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01.10.2014 | Original Article

Time-lapse observations to analyze the effects of assisted hatching

verfasst von: Maki Goto, Akira Iwase, Naomi Furusawa, Harumi Kobayashi, Nao Kato, Ai Saito, Masahiko Mori, Satoko Osuka, Mika Kondo, Tomoko Nakamura, Tatsuo Nakahara, Hiroyuki Matsumoto, Fumitaka Kikkawa

Erschienen in: Reproductive Medicine and Biology | Ausgabe 4/2014

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Abstract

Purpose

Assisted hatching (AH) is an artificial disruption of the zona pellucida with the aim of facilitating embryo implantation. We used time-lapse observations of mouse embryos to examine the effect of AH in mouse blastocysts.

Methods

AH techniques were performed with acid Tyrode’s solution. We compared the rates of blastocyst formation and blastocyst attachment to Ishikawa cells between the control (n = 28) and the AH group (n = 24). To analyze the effects of AH, 8-cell mice embryos were cultured under time-lapse observations (every 15 min). The time required for hatching, the hatching rates, the frequency of contraction, and the contraction rates in the blastocysts were analyzed.

Results

There were no significant differences between the two groups in hatching rate or attachment rate. The times required for hatching were 286 ± 22 min in the AH group and 990 ± 437 min in the control group (P = 0.018). The contraction frequencies in blastocysts were 3.5 ± 0.7 times in the AH group and 7.5 ± 2.5 times in the control group (P = 0.020).

Conclusions

From the time-lapse observations we found that the time required for hatching and the frequency of contraction in blastocysts were both reduced by AH, although blastocyst formation and attachment were not affected.

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Titel: Time-lapse observations to analyze the effects of assisted hatching
verfasst von: Maki Goto
Akira Iwase
Naomi Furusawa
Harumi Kobayashi
Nao Kato
Ai Saito
Masahiko Mori
Satoko Osuka
Mika Kondo
Tomoko Nakamura
Tatsuo Nakahara
Hiroyuki Matsumoto
Fumitaka Kikkawa
Publikationsdatum: 01.10.2014
Verlag: Springer Japan
Erschienen in: Reproductive Medicine and Biology / Ausgabe 4/2014
Print ISSN: 1445-5781
Elektronische ISSN: 1447-0578
DOI: https://doi.org/10.1007/s12522-014-0182-4

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Time-lapse observations to analyze the effects of assisted hatching