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22.01.2016 | Original Article

TLR4 preconditioning is associated with low success of OK-432 treatment for lymphatic malformations in children

verfasst von: Marc Reismann, Nader Ghaffarpour, Ethel Luvall, Adan Jirmo, Josephine Radtke, Gösta Claesson, Tomas Wester

Erschienen in: Pediatric Surgery International | Ausgabe 5/2016

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Abstract

Purpose

We have recently shown that the relative TLR4 expression on monocytes of low responding pediatric patients after OK-432 treatment is significantly reduced after stimulation with lipopolysaccharide (LPS) compared with high responding children. The aim of this study was to perform further analysis to explain this observation.

Methods

Monocytes from children with high (HR, n = 5) and low response (LR, n = 6) after previous OK-432 treatment were stimulated with LPS for 20 h and analyzed with fluorescence-activated cell sorting (mean fluorescence intensity, MFI; level of significance P ≤ 0.05).

Results

Mean MFI after LPS stimulation was comparable in both groups (HR 1142 ± 652 units, LR 839 ± 427 units, P = 0.85). Significant changes after LPS stimulation are explained by higher pre-stimulation values in the LR group compared with the HR group (950 ± 718 vs. 477 ± 341, P = 0.25) with considerable differences of the mean expression changes after LPS stimulation (HR 665 ± 683 vs. LR −111 ± 605, P = 0.08).

Conclusion

The previously shown reduced TLR4 upregulation on monocytes after LPS stimulation in the LR group compared with the HR group can be primarily explained by TLR preconditioning. This observation implies the use of absolute values with definite thresholds.

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Titel: TLR4 preconditioning is associated with low success of OK-432 treatment for lymphatic malformations in children
verfasst von: Marc Reismann
Nader Ghaffarpour
Ethel Luvall
Adan Jirmo
Josephine Radtke
Gösta Claesson
Tomas Wester
Publikationsdatum: 22.01.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 5/2016
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-016-3865-6

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TLR4 preconditioning is associated with low success of OK-432 treatment for lymphatic malformations in children