Background
Alzheimer's disease (AD) is multi-factorial, with genetic and environmental factors implicated in its pathogenesis [
1]. The best-described risk factors for AD are age, a positive family history of dementia, low levels of education, female gender, previous depression, and vascular factors. Interestingly, large epidemiological evidence suggests that Type 2 Diabetes Mellitus (T2DM) is strongly associated with cognitive impairment and dementia due to failure in the action of glucose handling in the neurons as a consequence of impaired brain insulin signaling [
2‐
4]. The deregulated availability of glucose causes a decrease in the rate of energy production necessary for neurons to carry out proper synaptic activity, neuronal survival, and differentiation [
5,
6]. Furthermore, insulin plays a critical role in the brain and is also involved in the phosphorylation of tau [
7,
8], which leads to neurofibrillary tangles, a hallmark of AD-associated neurodegeneration.
The multiple pathophysiological features common to T2DM and AD have paved the way for exploring the beneficial effect of antidiabetic drugs on dementia treatment. Several studies were performed to evaluate the effects of several antidiabetic drugs like incretins, thiazolidines, metformin, and intranasal insulin in the treatment of AD [
9]. Surprisingly, incretins showed the most promising neuroprotective effect [
10], decreasing neuronal apoptosis, oxidative stress, beta-amyloid accumulation, and tau-induced neurofibrillary tangle formation [
11]. Furthermore, several clinical studies demonstrated that glucagon-like peptide-1 receptor agonists (GLP-1RAs) can reduce the risk of dementia in T2DM patients by improving memory and learning and overcoming cognitive impairment [
12]. Recently, a dual glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) receptor agonist, Tirzepatide (TIR), constituting of 39 amino acids, was synthesized [
13]. TIR decreases the glycated hemoglobin level in T2DM patients more efficiently than the GLP-1 RA semaglutide [
14,
15], can significantly improve systemic insulin sensitivity [
16], and is very effective in overcoming insulin resistance [
17].
Surprisingly, both GIP and GLP-1 receptors are expressed in the central nervous system (CNS) and seem to play a role in neural progenitor cell proliferation and behavior modification [
18‐
21]. GIP receptors (GIPRs) have been detected in many areas of the brain, including the hippocampus and hippocampal progenitor cells [
19,
22,
23]. Hippocampus, Purkinje cells, amygdala, substantia nigra, striatum, and several areas of the thalamus, hypothalamus, and brainstem are all positive for GIP mRNA and/or protein [
19,
20,
24]. Thus, it is predicted that a dual agonist of both the incretin receptors might be more efficient in neuroprotection, than a single incretin agonist. Indeed, although GIP and GLP-1 share many common actions in the pancreas, they have been shown to have distinct actions outside of the pancreas [
18], and the potential role of TIR on neuroprotection has never been investigated. More recently, a potential protective effect of TIR against spatial learning and memory impairment has been very recently demonstrated in diabetic mice [
25] but no data on the molecular processes involved were provided.
So far, the effects of the dual GIP/GLP-1 receptor agonist, TIR, on markers of neuronal growth (CREB and BDNF levels), apoptosis (BAX/Bcl2 ratio) differentiation, (pAkt, MAP2, GAP43, and AGBL4) and insulin resistance (GLUT1, GLUT4, GLUT3 and SORBS1) in human neuroblastoma cell line (SHSY5Y) exposed to normal (NG) and high concentration of glucose (HG) for 7 days were investigated. Due to the well-known high glucose-mediated epigenetic changes, the potential role on DNA methylation of genes involved in neuroprotection and epigenetic modulators of neuronal growth (miRNA 34a), apoptosis (miRNA 212) and differentiation (miRNA 29c) was also investigated.
