K, Ishida T, Hikiji W, Usumoto Y, Umehara T, Nagamatsu K, Tsuji A, Ikeda N (2010) Pattern of poisoning in Japan: selection of drugs and poisons for systematic toxicological analysis. Forensic Toxicol 28:25–32CrossRef

Hayashi D, Kumazawa T, Hasegawa C, Lee X-P, Marumo A, Uchigasaki S, Kawamura M, Sato K (2012) A simple and reliable method for quantifying plasma concentrations of tetracyclic antidepressants using monolithic silica solid-phase extraction tips. Forensic Toxicol 30:98–105CrossRef

Papoutsis I, Khraiwesh A, Nikolaou P, Pistos C, Spiliopoulou C, Athanaselis S (2012) A fully validated method for the simultaneous determination of 11 antidepressant drugs in whole blood by gas chromatography-mass spectrometry. J Pharm Biomed Anal 70:557–562CrossRefPubMed

Pietracci E, Bermejo A-M, Álvarez I, Cabarcos P, Balduini W, Tabernero M-J (2013) Simultaneous determination of new-generation antidepressants in plasma by gas chromatography-mass spectrometry. Forensic Toxicol 31:124–132CrossRef

Breaud AR, Harlan R, Kozak M, Clarke W (2009) A rapid and reliable method for the quantitation of tricyclic antidepressants in serum using HPLC-MS/MS. Clin Biochem 42:1300–1307CrossRefPubMed

Breaud AR, Harlan R, Di Bussolo JM, McMillin GA, Clarke W (2010) A rapid and fully-automated method for the quantitation of tricyclic antidepressants in serum using turbulent-flow liquid chromatography-tandem mass spectrometry. Clin Chim Acta 411:825–832CrossRefPubMed

Hasselstrøm J (2011) Quantification of antidepressants and antipsychotics in human serum by precipitation and ultra high pressure liquid chromatography-tandem mass spectrometry. J Chromatogr B 879:123–128CrossRef

Amundsen I, Øiestad ÅML, Ekeberg D, Kristoffersen L (2013) Quantitative determination of fifteen basic pharmaceuticals in ante- and post-mortem whole blood by high pH mobile phase reversed phase ultra high performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 927:112–123CrossRef

Yonezawa T, Asano T, Fujino T, Nishihara H (2013) Cyclodextrin-supported organic matrix for application of MALDI-MS for forensics. Soft-ionization to obtain protonated molecules of low molecular weight compounds. Chem Phys 419:17–22CrossRef

10.

Nozawa H, Minakata K, Yamagishi I, Hasegawa K, Wurita A, Gonmori K, Suzuki O, Watanabe K (2015) MALDI-TOF mass spectrometric determination of eight benzodiazepines with two of their metabolites in blood. Leg Med 17:150–156CrossRef

11.

Guo Z, Zhang Q, Zou H, Guo B, Ni J (2003) A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry. Anal Chem 74:1637–1641CrossRef

12.

Schulz M, Iwersen-Bergmann S, Andresen H, Schmoldt A (2012) Therapeutic and toxic blood concentrations of nearly 1000 drugs and other xenobiotics. Crit Care 16(4):R136. doi:10.1186/cc11441 CrossRefPubMedPubMedCentral

13.

Ulrich S, Isensee T, Pester U (1996) Simultaneous determination of amitriptyline, nortriptyline and four hydroxylated metabolites in serum by capillary gas-liquid chromatography with nitrogen-phosphorus-selective detection. J Chromatogr B 685:81–89CrossRef

14.

Broecker S, Pragst F, Bakdash A, Herre S, Tsokos M (2011) Combined use of liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) and high performance liquid chromatography with photodiode array detector (HPLC-DAD) in systematic toxicological analysis. Forensic Sci Int 212:215–226CrossRefPubMed

15.

Pelander A, Tyrkkö E, Ojanperä I (2009) In silico methods for predicting metabolism and mass fragmentation applied to quetiapine in liquid chromatography/time-of-flight mass spectrometry urine drug screening. Rapid Commun Mass Spectrom 23:506–514CrossRefPubMed

16.

Davis PC, Bravo O, Gehrke M, Azumaya CT (2010) Development and validation of an LC-MS/MS method for the determination of quetiapine and four related metabolites in human plasma. J Pharm Biomed Anal 51:1113–1119CrossRefPubMed

17.

Fisher DS, Handley SA, Taylor D, Flanagan RJ (2012) Measurement of quetiapine and four quetiapine metabolites in human plasma by LC-MS/MS. Biomed Chromatogr 26:1125–1132CrossRefPubMed

Titel: Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry
verfasst von: Hideki Nozawa
Kayoko Minakata
Itaru Yamagishi
Koutaro Hasegawa
Masako Suzuki
Kunio Gonmori
Osamu Suzuki
Kanako Watanabe
Publikationsdatum: 01.04.2016
Verlag: Springer Japan
Erschienen in: Forensic Toxicology / Ausgabe 2/2016
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-016-0313-1

Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

Springer Medizin

Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2016

The 2-alkyl-2H-indazole regioisomers of synthetic cannabinoids AB-CHMINACA, AB-FUBINACA, AB-PINACA, and 5F-AB-PINACA are possible manufacturing impurities with cannabimimetic activities

Old opioids, new concerns: the case of acetylfentanyl

Successful quantification of 4′-methyl-α-pyrrolidinohexanophenone (MPHP) in human urine using LC–TOF-MS in an autopsy case

In vitro elucidation of the metabolic profile of the synthetic cannabinoid receptor agonists JWH-175 and JWH-176

Pharmacokinetics of heroin and its metabolites in vitreous humor and blood in a living pig model

Blood concentrations of α-pyrrolidinovalerophenone (α-PVP) determined in 66 forensic samples

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie