nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.01.2009 | ANDROLOGY

The presence of bacteria species in semen and sperm quality

verfasst von: Elena Moretti, Serena Capitani, Natale Figura, Andrea Pammolli, Maria Grazia Federico, Valentina Giannerini, Giulia Collodel

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 1/2009

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To verify the prevalence of semen bacterial contamination and whether the contamination could decrease sperm quality.

Methods

Spermiogram, semen culture, and sperm transmission electron microscopy (TEM) analysis were performed. TEM data were elaborated using a mathematical formula that calculates a fertility index (FI)—able to define patients as fertile or infertile—and the percentage of sperm apoptosis, immaturity and necrosis. We aligned the amino acid sequence of beta-tubulin with protein of the most frequent species isolated from semen.

Results

Patients were divided according to the contaminating species; in each group, we observed fertile individuals, in whom the semen quality was similar to that of controls and infertile men whose sperm quality was significantly decreased, in terms of motility, FI, apoptosis and necrosis. Partial homology between β-tubulin and bacterial proteins was observed.

Conclusion

Sperm bacterial contamination is quite frequent and could contribute to the deterioration of the sperm quality of infertile men.

Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol 2008;140:3–11. doi:10.1016/j.ejogrb.2008.03.009.PubMedCrossRef

Henkel R, Schill WB. Sperm separation in patients with urogenital infections. Andrologia 1998;30:91–7.PubMed

Urata K, Narahara H, Tanaka Y, Egashira T, Takayama F, Miyakawa I. Effect of endotoxin-induced reactive oxygen species on sperm motility. Fertil Steril 2001;76:163–6. doi:10.1016/S0015-0282(01)01850-7.PubMedCrossRef

Sanocka-Maciejewska D, Ciupińska M, Kurpisz M. Bacterial infection and semen quality. J Reprod Immunol 2005;67:51–6. doi:10.1016/j.jri.2005.06.003.PubMedCrossRef

Purvis K, Christiansen E. Infection in the male reproductive tract. Impact, diagnosis and treatment in relation to male infertility. Int J Androl 1993;16:1–13. doi:10.1111/j.1365-2605.1993.tb01146.x.PubMedCrossRef

Diemer T, Huwe P, Ludwig M, Schroeder-Printzen I, Michelmann HW, Schiefer HG, et al. Influence of autogenous leucocytes and Escherichia coli on sperm motility parameters in vitro. Andrologia 2003;35:100–5. doi:10.1046/j.1439-0272.2003.00523.x.PubMedCrossRef

Diemer T, Huwe P, Michelmann HW, Mayer F, Schiefer HG, Weidner W. Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis. Int J Androl 2000;23:178–86. doi:10.1046/j.1365-2605.2000.00224.x.PubMedCrossRef

Mehta RH, Sridhar H, Vijay Kumar BR, Anand Kumar TC. High incidence of oligozoospermia and teratozoospermia in human semen infected with the aerobic bacterium Streptococcus faecalis. RBM Online 2002;5:17–21.PubMed

Qiang H, Jiang MS, Lin JY, He WM. Influence of enterococci on human sperm membrane in vitro. Asian J Androl 2007;9:77–81. doi:10.1111/j.1745-7262.2007.00219.x.PubMedCrossRef

10.

Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T, et al. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis 2007;7:129. doi:10.1186/1471-2334-7-129.PubMedCrossRef

11.

Fraczek M, Kurpisz M. Inflammatory mediators exert toxic effects of oxidative stress on human spermatozoa. J Androl 2007;28:325–33. doi:10.2164/jandrol.106.001149.PubMedCrossRef

12.

Ombelet W, Bosmans E, Janssen M, Cox A, Vlasselaer J, Gyselaers W, et al. Semen parameters in a fertile versus subfertile population: a need for change in the interpretation of semen testing. Hum Reprod 1997;12:987–93. doi:10.1093/humrep/12.5.987.PubMedCrossRef

13.

World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interactions. 4thth ed. Cambridge, United Kingdom: Cambridge University Press; 1999.

14.

