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Journal of Bone and Mineral Metabolism

Erschienen in:

01.11.2010 | Original Article

Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells

verfasst von: Ryota Itoh, Shigenori Miura, Aki Takimoto, Shunya Kondo, Hiroko Sano, Yuji Hiraki

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 6/2010

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Abstract

Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are bioactive lysophospholipids that affect various cellular processes through G protein-coupled receptors. In our current study, we found by in situ hybridization that E11.5 mouse embryos strongly expressed the LPA receptor subtype LPA₁ in cartilaginous bone primordia and the surrounding mesenchymal cells. However, despite their wide-ranging actions, the roles of lysophospholipids in chondrogenesis remain poorly understood. The mouse clonal cell line ATDC5 undergoes a sequential differentiation of chondroprogenitor cells in vitro. Undifferentiated and differentiated ATDC5 cells express LPA₁ and other lysophospholipid receptors including S1P receptor S1P₁ and S1P₂. Taking advantage of this cell model, we studied the effects of LPA on the activities of chondroprogenitor cells. LPA markedly stimulates both DNA synthesis and the migration of ATDC5 chondroprogenitor cells in culture, whereas S1P suppresses the migration of these cells. Treatment with Ki16425, an LPA₁- and LPA₃-specific receptor antagonist, suppressed the fetal bovine serum-stimulated migration of ATDC5 cells by almost 80%. These results indicate that LPA plays an important role in the activation of chondroprogenitor cells.

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Titel: Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells
verfasst von: Ryota Itoh
Shigenori Miura
Aki Takimoto
Shunya Kondo
Hiroko Sano
Yuji Hiraki
Publikationsdatum: 01.11.2010
Verlag: Springer Japan
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 6/2010
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-010-0184-1

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