nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.11.2012 | ASSISTED REPRODUCTION TECHNOLOGIES

A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles

verfasst von: Ebru Celik, Onder Celik, Banu Kumbak, Ercan Yilmaz, Ilgin Turkcuoglu, Yavuz Simsek, Abdullah Karaer, Yagmur Minareci, Elif Ozerol, Kevser Tanbek

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 11/2012

Einloggen, um Zugang zu erhalten

Abstract

Objective

To determine whether concentrations of oxidative stress markers of follicular fluid and serum are different in GnRH agonist protocol from GnRH antagonist protocol.

Material and method

This was a cross-sectional study. Eighty-four women undergoing controlled ovarian stimulation with either GnRH agonist (n = 39) or GnRH antagonist protocols (n = 45) for IVF/ICSI treatment were assigned by a physician. Blood was obtained at the time of oocyte retrieval, and follicular fluid (FF) from the mature follicles of each ovary was centrifuged and frozen until analysis. Malondialdehyde (MDA), nitric oxide (NO), protein carbonyl (PC), hydroxyl proline (OH-P), sodium oxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) and xanthine oxidase (XO) were assessed in the serum and follicular fluid of each participants.

Results

The mean serum concentrations of GSH-Px, GSH and MDA were lower in the GnRH antagonist group compared to GnRH agonist group, but mean serum SOD was higher in the GnRH antagonist group. The mean follicular SOD, ADA and NO were higher in GnRH antagonist group than GnRH agonist group. The IVF/ICSI outcomes were similar in both groups.

Conclusion(s)

GnRH antagonist protocol is associated with increased oxidative stress. The relation of GnRH analogues with oxidative stress and its implication in follicular growth needs to be addressed in further studies.

Behrman HR, Kodaman PH, Preston SL, Gao S. Oxidative stress and the ovary. J Soc Gynecol Investig. 2001;8:S40–42.PubMedCrossRef

Guérin P, El Mouatassim S, Ménézo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update. 2001;7:175–89.PubMedCrossRef

Ishikawa M. Oxygen radicals-superoxide dismutase system and reproduction medicine. Nippon Sanka Fujinka Gakkai Zasshi. 1993;45:842–8.PubMed

Appasamy M, Jauniaux E, Serhal P, Al-Qahtani A, Groome NP, Muttukrishna S. Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. Fertil Steril. 2008;89:912–21.PubMedCrossRef

Sabatini L, Wilson C, Lower A, Al-Shawaf T, Grudzinskas JG. Superoxide dismutase activity in human follicular fluid after controlled ovarian hyperstimulation in women undergoing in vitro fertilization. Fertil Steril. 1999;72:1027–34.PubMedCrossRef

Sugino N. Reactive oxygen species in ovarian physiology. Reprod Med Biol. 2005;4:31–44.

Vural P, Akgul C, Yildirim A, Canbaz M. Antioxidant defence in recurrent abortion. Clin Chim Acta. 2000;295:169–77.PubMedCrossRef

Tamate K, Sengoku K, Ishikawa M. The role of superoxide dismutase in the human ovary and fallopian tube. J Obstet Gynaecol. 1995;21:401–9.

Setarehbadi R, Hosseinipanah SM, Vatannejad A, Karimi M, Vaisi-raygani A, Tavilani H. Adenosine deaminase activity during menses, follicular and luteal phases of the menstrual cycle. Eur J Obstet Gynecol Reprod Biol. 2011;155:233–4.PubMedCrossRef

10.

Combelles CM, Holick EA, Paolella LJ, Walker DC, Wu Q. Profiling of superoxide dismutase isoenzymes in compartments of the developing bovine antral follicles. Reproduction. 2010;139:871–81.PubMedCrossRef

11.

Yamauchi J, Miyazaki T, Iwasaki S, Kishi I, Kuroshima M, Tei C, Yoshimura Y. Effects of nitric oxide on ovulation and ovarian steroidogenesis and prostaglandin production in the rabbit. Endocrinology. 1997;138:3630–7.PubMedCrossRef

12.

