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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 8/2011

01.08.2011 | Genetics

Study of GT-repeat expansion in Heme oxygenase-1 gene promoter as genetic cause of male infertility

verfasst von: Elham Siasi, Ahmad Aleyasin, Seyed Javad Mowla, Hamid Sahebkashaf

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2011

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Abstract

Purpose

The length of GT-repeats polymorphic region in the promoter of human Heme oxygenase-1 gene (HO-1) alters the level of its transcriptional activity in response to oxidative stresses. Decreased level of HO-1 protein in the seminal plasma has been reported to be associated with oligospermia and azoospermia in male infertility. This is the first study to investigate the association between GT-repeats expansion in the promoter of the HO-1 gene and male infertility.

Methods

The frequencies of different GT-repeats alleles in the promoter of HO-1 gene were determined in 100 cases and 100 normal controls using PCR-PAGE, ABI fragment analysis genotyping and sequencing analysis.

Results

All alleles were classified into S and L alleles. S alleles were specified as number 0 to 3 with <27 GT-repeats and L alleles were specified as number 4 to 6 with >27 repeats. The L allele frequency was significantly higher among case group (54.5%) than that was obtained in the normal control group (37.5%). Statistical analysis provided a significant relationship between L allele and male infertility (P < 0.001).

Conclusions

This study shows for the first time that GT-repeats expansion in promoter of the HO-1 gene is associated with oligospermia and azoospermia among Iranian infertile cases.
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Metadaten
Titel
Study of GT-repeat expansion in Heme oxygenase-1 gene promoter as genetic cause of male infertility
verfasst von
Elham Siasi
Ahmad Aleyasin
Seyed Javad Mowla
Hamid Sahebkashaf
Publikationsdatum
01.08.2011
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 8/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-011-9574-0

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