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European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

01.08.2008 | Article

siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells

verfasst von: C.-Y. Lu, H.-Y. Huang, T.-H. Yang, L.-Y. Chang, C.-Y. Lee, L.-M. Huang

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 8/2008

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Abstract

The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.

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Titel: siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
verfasst von: C.-Y. Lu
H.-Y. Huang
T.-H. Yang
L.-Y. Chang
C.-Y. Lee
L.-M. Huang
Publikationsdatum: 01.08.2008
Verlag: Springer-Verlag
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 8/2008
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-008-0495-5

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