Materials and methods
Cell culture
The SHSY5Y human neuroblastoma cell line was purchased from DSMZ Cell Dive (ACC-209). Cell line has been tested and authenticated following the manufacturer's instruction and it was negative for mycoplasma contamination. The cell line was maintained in an incubator at 37 °C, 95% O
2, and 5% CO
2. The cells were grown in DMEM (Microgem cat# AL007) was used along with 15% fetal bovine serum (FBS, Euroclone cat# ECS0180L), 1%
l-glutamine (Euroclone cat# ECB3000D), 1% penicillin–streptomycin (Euroclone cat# ECB3001D) and 1% non-essential amino acid (Microgem cat# ACL006). The cells were exposed to normal (NG) (25 mM) and high glucose (HG) (150 mM) [
15] (Merck-KGaA, cat# G8644-100ML) for 7 days. Before selecting the appropriate concentration of TIR and initiating experiments, a comprehensive toxicity assessment using cell viability assays and cell counts were performed. Cells exposed to TIR concentrations ranging from 0 µM to 0.4 µM for 7 days exhibited no statistically significant differences in both cell viability percentage and cell numbers (Additional file
1: Fig. S1). Both NG and HG cells, after reaching 70–80% of confluence, were treated with Tirzepatide (Selleckchem, LY3298176 cat# P1206) at concentration of 0.2 μM, since the lower effective dose used in other cell line [
26] was ineffective in our setting. Experiments were executed and repeated at least for 3 times. In all experiments, every 48 h cell culture media was replaced.
Cell proliferation detection
Cell proliferation was assayed by Ki-67 (Alexa Fluor® 488 conjugate) antibody (Cell Signaling cat#11882). According to the manufacturer’s instructions, the cells after 7 days treatment are detached and washed with PBS. The cells were then fixed with 4% formaldehyde followed by incubation for 15 min at room temperature. After washing with PBS, cells were permeabilized using 0.1% PBS-tween on ice for 15 min. According to datasheet 1 × 10
6 cells were incubated with the antibody for 1 h at room temperature after washing with PBS. Before measurement the cells were again washed 2 times and resuspended in PBS. The measurements were carried out using BD Accuri C6 PLUS Personal Flow Cytometer (BD biosciences) and data processing was performed by FlowJo BD Accuri C6 Plus software for windows [
27].
Protein extraction and Western blot analysis
Cells were dissolved in lysis buffer containing protease inhibitors (Tris–HCl, pH 8, 10 mM; NaCl 150 mM; NaF 10 mM; NP40 1%; PMSF 1 mM). Then, the proteins were subjected to 8% or 10% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 μm polyvinylidene fluoride membranes. The membranes were blocked with 5% nonfat milk in TBS-T (Tris-buffered pH8/0.15% Tween 20) at room temperature for 1 h and then incubated with primary antibodies diluted in TBS-T (dilutions in according to data sheet), BDNF (1:1000) (Elabscience cat#e-ab-18244), CREB (1:1000) (Cell Signaling cat#9197), SORBS1(1:500) (Atlas antibodies cat# hpa027559), GAP43 (1:1000) (Abcam cat#ab16053), MAP2 (1:500) (Abcam cat#ab32454), GLUT4 (1:1000) (Abcam cat#ab33780), Bcl-2 (1:1000) (Elabscience cat#e-ab-15522), BAX (1:1000) (Abcam cat#ab32503), GLUT1 (1:1000) (Elabscience cat# e-ab-31556), GLUT3 (1:400) (Abcam cat#ab15311), AGBL4 (1:1000) (Gene Tex cat#n2c3), pAkt (1:1000) (Cell Signaling cat#4060) Akt (Cell Signaling cat# 2920) for overnight at 4 °C. For normalization of protein expression Actin (1:1000) (Abcam cat# ab8227) and Vinculin (1:10,000) (Abcam cat#ab129002) were used as internal control. After three washes in TBS-T, the membranes were incubated with corresponding secondary antibodies, with goat anti-rabbit IgG-h + HRP conjugated (Bethyl cat# A120-101p) and donkey anti-mouse IgG-h-I HRP conjugated (Bethyl cat# A90-137p) (1:5000) secondary antibodies, for 1 h at room temperature. Immunocomplexes were visualized by using Clarity Max Western ECL Substrate (Bio-Rad Laboratories cat#1705062) Immunocomplexes were observed through ChemiDOC Imaging System with Image LAB Software (Bio-Rad Laboratories, Version 6.1). The molecular weight of proteins was estimated with pre-stained protein markers (Opti-Protein-Marker abm cat# G623). Densitometry analysis was performed using ImageJ software.
