nach oben

Erschienen in:

01.11.2009 | Overview

The possible use of stem cells in regenerative medicine: dream or reality?

verfasst von: Sabrina Ehnert, Matthias Glanemann, Andreas Schmitt, Stephan Vogt, Naama Shanny, Natascha C. Nussler, Ulrich Stöckle, Andreas Nussler

Erschienen in: Langenbeck's Archives of Surgery | Ausgabe 6/2009

Einloggen, um Zugang zu erhalten

Abstract

Stem cells are one of the most fascinating areas in regenerative medicine today. They play a crucial role in the development and regeneration of human life and are defined as cells that continuously reproduce themselves while maintaining the ability to differentiate into various cell types. Stem cells are found at all developmental stages, from embryonic stem cells that differentiate into all cell types found in the human body to adult stem cells that are responsible for tissue regeneration. The general opinion postulates that clinical therapies based on the properties of stem cells may have the potential to change the treatment of degenerative diseases or important traumatic injuries in the “near” future. We here briefly review the literature in particularly for the liver, heart, kidney, cartilage, and bone regeneration.

Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147PubMedCrossRef

Rubart M, Field LJ (2006) Cardiac regeneration: repopulating the heart. Annu Rev Physiol 68:29–49PubMedCrossRef

Chen N, Hudson JE, Walczak P et al (2005) Human umbilical cord blood progenitors: the potential of these hematopoietic cells to become neural. Stem Cells 23:1560–1570PubMedCrossRef

Ruhnke M, Nussler AK, Ungefroren H et al (2005) Human monocyte-derived neohepatocytes: a promising alternative to primary human hepatocytes for autologous cell therapy. Transplantation 79:1097–1103PubMedCrossRef

Ruhnke M, Ungefroren H, Nussler A et al (2005) Differentiation of in vitro-modified human peripheral blood monocytes into hepatocyte-like and pancreatic islet-like cells. Gastroenterology 128:1774–1786PubMedCrossRef

Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676PubMedCrossRef

Okita K, Ichisaka T, Yamanaka S (2007) Generation of germline-competent induced pluripotent stem cells. Nature 448:313–317PubMedCrossRef

Yu J, Vodyanik MA, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920PubMedCrossRef

Wernig M, Meissner A, Foreman R et al (2007) In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448:318–324PubMedCrossRef

10.

Lowry WE, Richter L, Yachechko R et al (2008) Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A 105:2883–2888PubMedCrossRef

11.

Park IH, Zhao R, West JA et al (2008) Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451:141–146PubMedCrossRef

12.

Kim JB, Zaehres H, Wu G et al (2008) Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature 454:646–650PubMedCrossRef

13.

Carey BW, Markoulaki S, Hanna J et al (2009) Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A 106:157–162PubMedCrossRef

14.

Li W, Wei W, Zhu S et al (2009) Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4:16–19PubMedCrossRef

15.

Levesque JP, Hendy J, Winkler IG et al (2003) Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cells. Exp Hematol 31:109–117PubMedCrossRef

16.

Petit I, Szyper-Kravitz M, Nagler A et al (2002) G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4. Nat Immunol 3:687–694PubMedCrossRef

17.

Francois S, Mouiseddine M, Mathieu N et al (2007) Human mesenchymal stem cells favour healing of the cutaneous radiation syndrome in a xenogenic transplant model. Ann Hematol 86:1–8PubMedCrossRef

18.

Gabelein G, Nussler AK, Morgott F et al (2008) Intrasplenic or subperitoneal hepatocyte transplantation to increase survival after surgically induced hepatic failure? Eur Surg Res 41:253–259PubMedCrossRef

19.

Harada K, Higaki S, Hashimoto K et al (2007) Study on the colonoscopic features of GVHD enteritis that developed after hematopoietic stem cell transplantation. Hepatogastroenterology 54:2221–2227PubMed

20.

Hauger O, Frost EE, van Heeswijk R et al (2006) MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy. Radiology 238:200–210PubMedCrossRef

21.

Inagaki Y, Higashiyama R, Okazaki I (2007) Treatment strategy for liver fibrosis through recruitment and differentiation of bone marrow stem/progenitor cells. Hepatol Res 37:991–993PubMedCrossRef

22.

Kawada H, Fujita J, Kinjo K et al (2004) Nonhematopoietic mesenchymal stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction. Blood 104:3581–3587PubMedCrossRef

23.

Oyagi S, Hirose M, Kojima M et al (2006) Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats. J Hepatol 44:742–748PubMedCrossRef

24.

Terai S, Yamamoto N, Omori K et al (2002) A new cell therapy using bone marrow cells to repair damaged liver. J Gastroenterol 37(Suppl 14):162–163PubMed

25.

Van Laake LW, Van Hoof D, Mummery CL (2005) Cardiomyocytes derived from stem cells. Ann Med 37:499–512PubMedCrossRef

26.

Glanemann M, Gaebelein G, Nussler N et al (2009) Transplantation of monocyte-derived hepatocyte-like cells (NeoHeps) improves survival in a model of acute liver failure. Ann Surg 249:149–154PubMedCrossRef

27.

Horwitz EM, Gordon PL, Koo WK et al (2002) Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Proc Natl Acad Sci U S A 99:8932–8937PubMedCrossRef

28.

Lazarus HM, Koc ON, Devine SM et al (2005) Cotransplantation of HLA-identical sibling culture-expanded mesenchymal stem cells and hematopoietic stem cells in hematologic malignancy patients. Biol Blood Marrow Transplant 11:389–398PubMedCrossRef

29.

Hidaka M, Su GN, Chen JK et al (2007) Transplantation of engineered bone tissue using a rotary three-dimensional culture system. In Vitro Cell Dev Biol Anim 43:49–58PubMedCrossRef

30.

Marra KG, Defail AJ, Clavijo-Alvarez JA et al (2008) FGF-2 enhances vascularization for adipose tissue engineering. Plast Reconstr Surg 121:1153–1164PubMedCrossRef

31.

He L, Nan X, Wang Y et al (2007) Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells. Sci China C Life Sci 50:429–437PubMedCrossRef

32.

Ma PX (2008) Biomimetic materials for tissue engineering. Adv Drug Deliv Rev 60:184–198PubMedCrossRef

33.

Bosetti M, Santin M, Lloyd AW et al (2007) Cell behaviour on phospholipids-coated surfaces. J Mater Sci Mater Med 18:611–617PubMedCrossRef

34.

Liu X, Won Y, Ma PX (2005) Surface modification of interconnected porous scaffolds. J Biomed Mater Res A 74:84–91PubMed

35.

Tachibana A, Nishikawa Y, Nishino M et al (2006) Modified keratin sponge: binding of bone morphogenetic protein-2 and osteoblast differentiation. J Biosci Bioeng 102:425–429PubMedCrossRef

36.

Ueblacker P, Wagner B, Vogt S et al (2007) In vivo analysis of retroviral gene transfer to chondrocytes within collagen scaffolds for the treatment of osteochondral defects. Biomaterials 28:4480–4487PubMedCrossRef

37.

Lucke M, Wildemann B, Sadoni S et al (2005) Systemic versus local application of gentamicin in prophylaxis of implant-related osteomyelitis in a rat model. Bone 36:770–778PubMedCrossRef

38.

Schmidmaier G, Wildemann B, Bail H et al (2001) Local application of growth factors (insulin-like growth factor-1 and transforming growth factor-beta1) from a biodegradable poly(D, L-lactide) coating of osteosynthetic implants accelerates fracture healing in rats. Bone 28:341–350PubMedCrossRef

39.

Ameen C, Strehl R, Bjorquist P et al (2008) Human embryonic stem cells: current technologies and emerging industrial applications. Crit Rev Oncol Hematol 65:54–80PubMedCrossRef

40.

Jensen J, Hyllner J, Bjorquist P (2009) Human embryonic stem cell technologies and drug discovery. J Cell Physiol 219:513–519PubMedCrossRef

41.

