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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 8/2011

01.08.2011 | Technical Innovations

Biological pH buffers in IVF: help or hindrance to success

verfasst von: Matthew A. Will, Natalie A. Clark, Jason E. Swain

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2011

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Abstract

Purpose

Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures

Methods

Keyword searches were performed using Pubmed and Medline and relevant literature reviewed.

Results

Various pH buffers have been used with varying degrees of success for gamete and embryo processing in a variety of animal species, as well as in human.

Conclusion

Though biologic buffers off a means to improve pH stability, not all buffers may be appropriate for use with gametes and embryos. Specific buffers may have undesired effects, and these may be buffer, species, cell type or concentration dependent. Continued research is needed to further refine and improve the use of biologic buffers for use in human ART.
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Metadaten
Titel
Biological pH buffers in IVF: help or hindrance to success
verfasst von
Matthew A. Will
Natalie A. Clark
Jason E. Swain
Publikationsdatum
01.08.2011
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 8/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-011-9582-0

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