RNA extraction and miRNA detection
For the isolation and purification of total RNA, including small RNAs from SHSY5Y cells, in the first step, trypsin was used to detach the cells from the flask and collected in 1.5 ml tubes. Then, Trizol and bromocloropropane were added followed by vortexing for 15 s. The tubes were incubated for 15 min at room temperature. After incubation, they were centrifuged for 15 min at 12,000 rpm at 4 °C. The aqueous phase was collected and in which same quantity of chilled isopropanol was added, followed by vortexing. After incubating for more than 1 h at − 80 °C the tubes were again centrifuged for 30 min at 12,000 rpm at 4 °C. The supernatant was discarded, and the pallet was washed with 75% ethanol by centrifugation at 7500 rpm for 10 min. The pallet was resuspended with sterile water and detection of purity and concentration of the RNA were carried out through QIAexpert spectrophotometer (Qiagen cat#1038703). The complementary DNA (cDNA) was synthesized from 2 ng of the total purified RNA by TaqMan MicroRNA Reverse Transcription kit (Applied Biosystem Lithuania cat# 4366597) using specified RT primer (Applied Biosystem cat# 4427975 U6 sn RNA, cat# mir34, cat# mir212 cat# mir29c) following protocol of manufacturer. Rotor-gene Q (Qiagen cat# R0515102) was used to measure the expression of miRNA through utilizing the PrimeTime gene expression master mix (IDT cat#1055772) and the TM primers (Applied Biosystem cat#4427975 U6 snRNA, cat#4440887 mir34a-5p, mir212-3p, mir29c-5p). The PCR condition was polymerase activation at 95 °C for 3 min, followed by 40 cycles of amplification with denaturation at 95 °C for 5 s and annealing at 60 °C for 30 s. All the samples were run in duplicates. For each amplification cycle particular threshold cycle (C
t) value was obtained and the difference between C
t values of targeted miRNAs and U6 were used to calculate ΔC
t. Then to obtain ΔΔC
t difference between the ΔC
t of NG group and drug treated group were taken. Finally, for the analysis of fold change 2
−ΔΔCt was calculated and its average from three individual experiments was plotted in histogram [
28].
Real time PCR
cDNA was synthesized from 1 μg of total RNA isolated from SHSY5Y cells using QuantiTect Reverse Transcription Kit (Qiagen cat# 205310). mRNA levels were determined by qPCR with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad cat#1725270) using Rotor-Gene Q (Qiagen).
Primer’s sequence:
BDNF
Primer 1: 5′-GCTCTGTGCGATTTCATTGTG-3′
Primer 2: 5′-GCCTTCATGCAACCAAAGTATG-3′
GLUT3
Primer 1: 5′-GAAGACTTGAATTAGATTACAGCGATG-3′
Primer 2: 5′-GAAAGAGCCGATTGTAGCAA-3′
GLUT1
Primer 1: 5′-GTGCCATACTCATGACCATCG-3′
Primer 2: 5′-GGCCACAAAGCCAAAGATG-3′
GLUT4
Primer 1: 5′-TCCAACAGATAGGCTCCGAA-3′
Primer 2: 5′-CCCAATGTTGTACCCAAACTG-3′
BAX
Primer 1: 5′-CAAACTGGTGCTCAAGGC-3′
Primer 2: 5′-AAAGATGGTCACGGTCCAAC-3′
BCL2
Primer 1: 5′-GATGACTGAGTACCTGAACCG-3′
Primer 2: 5′-AGCCAGGAGAAATCAAACAGAG-3′
CREB
Primer 1: 5′-GTCCAAACAGTTCAGTCTTCCT-3′
Primer 2: 5′-GTTACACTATCCACTGACTCCTG-3′
AGBL4
Primer 1: 5′-CTTGTAAGCCAGCACCCTAT-3′
Primer 2: 5′-CAGCCAGTGACGATTCAGAT-3′
SORBS1
Primer 1: 5′-ATCTTCCCACCACCTTAAACC-3′
Primer 2: 5′-GAACCACCATCACATTCAGAAC-3′
MAP2
Primer 1: 5′-TGAAGAACATCCGCCACAG-3′
Primer 2: 5′-ATCTTGACATTACCACCTCCAG-3′
GAP43
Primer 1: 5′-AGCCAAGCTGAAGAGAACATAG-3′
Primer 2: 5′-TTCTTAGAGTTCAGGCATGTTCT-3′
β-ACTIN
Primer: 5′-CATCCGCAAAGACCTGTACG-3′
Primer: 5′-CCTGCTTGCTGATCCACATC-3′
All samples were run in duplicate. For each amplification cycle, a threshold cycle (Ct) value was obtained, and ΔCt was calculated as the Ct difference between target mRNA and housekeeping mRNA (β-Actin). Fold increase of mRNA expression compared with NG was calculated using 2−ΔΔCt method.