Molne J, Bjorquist P, Andersson K et al (2008) Blood group ABO antigen expression in human embryonic stem cells and in differentiated hepatocyte- and cardiomyocyte-like cells. Transplantation 86:1407–1413PubMedCrossRef

42.

Soderdahl T, Kuppers-Munther B, Heins N et al (2007) Glutathione transferases in hepatocyte-like cells derived from human embryonic stem cells. Toxicol In Vitro 21:929–937PubMedCrossRef

43.

Sartipy P, Bjorquist P, Strehl R et al (2007) The application of human embryonic stem cell technologies to drug discovery. Drug Discov Today 12:688–699PubMedCrossRef

44.

Ehnert S, Nussler AK, Lehmann A et al (2008) Blood monocyte-derived neohepatocytes as in vitro test system for drug metabolism. Drug Metab Dispos 36:1922–1929PubMedCrossRef

45.

Vigneau C, Zheng F, Polgar K et al (2006) Stem cells and kidney injury. Curr Opin Nephrol Hypertens 15:238–244PubMedCrossRef

46.

Duan Y, Catana A, Meng Y et al (2007) Differentiation and enrichment of hepatocyte-like cells from human embryonic stem cells in vitro and in vivo. Stem Cells 25:3058–3068PubMedCrossRef

47.

Isaikina Y, Kustanovich A, Svirnovski A (2006) Growth kinetics and self-renewal of human mesenchymal stem cells derived from bone marrow of children with oncohematological diseases during expansion in vitro. Exp Oncol 28:146–151PubMed

48.

Stagg J (2008) Mesenchymal stem cells in cancer. Stem Cell Rev 4:119–124PubMedCrossRef

49.

Strom S, Fisher R (2003) Hepatocyte transplantation: new possibilities for therapy. Gastroenterology 124:568–571PubMedCrossRef

50.

Ott M, Schmidt HH, Cichon G et al (2000) Emerging therapies in hepatology: liver-directed gene transfer and hepatocyte transplantation. Cells Tissues Organs 167:81–87PubMedCrossRef

51.

Sokal EM, Smets F, Bourgois A et al (2003) Hepatocyte transplantation in a 4-year-old girl with peroxisomal biogenesis disease: technique, safety, and metabolic follow-up. Transplantation 76:735–738PubMedCrossRef

52.

Kobayashi N, Miyazaki M, Fukaya K et al (2000) Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes. Cell Transplant 9:733–735PubMed

53.

Nagata H, Ito M, Shirota C et al (2003) Route of hepatocyte delivery affects hepatocyte engraftment in the spleen. Transplantation 76:732–734PubMedCrossRef

54.

Mito M, Kusano M, Kawaura Y (1992) Hepatocyte transplantation in man. Transplant Proc 24:3052–3053PubMed

55.

Strom SC, Chowdhury JR, Fox IJ (1999) Hepatocyte transplantation for the treatment of human disease. Semin Liver Dis 19:39–48PubMedCrossRef

56.

Aoki T, Koizumi T, Kobayashi Y et al (2005) A novel method of cryopreservation of rat and human hepatocytes by using encapsulation technique and possible use for cell transplantation. Cell Transplant 14:609–620PubMedCrossRef

57.

Ringel M, von Mach MA, Santos R et al (2005) Hepatocytes cultured in alginate microspheres: an optimized technique to study enzyme induction. Toxicology 206:153–167PubMedCrossRef

58.

Chan C, Berthiaume F, Nath BD et al (2004) Hepatic tissue engineering for adjunct and temporary liver support: critical technologies. Liver Transpl 10:1331–1342PubMedCrossRef

59.

Aurich H, Sgodda M, Kaltwasser P et al (2009) Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo. Gut 58:570–581PubMedCrossRef

60.

Aurich I, Mueller LP, Aurich H et al (2007) Functional integration of hepatocytes derived from human mesenchymal stem cells into mouse livers. Gut 56:405–415PubMedCrossRef

61.

Banas A, Teratani T, Yamamoto Y et al (2007) Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes. Hepatology 46:219–228PubMedCrossRef

62.