Lee S, Acosta TJ, Nakagawa Y, Okuda K. Role of nitric oxide in the regulation of superoxide dismutase and prostaglandin F (2alpha) production in bovine luteal endothelial cells. J Reprod Dev. 2010;56:454–9.PubMedCrossRef

13.

Engel JB, Griesinger G, Schultze-Mosgau A, Felberbaum R, Diedrich K. GnRH agonists and antagonists in assisted reproduction: pregnancy rate. Reprod Biomed Online. 2006;13:84–7.PubMedCrossRef

14.

Pu D, Wu J, Liu J. Comparisons of GnRH antagonist versus GnRH agonist protocol in poor ovarian responders undergoing IVF. Hum Reprod. 2011;26:2742–9.PubMedCrossRef

15.

Al-Inany HG, Youssef MA, Aboulghar M, Broekmans F, Sterrenburg M, Smit J, Abou-Setta AM. GnRH antagonists are safer than agonists: an update of a Cochrane review. Hum Reprod Update. 2011;17:435.PubMedCrossRef

16.

Akman MA, Erden HF, Tosun SB, Bayazit N, Aksoy E, Bahceci M. Comparison of agonistic flare-up-protocol and antagonistic multiple dose protocol in ovarian stimulation of poor responders: results of a prospective randomized trial. Hum Reprod. 2001;16:868–70.PubMedCrossRef

17.

Choi YS, Ku SY, Jee BC, Suh CS, Choi YM, Kim JG, Moon SY, Kim SH. Comparison of follicular fluid IGF-I, IGF-II, IGFBP-3, IGFBP-4 and PAPP-A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF-embryo transfer patients. Hum Reprod. 2006;21:2015–21.PubMedCrossRef

18.

Devesa M, Martínez F, Coroleu B, Tur R, González C, Rodríguez I, Barri PN. Poor prognosis for ovarian response to stimulation: results of a randomised trial comparing the flare-up GnRH agonist protocol vs. the antagonist protocol. Gynecol Endocrinol. 2010;26:509–15.PubMedCrossRef

19.

Kahraman K, Berker B, Atabekoglu CS, Sonmezer M, Cetinkaya E, Aytac R, Satiroglu H. Microdose gonadotropin-releasing hormone agonist flare-up protocol versus multiple dose gonadotropin-releasing hormone antagonist protocol in poor responders undergoing intracytoplasmic sperm injection-embryo transfer cycle. Fertil Steril. 2009;91:2437–44.PubMedCrossRef

20.

Schmidt DW, Bremner T, Orris JJ, Maier DB, Benadiva CA, Nulsen JC. A randomized prospective study of microdose leuprolide versus ganirelix in in vitro fertilization cycles for poor responders. Fertil Steril. 2005;83:1568–71.PubMedCrossRef

21.

Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34:497–500.PubMed

22.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70:158–69.PubMed

23.

Giusti G. Adenosine deaminase. In: Bergmeyer MV, editor. Methods of enzymatic analysis. 2nd ed. New York: Academic; 1974. p. 1092–8.

24.

Prajda N, Weber G. Malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. FEBS Lett. 1975;59:245–9.PubMedCrossRef

25.

Woessner Jr JF. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys. 1961;93:440–7.PubMedCrossRef

26.

Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990;186:407–21.PubMedCrossRef

27.

Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 1990;186:464–78.PubMedCrossRef

28.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70–7.PubMedCrossRef

29.

Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem. 1990;36:1440–3.PubMed

30.

Baruffi RL, Mauri AL, Petersen CG, Felipe V, Martins AM, Cornicelli J, Cavagna M, Oliveira JB, Franco Jr JG. Recombinant LH supplementation to recombinant FSH during induced ovarian stimulation in the GnRH-antagonist protocol: a meta-analysis. Reprod Biomed Online. 2007;14:14–25.PubMedCrossRef

31.

