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Journal of Assisted Reproduction and Genetics

Erschienen in:

01.03.2016 | Assisted Reproduction Technologies

Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

verfasst von: Daniel Bodri, Satoshi Kawachiya, Takeshi Sugimoto, Jazmina Yao Serna, Ryutaro Kato, Tsunekazu Matsumoto

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2016

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Abstract

Purpose

The purpose of this study was to determine which morphokinetic variables are related to embryo gender in a cohort of consecutive live births obtained through single blastocyst transfer following mild ovarian stimulation.

Methods

Eighty-one live births (49 % of them females) from successfully treated, consecutive infertile patients (maternal age 36.9 ± 3.8 years, range 28–46) who underwent minimal ovarian stimulation, prolonged embryo culture in a time-lapse monitoring (TLM) incubator and elective single blastocyst transfers during 2012–2014. Early (PNf, t2–t9, cc2a, b, s2, s3) and late (tM, tSB, tfullB, texpB₁, and texpB₂) morphokinetic variables were scored according to published consensus criteria and were normalized to the time of pronuclear fading. For each variable, the ranges with the highest proportion of female embryos (optimal range) were determined by detailed examination of histograms.

Results

Female embryo gender was associated both with late cleavage (t8), morula (tM), and blastocyst stage morphokinetic variables. The strongest associations (adjusted ORs, 7.0–7.8) were found for late, expanded stage blastocyst parameters; tfullB, texpB₁, and texpB₂. The proportion of female embryos was 69–71 and 25–26 % inside and outside of the optimal ranges, respectively. This allowed to predict 74–78 % of them, increasing their proportion by 57 % compared to the average.

Conclusions

Although the sample size of our cohort was limited, our findings suggest that several expanded blastocyst stage morphokinetic parameters are associated with female embryo gender. If confirmed on a larger sample these could be potentially used to increase the proportion of female embryos among non-invasively selected blastocysts following single embryo transfer.

Kaser DJ, Racowsky C. Clinical outcomes following selection of human preimplantation embryos with time-lapse monitoring: a systematic review. Hum Reprod Update. 2014;20(5):617–31. doi:10.1093/humupd/dmu023.CrossRefPubMed

Campbell A, Fishel S, Bowman N, Duffy S, Sedler M, Hickman CF. Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod Biomed Online. 2013;26(5):477–85. doi:10.1016/j.rbmo.2013.02.006.CrossRefPubMed

Meseguer M, Herrero J, Tejera A, Hilligsoe KM, Ramsing NB, Remohi J. The use of morphokinetics as a predictor of embryo implantation. Hum Reprod. 2011;26(10):2658–71. doi:10.1093/humrep/der256.CrossRefPubMed

Dal Canto M, Coticchio G, Mignini Renzini M, De Ponti E, Novara PV, Brambillasca F, et al. Cleavage kinetics analysis of human embryos predicts development to blastocyst and implantation. Reprod Biomed Online. 2012;25(5):474–80. doi:10.1016/j.rbmo.2012.07.016.CrossRefPubMed

Rubio I, Galan A, Larreategui Z, Ayerdi F, Bellver J, Herrero J, et al. Clinical validation of embryo culture and selection by morphokinetic analysis: a randomized, controlled trial of the EmbryoScope. Fertil Steril. 2014;102(5):1287–94 e5. doi:10.1016/j.fertnstert.2014.07.738.CrossRefPubMed

VerMilyea MD, Tan L, Anthony JT, Conaghan J, Ivani K, Gvakharia M, et al. Computer-automated time-lapse analysis results correlate with embryo implantation and clinical pregnancy: a blinded, multi-centre study. Reprod Biomed Online. 2014;29(6):729–36. doi:10.1016/j.rbmo.2014.09.005.CrossRefPubMedPubMedCentral

Ciray HN, Aksoy T, Goktas C, Ozturk B, Bahceci M. Time-lapse evaluation of human embryo development in single versus sequential culture media--a sibling oocyte study. J Assist Reprod Genet. 2012;29(9):891–900. doi:10.1007/s10815-012-9818-7.CrossRefPubMedPubMedCentral

Cruz M, Garrido N, Gadea B, Munoz M, Perez-Cano I, Meseguer M. Oocyte insemination techniques are related to alterations of embryo developmental timing in an oocyte donation model. Reprod Biomed Online. 2013;27(4):367–75. doi:10.1016/j.rbmo.2013.06.017.CrossRefPubMed

Munoz M, Cruz M, Humaidan P, Garrido N, Perez-Cano I, Meseguer M. Dose of recombinant FSH and oestradiol concentration on day of HCG affect embryo development kinetics. Reprod Biomed Online. 2012;25(4):382–9. doi:10.1016/j.rbmo.2012.06.016.CrossRefPubMed

10.

