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01.08.2009 | Original Article

The role of basic fibroblast growth factor to enhance fetal intestinal mucosal cell regeneration in vivo

verfasst von: Atsushi Yoshida, Takuo Noda, Morimichi Tani, Takanori Oyama, Yasuhiro Watanabe, Hideyasu Kiyomoto, Akira Nishiyama

Erschienen in: Pediatric Surgery International | Ausgabe 8/2009

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Abstract

Background/Purpose

The limited application of small bowel transplantation for short bowel syndrome, mainly on the account of the morbidity and long-term implications of the procedure, has led to a search for alternative therapies. The purpose of this study was to evaluate whether basic fibroblast growth factor (bFGF) could facilitate regeneration of fetal small intestinal mucosa in vivo.

Methods

Intestinal epithelial organoid units harvested from fetal Lewis rats were injected into adult male Lewis rats whose colon was denuded of mucosa, as syngeneic recipients. One experimental group transplanted with the addition of 50 ng/ml bFGF, was compared with a control group that were transplanted without bFGF. The grafts were harvested and analyzed using histology and immunohistochemistry 3 weeks after operation.

Results

There were 4 anesthetic deaths, two in each group, and 11 deaths due to adhesive ileus. In no rat did neomucosa fully cover the denuded colonic muscle throughout the whole length of lumen. Histologically, the structure of the neomucosa, when present, was normal small intestinal mucosa. The small intestinal mucosa was partially restored in 100% (6 of 6) of bFGF, and in 28.6% (2 of 7) of those not given bFGF (P = 0.0021).

Conclusions

These data demonstrate that bFGF can facilitate the restoration of intestinal epithelial cells, at least to some degree. Potentially, refinements of this technique could be used to facilitate the physiologic tissue engineering of small intestine in a way that allows it to move peristaltically, and have an application in the management of patients with short bowel syndrome.

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Titel: The role of basic fibroblast growth factor to enhance fetal intestinal mucosal cell regeneration in vivo
verfasst von: Atsushi Yoshida
Takuo Noda
Morimichi Tani
Takanori Oyama
Yasuhiro Watanabe
Hideyasu Kiyomoto
Akira Nishiyama
Publikationsdatum: 01.08.2009
Verlag: Springer-Verlag
Erschienen in: Pediatric Surgery International / Ausgabe 8/2009
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-009-2405-z

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The role of basic fibroblast growth factor to enhance fetal intestinal mucosal cell regeneration in vivo