Albano C, Felberbaum RE, Smitz J, Riethmuller-Winzen H, Engel J, Diedrich K, Devroey P. Ovarian stimulation with HMG: results of a prospective randomized phase III European study comparing the luteinizing hormone-releasing hormone (LHRH)-antagonist and the LHRH-agonist buserelin. Hum Reprod. 2000;15:526–31.PubMedCrossRef

32.

Roulier R, Chabert-Orsini V, Sitri MC, Barry B, Terriou P. Depot GnRH agonist versus the single dose GnRH antagonist regimen (cetrorelix, 3 mg) in patients undergoing assisted reproduction treatment. Reprod Biomed Online. 2003;7:185–9.PubMedCrossRef

33.

de Jong D, Macklon NS, Eijkemans MJ, Mannaerts BM, Coelingh Bennink HJ, Fauser BC, Ganirelix Dose-Finding Study Group. Dynamics of the development of multiple follicles during ovarian stimulation for in vitro fertilization using recombinant follicle stimulating hormone (Puregon) and various doses of the gonadotropin-releasing hormone antagonist ganirelix (Orgalutran/Antagon). Fertil Steril. 2001;75:688–93.PubMedCrossRef

34.

Funston RN, Seidel Jr GE. Gonadotropin-releasing hormone increases cleavage rates of bovine oocytes fertilized in vitro. Biol Reprod. 1995;53:541–5.PubMedCrossRef

35.

Raga F, Casañ EM, Kruessel J, Wen Y, Bonilla-Musoles F, Polan ML. The role of gonadotropin-releasing hormone in murine preimplantation embryonic development. Endocrinology. 1999;140:3705–12.PubMedCrossRef

36.

Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005;3:28.PubMedCrossRef

37.

Zuelke KA, Jeffay SC, Zucker RM, Perreault SD. Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre-implantation stage embryos. Mol Reprod Dev. 2003;64:106–12.PubMedCrossRef

38.

Paszkowski T, Traub AI, Robinson SY, McMaster D. Selenium dependent glutathione peroxidase activity in human follicular fluid. Clin Chim Acta. 1995;236:173–80.PubMedCrossRef

39.

Rahilly M, Carder PJ, al Nafussi A, Harrison DJ. Distribution of glutathione S transferase isoenzymes in human ovary. J Reprod Fertil. 1991;93:303–11.PubMedCrossRef

40.

Toft E, Becedas L, Soderstrom M, Lundqvist A, Depierre JW. Glutathione transferase isoenzyme patterns in the rat ovary. Chem Biol Interact. 1997;108:79–93.PubMedCrossRef

41.

Lasota B, Błaszczyk B, Stankiewicz T, Udała J, Szewczyk M, Matusiak-Bielska D. Superoxide dismutase and glutathione peroxidase activity in porcine follicular fluid in relation to follicle size, birth status of gilts, ovarian location and year season. Acta Scientiarum Polonorum—Zootechnica. 2009;8:19–30.

42.

Comhair SA, Erzurum SC. The regulation and role of extracellular glutathione peroxidase. Antioxid Redox Signal. 2005;7:72–9.PubMedCrossRef

43.

Stouffer RL, Xu F, Duffy DM. Molecular control of ovulation and luteinization in the primate follicle. Front Biosci. 2007;1:297–307.CrossRef

44.

Wen X, Perrett D, Jones N, Tozer AJ, Docherty SM, Iles RK. High follicular fluid adenosine levels may be pivotal in the metabolism and recycling of adenosine nucleotides in the human follicle. Metabolism. 2010;59:1145–55.PubMedCrossRef

45.

Anteby EY, Hurwitz A, Korach O, Revel A, Simon A, Finci-Yeheskel Z, Mayer M, Laufer N. Human follicular nitric oxide pathway: relationship to follicular size, oestradiol concentrations and ovarian blood flow. Hum Reprod. 1996;11:1947–51.PubMedCrossRef

46.