Munoz M, Cruz M, Humaidan P, Garrido N, Perez-Cano I, Meseguer M. The type of GnRH analogue used during controlled ovarian stimulation influences early embryo developmental kinetics: a time-lapse study. Eur J Obstet Gynecol Reprod Biol. 2013;168(2):167–72. doi:10.1016/j.ejogrb.2012.12.038.CrossRefPubMed

11.

Wissing ML, Bjerge MR, Olesen AI, Hoest T, Mikkelsen AL. Impact of PCOS on early embryo cleavage kinetics. Reprod Biomed Online. 2014;28(4):508–14. doi:10.1016/j.rbmo.2013.11.017.CrossRefPubMed

12.

Kirkegaard K, Hindkjaer JJ, Ingerslev HJ. Effect of oxygen concentration on human embryo development evaluated by time-lapse monitoring. Fertil Steril. 2013;99(3):738–44 e4. doi:10.1016/j.fertnstert.2012.11.028.CrossRefPubMed

13.

Freour T, Dessolle L, Lammers J, Lattes S, Barriere P. Comparison of embryo morphokinetics after in vitro fertilization-intracytoplasmic sperm injection in smoking and nonsmoking women. Fertil Steril. 2013;99(7):1944–50. doi:10.1016/j.fertnstert.2013.01.136.CrossRefPubMed

14.

Serdarogullari M, Findikli N, Goktas C, Sahin O, Ulug U, Yagmur E, et al. Comparison of gender-specific human embryo development characteristics by time-lapse technology. Reprod Biomed Online. 2014;29(2):193–9. doi:10.1016/j.rbmo.2014.03.026.CrossRefPubMed

15.

Bronet F, Nogales MC, Martinez E, Ariza M, Rubio C, Garcia-Velasco JA, et al. Is there a relationship between time-lapse parameters and embryo sex? Fertil Steril. 2015;103(2):396–401 e2. doi:10.1016/j.fertnstert.2014.10.050.CrossRefPubMed

16.

Weston G, Osianlis T, Catt J, Vollenhoven B. Blastocyst transfer does not cause a sex-ratio imbalance. Fertil Steril. 2009;92(4):1302–5. doi:10.1016/j.fertnstert.2008.07.1784.CrossRefPubMed

17.

Alfarawati S, Fragouli E, Colls P, Stevens J, Gutierrez-Mateo C, Schoolcraft WB, et al. The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender. Fertil Steril. 2011;95(2):520–4. doi:10.1016/j.fertnstert.2010.04.003.CrossRefPubMed

18.

Richter KS, Anderson M, Osborn BH. Selection for faster development does not bias sex ratios resulting from blastocyst embryo transfer. Reprod Biomed Online. 2006;12(4):460–5.CrossRefPubMed

19.

Hentemann MA, Briskemyr S, Bertheussen K. Blastocyst transfer and gender: IVF versus ICSI. J Assist Reprod Genet. 2009;26(8):433–6. doi:10.1007/s10815-009-9337-3.CrossRefPubMedPubMedCentral

20.

Kato K, Takehara Y, Segawa T, Kawachiya S, Okuno T, Kobayashi T, et al. Minimal ovarian stimulation combined with elective single embryo transfer policy: age-specific results of a large, single-centre, Japanese cohort. Reprod Biol Endocrinol. 2012;10:35. doi:10.1186/1477-7827-10-35.CrossRefPubMedPubMedCentral

21.

Bodri D, Kawachiya S, Kondo M, Kato R, Matsumoto T. Oocyte retrieval timing based on spontaneous luteinizing hormone surge during natural cycle in vitro fertilization treatment. Fertil Steril. 2014;101(4):1001–7 e2. doi:10.1016/j.fertnstert.2014.01.016.CrossRefPubMed

22.

Alpha Scientists in Reproductive M, Embryology ESIGo. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26(6):1270–83. doi:10.1093/humrep/der037.CrossRef

23.

Kato K, Ueno S, Yabuuchi A, Uchiyama K, Okuno T, Kobayashi T, et al. Women’s age and embryo developmental speed accurately predict clinical pregnancy after single vitrified-warmed blastocyst transfer. Reprod Biomed Online. 2014;29(4):411–6. doi:10.1016/j.rbmo.2014.06.007.CrossRefPubMed

24.

Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2007;67(1):73–80. doi:10.1016/j.theriogenology.2006.09.014.CrossRefPubMed

25.

Kirkegaard K, Kesmodel US, Hindkjaer JJ, Ingerslev HJ. Time-lapse parameters as predictors of blastocyst development and pregnancy outcome in embryos from good prognosis patients: a prospective cohort study. Hum Reprod. 2013;28(10):2643–51. doi:10.1093/humrep/det300.CrossRefPubMed

26.

Gardner DK, Surrey E, Minjarez D, Leitz A, Stevens J, Schoolcraft WB. Single blastocyst transfer: a prospective randomized trial. Fertil Steril. 2004;81(3):551–5. doi:10.1016/j.fertnstert.2003.07.023.CrossRefPubMed

27.

Zhang J, Chang L, Sone Y, Silber S. Minimal ovarian stimulation (mini-IVF) for IVF utilizing vitrification and cryopreserved embryo transfer. Reprod Biomed Online. 2010;21(4):485–95. doi:10.1016/j.rbmo.2010.06.033.CrossRefPubMed

28.

Ciray HN, Campbell A, Agerholm IE, Aguilar J, Chamayou S, Esbert M, et al. Proposed guidelines on the nomenclature and annotation of dynamic human embryo monitoring by a time-lapse user group. Hum Reprod. 2014;29(12):2650–60. doi:10.1093/humrep/deu278.CrossRefPubMed

29.

Bodri D, Sugimoto T, Serna JY, Kondo M, Kato R, Kawachiya S, et al. Influence of different oocyte insemination techniques on early and late morphokinetic parameters: retrospective analysis of 500 time-lapse monitored blastocysts. Fertil Steril. 2015;104(5):1175–81 e2. doi:10.1016/j.fertnstert.2015.07.1164.CrossRefPubMed

30.

Liu Y, Chapple V, Feenan K, Roberts P, Matson P. Time-lapse videography of human embryos: using pronuclear fading rather than insemination in IVF and ICSI cycles removes inconsistencies in time to reach early cleavage milestones. Reprod Biol. 2015;15(2):122–5. doi:10.1016/j.repbio.2015.03.002.CrossRefPubMed

31.

Chang HJ, Lee JR, Jee BC, Suh CS, Kim SH. Impact of blastocyst transfer on offspring sex ratio and the monozygotic twinning rate: a systematic review and meta-analysis. Fertil Steril. 2009;91(6):2381–90. doi:10.1016/j.fertnstert.2008.03.066.CrossRefPubMed

32.

Kirkegaard K, Campbell A, Agerholm I, Bentin-Ley U, Gabrielsen A, Kirk J, et al. Limitations of a time-lapse blastocyst prediction model: a large multicentre outcome analysis. Reprod Biomed Online. 2014;29(2):156–8. doi:10.1016/j.rbmo.2014.04.011.CrossRefPubMed

33.

Freour T, Le Fleuter N, Lammers J, Splingart C, Reignier A, Barriere P. External validation of a time-lapse prediction model. Fertil Steril. 2015;103(4):917–22. doi:10.1016/j.fertnstert.2014.12.111.CrossRefPubMed

Titel: Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer
verfasst von: Daniel Bodri
Satoshi Kawachiya
Takeshi Sugimoto
Jazmina Yao Serna
Ryutaro Kato
Tsunekazu Matsumoto
Publikationsdatum: 01.03.2016
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 5/2016
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0678-4

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Time-lapse variables and embryo gender: a retrospective analysis of 81 live births obtained following minimal stimulation and single embryo transfer

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Purpose

Methods

Results

Conclusions

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Results

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