Baccetti B, Bernieri G, Burrini AG, Collodel G, Crisà N, Mirolli M, et al. Notulae seminologicae. 5. Mathematical evaluation of interdependent submicroscopic sperm alterations. J Androl. 1995;16:356–71.PubMed

15.

Collodel G, Moretti E. Morphology and meiotic segregation in spermatozoa from men of proven fertility. J Androl 2008;29:106–14. doi:10.2164/jandrol.107.002998.PubMedCrossRef

16.

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–402. doi:10.1093/nar/25.17.3389.PubMedCrossRef

17.

Haidl G. Macrophages in semen are indicative of chronic epididymal infection. Arch Androl 1990;25:5–11. doi:10.3109/01485019008987587.PubMedCrossRef

18.

Tremellen K. Oxidative stress and male infertility—a clinical perspective. Hum Reprod Update 2008;14:243–58. doi:10.1093/humupd/dmn004.PubMedCrossRef

19.

Fraczek M, Szumala-Kakol A, Jedrzejczak P, Kamieniczna M, Kurpisz M. Bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation. Fertil Steril 2007;88:1076–85. doi:10.1016/j.fertnstert.2006.12.025.PubMedCrossRef

20.

Potts JM, Sharma R, Pasqualotto F, Nelson D, Hall G, Agarwal A. Association of ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. J Urol 2000;163:1775–8. doi:10.1016/S0022-5347(05)67540-4.PubMedCrossRef

21.

Villegas J, Schulz M, Soto L, Sanchez R. Bacteria induce expression of apoptosis in human spermatozoa. Apoptosis 2005;10:105–10. doi:10.1007/s10495-005-6065-8.PubMedCrossRef

22.

Fanibunda SE, Velhal SM, Raghavan VP, Bandivdekar AH. CD4 independent binding of HIV gp120 to mannose receptor on human spermatozoa. J Acquir Immune Defic Syndr 2008;48:389–97.PubMedCrossRef

23.

Fujita K, Yokota T, Oguri T, Fujime M, Kitagawa R. In vitro adherence of Staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus aureus to human ureter. Urol Res 1992;6:399–402. doi:10.1007/BF00294495.CrossRef

24.

Nallapareddy SR, Singh KV, Sillanpää J, Garsin DA, Höök M, Erlandsen SL, et al. Endocarditis and biofilm-associated pili of Enterococcus faecalis. J Clin Invest 2006;116:2582–4. doi:10.1172/JCI29021.CrossRef

25.

Baccetti BM, Bruni E, Capitani S, Collodel G, Mancini S, Piomboni P, et al. Studies on varicocele III: ultrastructural sperm evaluation and 18, X and Y aneuploidies. J Androl 2006;27:94–101. doi:10.2164/jandrol.05081.PubMedCrossRef

26.

Collodel G, Baccetti B, Capitani S, Moretti E. Necrosis in human spermatozoa. I. Ultrastructural features and FISH study in semen from patients with uro-genital infections. J Submicrosc Cytol Pathol 2005;37:67–73.PubMed

27.

Grassmé H, Jendrossek V, Gulbins E. Molecular mechanisms of bacteria induced apoptosis. Apoptosis 2001;6:441–5. doi:10.1023/A:1012485506972.PubMedCrossRef

28.

Eggert-Kruse W, Rohr G, Ströck W, Pohl S, Schwalbach B, Runnebaum B. Anaerobes in ejaculates of subfertile men. Hum Reprod Update 1995;1:462–78. doi:10.1093/humupd/1.5.462.PubMedCrossRef

29.

Kiessling AA, Desmarais BM, Yin HZ, Loverde J, Eyre RC. Detection and identification of bacterial DNA in semen. Fertil Steril 2008;90:1744–56. doi:10.1016/j.fertnstert.2007.08.083.PubMedCrossRef

Titel: The presence of bacteria species in semen and sperm quality
verfasst von: Elena Moretti
Serena Capitani
Natale Figura
Andrea Pammolli
Maria Grazia Federico
Valentina Giannerini
Giulia Collodel
Publikationsdatum: 01.01.2009
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 1/2009
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9283-5

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

The presence of bacteria species in semen and sperm quality