Sugino N, Takiguchi S, Ono M, Tamura H, Shimamura K, Nakamura Y, Tsuruta R, Sadamitsu D, Ueda T, Maekawa T, Kato H. Nitric oxide concentrations in the follicular fluid and apoptosis of granulosa cells in human follicles. Hum Reprod. 1996;11:2484–7.PubMedCrossRef

47.

Rosselli M, Keller PJ, Dubey RK. Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum Reprod Update. 1998;4:3–24.PubMedCrossRef

48.

Amale MH, Shahne AZ, Abavisani A, Nasrollahi S. Effects of inhibiting nitric oxide synthase on cumulus expansion and nuclear maturation of sheep oocytes. Czech J Anim Sci. 2011;56:284–91.

49.

Manau D, Balasch J, Jiménez W, Fábregues F, Civico S, Casamitjana R, Creus M, Vanrell JA. Follicular fluid concentrations of adrenomedullin, vascular endothelial growth factor and nitric oxide in IVF cycles: relationship to ovarian response. Hum Reprod. 2000;15:1295–9.PubMedCrossRef

50.

El-Shahat KH, Kandil M. Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes. Theriogenology. 2012;77:1513–8.PubMedCrossRef

51.

Das S, Chattopadhyay R, Ghosh S, Ghosh S, Goswami SK, Chakravarty BN, Chaudhury K. Reactive oxygen species level in follicular fluid–embryo quality marker in IVF? Hum Reprod. 2006;21:2403–7.PubMedCrossRef

52.

Pasqualotto EB, Agarwal A, Sharma RK, Izzo VM, Pinotti JA, Joshi NJ, Rose BI. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril. 2004;81:973–6.PubMedCrossRef

Titel: A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles
verfasst von: Ebru Celik
Onder Celik
Banu Kumbak
Ercan Yilmaz
Ilgin Turkcuoglu
Yavuz Simsek
Abdullah Karaer
Yagmur Minareci
Elif Ozerol
Kevser Tanbek
Publikationsdatum: 01.11.2012
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 11/2012
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9843-6

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Alter der Mutter beeinflusst Risiko für kongenitale Anomalie

28.05.2024 Kinder- und Jugendgynäkologie Nachrichten

Welchen Einfluss das Alter ihrer Mutter auf das Risiko hat, dass Kinder mit nicht chromosomal bedingter Malformation zur Welt kommen, hat eine ungarische Studie untersucht. Sie zeigt: Nicht nur fortgeschrittenes Alter ist riskant.

Fehlerkultur in der Medizin – Offenheit zählt!

28.05.2024 Fehlerkultur Podcast

Darüber reden und aus Fehlern lernen, sollte das Motto in der Medizin lauten. Und zwar nicht nur im Sinne der Patientensicherheit. Eine negative Fehlerkultur kann auch die Behandelnden ernsthaft krank machen, warnt Prof. Dr. Reinhard Strametz. Ein Plädoyer und ein Leitfaden für den offenen Umgang mit kritischen Ereignissen in Medizin und Pflege.

Mammakarzinom: Brustdichte beeinflusst rezidivfreies Überleben

26.05.2024 Mammakarzinom Nachrichten

Frauen, die zum Zeitpunkt der Brustkrebsdiagnose eine hohe mammografische Brustdichte aufweisen, haben ein erhöhtes Risiko für ein baldiges Rezidiv, legen neue Daten nahe.

Mehr Lebenszeit mit Abemaciclib bei fortgeschrittenem Brustkrebs?

24.05.2024 Mammakarzinom Nachrichten

In der MONARCHE-3-Studie lebten Frauen mit fortgeschrittenem Hormonrezeptor-positivem, HER2-negativem Brustkrebs länger, wenn sie zusätzlich zu einem nicht steroidalen Aromatasehemmer mit Abemaciclib behandelt wurden; allerdings verfehlte der numerische Zugewinn die statistische Signifikanz.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles