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Neuroscience Bulletin

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01.04.2016 | Report

Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein

verfasst von: You Yu, Yinan Li, Yan Zhang

Erschienen in: Neuroscience Bulletin | Ausgabe 2/2016

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Abstract

Alzheimer’s disease (AD) is a neurodegenerative disorder in which amyloid β plaques are a pathological characteristic. Little is known about the physiological functions of amyloid β precursor protein (APP). Based on its structure as a type I transmembrane protein, it has been proposed that APP might be a receptor, but so far, no ligand has been reported. In the present study, 9 proteins binding to the extracellular domain of APP were identified using a yeast two-hybrid system. After confirming the interactions in the mammalian system, mutated PLP1, members of the FLRT protein family, and KCTD16 were shown to interact with APP. These proteins have been reported to be involved in Pelizaeus-Merzbacher disease (PMD) and axon guidance. Therefore, our results shed light on the mechanisms of physiological function of APP in AD, PMD, and axon guidance.

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Titel: Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein
verfasst von: You Yu
Yinan Li
Yan Zhang
Publikationsdatum: 01.04.2016
Verlag: Springer Singapore
Erschienen in: Neuroscience Bulletin / Ausgabe 2/2016
Print ISSN: 1673-7067
Elektronische ISSN: 1995-8218
DOI: https://doi.org/10.1007/s12264-016-0021-1

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