Ishii K, Yoshida Y, Akechi Y et al (2008) Hepatic differentiation of human bone marrow-derived mesenchymal stem cells by tetracycline-regulated hepatocyte nuclear factor 3beta. Hepatology 48:597–606PubMedCrossRef

63.

Jones EA, Tosh D, Wilson DI et al (2002) Hepatic differentiation of murine embryonic stem cells. Exp Cell Res 272:15–22PubMedCrossRef

64.

Lysy PA, Smets F, Sibille C et al (2007) Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation. Hepatology 46:1574–1585PubMedCrossRef

65.

Momose Y, Matsunaga T, Murai K et al (2009) Differentiation of monkey embryonic stem cells into hepatocytes and mRNA expression of cytochrome p450 enzymes responsible for drug metabolism: comparison of embryoid body formation conditions and matrices. Biol Pharm Bull 32:619–626PubMedCrossRef

66.

Najimi M, Khuu DN, Lysy PA et al (2007) Adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes? Cell Transplant 16:717–728PubMed

67.

Ruhnke M, Ungefroren H, Zehle G et al (2003) Long-term culture and differentiation of rat embryonic stem cell-like cells into neuronal, glial, endothelial, and hepatic lineages. Stem Cells 21:428–436PubMedCrossRef

68.

Shen CN, Slack JM, Tosh D (2000) Molecular basis of transdifferentiation of pancreas to liver. Nat Cell Biol 2:879–887PubMedCrossRef

69.

Kobayashi N, Westerman KA, Tanaka N et al (2001) A reversibly immortalized human hepatocyte cell line as a source of hepatocyte-based biological support. Addict Biol 6:293–300PubMedCrossRef

70.

Tosh D, Shen CN, Slack JM (2002) Differentiated properties of hepatocytes induced from pancreatic cells. Hepatology 36:534–543PubMedCrossRef

71.

Hengstler JG, Brulport M, Schormann W et al (2005) Generation of human hepatocytes by stem cell technology: definition of the hepatocyte. Expert Opin Drug Metab Toxicol 1:61–74PubMedCrossRef

72.

Nussler A, Konig S, Ott M et al (2006) Present status and perspectives of cell-based therapies for liver diseases. J Hepatol 45:144–159PubMedCrossRef

73.

Anversa P, Rota M, Urbanek K et al (2005) Myocardial aging–a stem cell problem. Basic Res Cardiol 100:482–493PubMedCrossRef

74.

Leri A, Kajstura J, Anversa P (2005) Cardiac stem cells and mechanisms of myocardial regeneration. Physiol Rev 85:1373–1416PubMedCrossRef

75.

Kamihata H, Matsubara H, Nishiue T et al (2001) Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines. Circulation 104:1046–1052PubMedCrossRef

76.

Orlic D, Kajstura J, Chimenti S et al (2001) Bone marrow cells regenerate infarcted myocardium. Nature 410:701–705PubMedCrossRef

77.

Erdo F, Buhrle C, Blunk J et al (2003) Host-dependent tumorigenesis of embryonic stem cell transplantation in experimental stroke. J Cereb Blood Flow Metab 23:780–785PubMedCrossRef

78.

Min JY, Yang Y, Converso KL et al (2002) Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats. J Appl Physiol 92:288–296PubMedCrossRef

79.

Angelini P, Markwald RR (2005) Stem cell treatment of the heart: a review of its current status on the brink of clinical experimentation. Tex Heart Inst J 32:479–488PubMed

80.

Segers VF, Lee RT (2008) Stem-cell therapy for cardiac disease. Nature 451:937–942PubMedCrossRef

81.

Yousef M, Schannwell CM, Kostering M et al (2009) The BALANCE Study: clinical benefit and long-term outcome after intracoronary autologous bone marrow cell transplantation in patients with acute myocardial infarction. J Am Coll Cardiol 53:2262–2269PubMedCrossRef

82.

Menasche P, Hagege AA, Vilquin JT et al (2003) Autologous skeletal myoblast transplantation for severe postinfarction left ventricular dysfunction. J Am Coll Cardiol 41:1078–1083PubMedCrossRef

83.

