Erschienen in:
01.03.2009 | Original Paper
Tyrosine phosphatase beta regulates angiopoietin-Tie2 signaling in human endothelial cells
verfasst von:
Orisha K. Yacyshyn, Patrick F. H. Lai, Kelly Forse, Krystyna Teichert-Kuliszewska, Paul Jurasz, Duncan J. Stewart
Erschienen in:
Angiogenesis
|
Ausgabe 1/2009
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Abstract
Objectives
The endothelial cell (EC)-selective receptor tyrosine kinase, Tie2, and its ligands angiopoietin Ang-1 and Ang-2, are essential for blood vessel maintenance and repair. Ang-1 is an agonist of Tie2 receptor activation, whereas Ang-2 is a context-dependent antagonist/agonist. Therefore, we investigated the role of the EC-selective phosphatase, human protein tyrosine phosphatase beta (HPTPβ), in regulating Tie2 activity.
Methods and results
siRNA silencing of HPTPβ enhanced Ang-1 and Ang-2-induced Tie2 phosphorylation at 10 min (2.5-fold, P < 0.001; and 1.8-fold, P < 0.05, respectively). The cell survival response to Ang-1, but not Ang-2, was enhanced by HPTPβ silencing as measured by flow cytometry (0.85-fold to 0.66-fold, P < 0.05) and ELISA (0.88-fold to 0.53-fold, P < 0.01). Hypoxia, which upregulated HPTPβ expression in endothelial cells, impaired Ang-1-induced Tie2 phosphorylation.
Conclusions
These results reveal a novel role for HPTPβ in modulating Ang-1-Tie2 signaling and endothelial cell survival.