DNA extraction and methylation analysis
The DNA was isolated with the help of QIAamp DNA Blood Mini kit (Qiagen cat#51104) following the protocols of manufacturer. 350 ug of DNA was taken for bisulfite conversion through EpiTect Fast DNA Methylation Kit (Qiagen cat#59824) according to the instructions from manufacturer. In the next step, this bisulfite converted DNA was amplified using Pyromark PCR kit (Qiagen cat#978703) where the PCR conditions were 1 cycle for 15 min at 95 °C and next 40 cycles for 30 s at 94 °C, 30 s at 56 °C and 10 min at 72 °C. For assurance the PCR products were run in 2% agarose gel electrophoresis (Amersham Bioscience). Then the biotinylate PCR products were investigated for pyrosequencing-based methylation utilizing PyroMark Q48 Advanced CpG Reagent (Qiagen cat# 974022) and commercially designed primers including SORBS1_03 (PM00042168) Chromosome 10, bp 97319534–97321622, AGBL4_03 (PM00094297) Chromosome 1, bp 50488739–50490934, CREB 1 (CRTC1_01) (PM00188804) Chromosome 19, bp 18793114–18795233 and BDNF_01 (PM00155491) Chromosome 11, bp 27721460–27723528. For the analysis PyroMark CpG SW 1.0 software (Qiagen) was used [
29].
Pathway enrichment analysis
QIAGEN Ingenuity Pathway Analysis (IPA) software (QIAGEN, Milan, Italy) was used for enrichment analysis. The ‘‘core analysis’’ function was used to interpret the data based on biological processes, canonical pathways, and gene networks. Each gene identifier was mapped to its corresponding gene object in the Ingenuity Pathway Knowledge Base (IPKB). The p-value of 0.05 was set as the cutoff value for the enrichment. The top five enrichment results of the Molecular and Cellular Function were used as a focus point to connect all the available data using the tools “Connect” and “Path Explorer”.
Statistical analysis
The data were analysed by SPSS IBM Version 23 or GraphPad Prism 7.0 software and expressed as the means ± SEM. Differences between the mean values were considered significant at a p-value of < 0.05. GraphPad Prism software was used for drawing figures.
Discussion
Our study firstly demonstrates that, TIR can affect main molecular pathways involved in neuronal growth (CREB/BDNF), anti-apoptotic process (BAX/Bcl-2), neuro-differentiation (pAkt, MAP2, GAP43 and AGBL4) and neuronal glucose homeostasis (GLUT1, GLUT3 and GLUT4). A role in regulating DNA methylation of genes involved in neuroprotection and epigenetic modulators of neuronal growth (miRNA 34a), apoptosis (miRNA 212), and differentiation (miRNA 29c) was also observed.
Several evidences emphasized the relationship between hyperglycemia and neurodegeneration due to their cross-linking molecular pathways [
33] and the effectiveness of antidiabetic drugs like incretins, thiazolidines, and metformin on preventing neurodegeneration. Among them, incretins showed the most promising neuroprotective effect [
9] reducing the risk of dementia in T2DM patients by improving memory, and learning, and overcoming cognitive impairment [
12]. A novel drug, TIR has been recently synthesized, able to bind both GLP-1 and GIP incretin receptors and with a stronger effect in decreasing glycated hemoglobin and in improving systemic insulin sensitivity in T2DM, when compared to the GLP-1RA [
14,
17]. So far, a potential protective effect against spatial learning and memory impairment has been, only recently, addressed in diabetic mice [
25] but, the molecular processes involved remain unknown.
Our results firstly demonstrated that 7 days of TIR treatment significantly activated the CREB/BDNF signaling cascade and the associated downstream pathways in neuroblastoma cell line (SHSY5Y) exposed to normal (NG) and high glucose (HG) concentration thus suggesting potential implications of TIR in term of neuroprotection and neurodegenerative disorders prevention.
TIR induced an upregulation of protein levels of CREB and BDNF compared to no treated cells (NG) and prevented the pAkt/CREB/BDNF downregulation induced by HG. Interestingly enough, treatment with TIR also prevented the hypermethylation induced by HG (p < 0.05 vs HG) in both CREB and BDNF genes promoter and induced a remarkable reduction in miR-34a expression, a regulator of CREB transcription whose overexpression was observed in aged and neurodegenerative brain, resulting in impaired synaptic plasticity and cognitive performance [
34,
35].