Hagege AA, Carrion C, Menasche P et al (2003) Viability and differentiation of autologous skeletal myoblast grafts in ischaemic cardiomyopathy. Lancet 361:491–492PubMedCrossRef

84.

Menasche P (2005) Stem cells for clinical use in cardiovascular medicine: current limitations and future perspectives. Thromb Haemost 94:697–701PubMed

85.

Menasche P, Alfieri O, Janssens S et al (2008) The Myoblast Autologous Grafting in Ischemic Cardiomyopathy (MAGIC) trial: first randomized placebo-controlled study of myoblast transplantation. Circulation 117:1189–1200PubMedCrossRef

86.

Tse HF, Kwong YL, Chan JK et al (2003) Angiogenesis in ischaemic myocardium by intramyocardial autologous bone marrow mononuclear cell implantation. Lancet 361:47–49PubMedCrossRef

87.

Ye L, Haider H, Sim EK (2006) Adult stem cells for cardiac repair: a choice between skeletal myoblasts and bone marrow stem cells. Exp Biol Med (Maywood) 231:8–19

88.

Cleland JG, Coletta AP, Abdellah AT et al (2007) Clinical trials update from the American Heart Association 2006: OAT, SALT 1 and 2, MAGIC, ABCD, PABA-CHF, IMPROVE-CHF, and percutaneous mitral annuloplasty. Eur J Heart Fail 9:92–97PubMedCrossRef

89.

Murry CE, Soonpaa MH, Reinecke H et al (2004) Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428:664–668PubMedCrossRef

90.

Stamm C, Westphal B, Kleine HD et al (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 361:45–46PubMedCrossRef

91.

Laham RJ, Oettgen P (2003) Bone marrow transplantation for the heart: fact or fiction? Lancet 361:11–12PubMedCrossRef

92.

Brodie JC, Humes HD (2005) Stem cell approaches for the treatment of renal failure. Pharmacol Rev 57:299–313PubMedCrossRef

93.

Lin F (2006) Stem cells in kidney regeneration following acute renal injury. Pediatr Res 59:74R–78RPubMedCrossRef

94.

Ricardo SD, Deane JA (2005) Adult stem cells in renal injury and repair. Nephrology (Carlton) 10:276–282CrossRef

95.

Oliver JA, Maarouf O, Cheema FH et al (2004) The renal papilla is a niche for adult kidney stem cells. J Clin Invest 114:795–804PubMed

96.

Morigi M, Imberti B, Zoja C et al (2004) Mesenchymal stem cells are renotropic, helping to repair the kidney and improve function in acute renal failure. J Am Soc Nephrol 15:1794–1804PubMedCrossRef

97.

Kale S, Karihaloo A, Clark PR et al (2003) Bone marrow stem cells contribute to repair of the ischemically injured renal tubule. J Clin Invest 112:42–49PubMed

98.

Anglani F, Forino M, Del Prete D et al (2004) In search of adult renal stem cells. J Cell Mol Med 8:474–487PubMedCrossRef

99.

Bates CM, Lin F (2005) Future strategies in the treatment of acute renal failure: growth factors, stem cells, and other novel therapies. Curr Opin Pediatr 17:215–220PubMedCrossRef

100.

Hangody L, Vasarhelyi G, Hangody LR et al (2008) Autologous osteochondral grafting–technique and long-term results. Injury 39(Suppl 1):S32–S39PubMedCrossRef

101.

Steinwachs MR, Guggi T, Kreuz PC (2008) Marrow stimulation techniques. Injury 39(Suppl 1):S26–S31PubMedCrossRef

102.

Salama R, Weissman SL (1978) The clinical use of combined xenografts of bone and autologous red marrow. A preliminary report. J Bone Joint Surg Br 60:111–115PubMed

103.

Goel A, Sangwan SS, Siwach RC et al (2005) Percutaneous bone marrow grafting for the treatment of tibial non-union. Injury 36:203–206PubMedCrossRef

104.

