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01.04.2014 | Original Article

Whey protein intake after resistance exercise activates mTOR signaling in a dose-dependent manner in human skeletal muscle

verfasst von: Ryo Kakigi, Toshinori Yoshihara, Hayao Ozaki, Yuji Ogura, Noriko Ichinoseki-Sekine, Hiroyuki Kobayashi, Hisashi Naito

Erschienen in: European Journal of Applied Physiology | Ausgabe 4/2014

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Abstract

Purpose

Protein ingestion after resistance exercise increases muscle protein synthesis (MPS) in a dose-dependent manner. However, the molecular mechanism(s) for the dose-dependency of MPS remains unclear. This study aimed to determine the dose response of mammalian target of rapamycin (mTOR) signaling in muscle with ingestion of protein after resistance exercise.

Methods

Fifteen male subjects performed four sets of six unilateral isokinetic concentric knee extensions. Immediately after exercise, eight subjects consumed water only. The other seven subjects, in a randomized-order crossover design, took either a 10 [3.6 g essential amino acids (EAA)] or 20 g (7.1 g EAA) solution of whey protein. Muscle biopsies from the vastus lateralis muscle were taken 30 min before and 1 h after resistance exercise. Phosphorylation of Akt (Ser473), mTOR (Ser2448), 4E-BP1 (Thr37/46), and S6K1 (Thr389) was measured by western blotting.

Results

Concentric knee extension exercise alone did not increase phosphorylation of Akt and mTOR 1 h after exercise, but ingesting protein after exercise significantly increased the phosphorylation of Akt and mTOR in a dose-dependent manner (P < 0.05). 4E-BP1 phosphorylation significantly decreased after resistance exercise (P < 0.05), but subjects who took 10 or 20 g of protein after exercise showed increased 4E-BP1 from post-exercise dephosphorylation (P < 0.05). S6K1 phosphorylation significantly increased after resistance exercise (P < 0.05), and 20 g of protein further increased S6K1 phosphorylation compared with ingestion of 10 g (P < 0.05).

Conclusions

These findings suggest that whey protein intake after resistance exercise activates mTOR signaling in a dose-dependent manner in untrained men.

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Titel: Whey protein intake after resistance exercise activates mTOR signaling in a dose-dependent manner in human skeletal muscle
verfasst von: Ryo Kakigi
Toshinori Yoshihara
Hayao Ozaki
Yuji Ogura
Noriko Ichinoseki-Sekine
Hiroyuki Kobayashi
Hisashi Naito
Publikationsdatum: 01.04.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Applied Physiology / Ausgabe 4/2014
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-013-2812-7

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Abstract

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Conclusions

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