CREB, a member of a large functionally- and structurally related group of transcription factors, has a critical role in nervous system growth and development, synaptic plasticity, long-term memory formation and in the neuroprotective response to pathophysiological effectors [
35‐
38]. The molecular mechanisms by which CREB regulates neuronal survival are centered on the transcription of BDNF, which belongs to a family of neurotrophins that have a crucial role in the survival and differentiation of neurons during development [
39]. The neurotrophins are also involved in plasticity changes related to learning and memory. In the adult brain, BDNF also maintains high expression levels and regulates both excitatory and inhibitory synaptic transmission and activity-dependent plasticity [
40,
41]; in contrast, BDNF deficiency promotes AD progression, dis-homeostasis, neurohormone defects, and the accumulation of neurotoxins [
42].
Recent studies indicate that CREB protects neurons regulating the anti-apoptotic gene B Cell Lymphoma-2 (Bcl-2) expression [
43]. An increase in BAX/Bcl-2 ratio has been shown to result in memory loss and learning capacity deregulation in the cortex and hippocampus region [
44]. Additionally, the phosphorylation of Akt has been associated with the activation of neuronal differentiation markers like microtubule-associated protein-2 (MAP2) growth-associated protein-43 (GAP-43) and the ATP/GTP binding protein-like 4 (AGBL4) [
45‐
48]. These proteins are important targets for treating AD-related tauopathies [
46]. In particular, MAP-2 is a cytoskeletal phosphoprotein mainly associated with microtubules and postsynaptic densities that affect neuronal plasticity; GAP-43 is a presynaptic secreted protein highly expressed during neuronal development and synaptogenesis in the hippocampus and the related cortices [
49‐
52]; AGBL4 takes part in neuronal differentiation by promoting axonal growth and axonal transport of mitochondrion.
Interestingly enough, in our experimental model, TIR treatment was associated with a significant decrease of an important apoptotic prognostic marker, BAX/Bcl2 ratio, and with MAP2, GAP-43 and AGBL4 upregulation in cells exposed to both normal and HG levels. Furthermore, an upregulation of miR-212 and miR-29c, both involved in the regulation of the expression of the mentioned genes, was also observed in both normal and high glucose conditions. miR-212 is transcriptionally regulated by CREB [
53] and its overexpression leads to an increase in Bcl-2 and a reduction in BAX level [
54] and miR-29c directly targets Akt signaling pathway, thereby serving as a neuroprotector. In our cell model, both miRNAs were reduced after 7 days of HG. Thus, the observed effects of TIR on miRNA might partly explain its role in regulating studied molecular pathways. Indeed, the mechanism of action of miRNAs can affect either the transcription (mRNA level) or translation (protein level) [
55] and justify the different trends observed between mRNA and protein levels of some markers analyzed.
According to the experimental evidence that apoptosis is the main mechanism for neuronal death in neurodegenerative diseases and that all the investigated proteins are important targets for treating AD-related abnormalities, our results support the role of TIR as an intriguing therapeutic possibility for the treatment of neurodegenerative disorders beyond its hypoglycemic effects.
Finally, several studies demonstrated that memory processing and cognitive functions depend on neuronal glucose metabolism and sensitivity [
56] and that glucose homeostasis disruption is one of the mechanisms by which T2DM patients develop AD [
57‐
59]. Moreover, hyperglycemia by modulating the expression of glucose transporters induces neuronal insulin resistance [
60]. In the brains of AD patients, a significant reduction in the expression of GLUT1, GLUT3, and GLUT4 was observed, impacting neuronal activity [
61], progressive neuronal loss, and neurodegeneration [
58,
62]. Intriguingly, in our study, we observed that TIR treatment prevented the HG induced mRNA and protein downregulation of GLUT3 along with GLUT1, GLUT4, and SORBS1, a protein that takes part in diabetes pathogenesis [
31], and regulates GLUT4 translocation [
32] affecting neuronal insulin resistance [
63].
Our results agree with previous data showing that TIR significantly activated PI3K/AKT/GSK3β signaling pathway ameliorating insulin resistance in the hippocampus of diabetic rats [
25].
Our results not only confirm the potential role of TIR in the activation of the pAkt/CREB/BDNF downstream signaling cascade but also support its role in neuroprotection and in the prevention of the deleterious effect of hyperglycemia and insulin resistance on the mentioned pathways. Furthermore, the effects observed on CREB and BDNF promoter methylation and in regulating miR-34a, miR-212, and miRNA-29c levels, highlighted the role of TIR in overcoming the epigenetic modifications induced by hyperglycemia.
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