Marcacci M, Kon E, Moukhachev V et al (2007) Stem cells associated with macroporous bioceramics for long bone repair: 6- to 7-year outcome of a pilot clinical study. Tissue Eng 13:947–955PubMedCrossRef

105.

Raty JK, Lesch HP, Wirth T et al (2008) Improving safety of gene therapy. Curr Drug Saf 3:46–53PubMedCrossRef

106.

Yi Y, Hahm SH, Lee KH (2005) Retroviral gene therapy: safety issues and possible solutions. Curr Gene Ther 5:25–35PubMed

107.

Strom SC, Fisher RA, Thompson MT et al (1997) Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure. Transplantation 63:559–569PubMedCrossRef

108.

Fox IJ, Chowdhury JR, Kaufman SS et al (1998) Treatment of the Crigler-Najjar syndrome type I with hepatocyte transplantation. N Engl J Med 338:1422–1426PubMedCrossRef

109.

Bohnen NI, Charron M, Reyes J et al (2000) Use of indium-111-labeled hepatocytes to determine the biodistribution of transplanted hepatocytes through portal vein infusion. Clin Nucl Med 25:447–450PubMedCrossRef

110.

Horslen SP, McCowan TC, Goertzen TC et al (2003) Isolated hepatocyte transplantation in an infant with a severe urea cycle disorder. Pediatrics 111:1262–1267PubMedCrossRef

111.

Muraca M, Gerunda G, Neri D et al (2002) Hepatocyte transplantation as a treatment for glycogen storage disease type 1a. Lancet 359:317–318PubMedCrossRef

112.

Dhawan A, Mitry RR, Hughes RD et al (2004) Hepatocyte transplantation for inherited factor VII deficiency. Transplantation 78:1812–1814PubMedCrossRef

113.

Meyburg J, Hoerster F, Weitz J et al (2008) Use of the middle colic vein for liver cell transplantation in infants and small children. Transplant Proc 40:936–937PubMedCrossRef

114.

Allen KJ, Mifsud NA, Williamson R et al (2008) Cell-mediated rejection results in allograft loss after liver cell transplantation. Liver Transpl 14:688–694PubMedCrossRef

115.

Ambrosino G, Varotto S, Strom SC et al (2005) Isolated hepatocyte transplantation for Crigler-Najjar syndrome type 1. Cell Transplant 14:151–157PubMedCrossRef

116.

Meyburg J, Das AM, Hoerster F et al (2009) One liver for four children: first clinical series of liver cell transplantation for severe neonatal urea cycle defects. Transplantation 87:636–641PubMedCrossRef

117.

Garg NK, Gaur S, Sharma S (1993) Percutaneous autogenous bone marrow grafting in 20 cases of ununited fracture. Acta Orthop Scand 64:671–672PubMedCrossRef

118.

Gan Y, Dai K, Zhang P et al (2008) The clinical use of enriched bone marrow stem cells combined with porous beta-tricalcium phosphate in posterior spinal fusion. Biomaterials 29:3973–3982PubMedCrossRef

Titel: The possible use of stem cells in regenerative medicine: dream or reality?
verfasst von: Sabrina Ehnert
Matthias Glanemann
Andreas Schmitt
Stephan Vogt
Naama Shanny
Natascha C. Nussler
Ulrich Stöckle
Andreas Nussler
Publikationsdatum: 01.11.2009
Verlag: Springer-Verlag
Erschienen in: Langenbeck's Archives of Surgery / Ausgabe 6/2009
Print ISSN: 1435-2443
Elektronische ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-009-0546-0

Neu im Fachgebiet Chirurgie

Vorsicht, erhöhte Blutungsgefahr nach PCI!

10.05.2024 Koronare Herzerkrankung Nachrichten

Nach PCI besteht ein erhöhtes Blutungsrisiko, wenn die Behandelten eine verminderte linksventrikuläre Ejektionsfraktion aufweisen. Das Risiko ist umso höher, je stärker die Pumpfunktion eingeschränkt ist.

Darf man die Behandlung eines Neonazis ablehnen?

08.05.2024 Gesellschaft Nachrichten

In einer Leseranfrage in der Zeitschrift Journal of the American Academy of Dermatology möchte ein anonymer Dermatologe bzw. eine anonyme Dermatologin wissen, ob er oder sie einen Patienten behandeln muss, der eine rassistische Tätowierung trägt.

Deutlich weniger Infektionen: Wundprotektoren schützen!

08.05.2024 Postoperative Wundinfektion Nachrichten

Der Einsatz von Wundprotektoren bei offenen Eingriffen am unteren Gastrointestinaltrakt schützt vor Infektionen im Op.-Gebiet – und dient darüber hinaus der besseren Sicht. Das bestätigt mit großer Robustheit eine randomisierte Studie im Fachblatt JAMA Surgery.

Chirurginnen und Chirurgen sind stark suizidgefährdet

07.05.2024 Suizid Nachrichten

Der belastende Arbeitsalltag wirkt sich negativ auf die psychische Gesundheit der Angehörigen ärztlicher Berufsgruppen aus. Chirurginnen und Chirurgen bilden da keine Ausnahme, im Gegenteil.

Update Chirurgie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Aktuelle BDC Leitlinien-Webinare

S3-Leitlinie „Diagnostik und Therapie des Karpaltunnelsyndroms“

Karpaltunnelsyndrom BDC Leitlinien Webinare

CME: 2 Punkte

Das Karpaltunnelsyndrom ist die häufigste Kompressionsneuropathie peripherer Nerven. Obwohl die Anamnese mit dem nächtlichen Einschlafen der Hand (Brachialgia parästhetica nocturna) sehr typisch ist, ist eine klinisch-neurologische Untersuchung und Elektroneurografie in manchen Fällen auch eine Neurosonografie erforderlich. Im Anfangsstadium sind konservative Maßnahmen (Handgelenksschiene, Ergotherapie) empfehlenswert. Bei nicht Ansprechen der konservativen Therapie oder Auftreten von neurologischen Ausfällen ist eine Dekompression des N. medianus am Karpaltunnel indiziert.

Prof. Dr. med. Gregor Antoniadis

S2e-Leitlinie „Distale Radiusfraktur“

Radiusfraktur BDC Leitlinien Webinare

CME: 2 Punkte

Das Webinar beschäftigt sich mit Fragen und Antworten zu Diagnostik und Klassifikation sowie Möglichkeiten des Ausschlusses von Zusatzverletzungen. Die Referenten erläutern, welche Frakturen konservativ behandelt werden können und wie. Das Webinar beantwortet die Frage nach aktuellen operativen Therapiekonzepten: Welcher Zugang, welches Osteosynthesematerial? Auf was muss bei der Nachbehandlung der distalen Radiusfraktur geachtet werden?

PD Dr. med. Oliver Pieske

Dr. med. Benjamin Meyknecht

S1-Leitlinie „Empfehlungen zur Therapie der akuten Appendizitis bei Erwachsenen“

Appendizitis BDC Leitlinien Webinare

CME: 2 Punkte

Inhalte des Webinars zur S1-Leitlinie „Empfehlungen zur Therapie der akuten Appendizitis bei Erwachsenen“ sind die Darstellung des Projektes und des Erstellungswegs zur S1-Leitlinie, die Erläuterung der klinischen Relevanz der Klassifikation EAES 2015, die wissenschaftliche Begründung der wichtigsten Empfehlungen und die Darstellung stadiengerechter Therapieoptionen.

Dr. med. Mihailo Andric

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2009

Visceral artery aneurysms—follow-up of 23 patients with 31 aneurysms after surgical or interventional therapy

Management of biliary complications following living donor liver transplantation—a single center experience

Expression of receptors tyrosine kinase c-kit and EGF-R in colorectal adenocarcinomas: is there a relationship with epithelial–mesenchymal transition during tumor progression?

Appendicitis score for children with suspected appendicitis. A randomized clinical trial

Surgery and adjuvant therapies in the treatment of stage IV melanoma: our experience in 84 patients

Caudate lobe resection: an Egyptian center experience

Update Chirurgie