A Comparison of Azathioprine and Mycophenolate Mofetil as Adjuvant Drugs in Patients with Pemphigus: A Retrospective Cohort Study

Pemphigus is a group of potentially life-threatening autoimmune mucocutaneous disorders with an incidence of 1–16 new cases per million per year [1, 2]. Autoantibodies targeting different proteins of demosomes, particularly anti-desmoglein (anti-dsg 1) and anti-desmoglein 3 (anti-dsg 3), lead to chronic and severe intra-epidermal blisters. Systemic corticosteroids have long been the mainstay treatment modality, resulting in reductions in the mortality rate from > 70% to < 10% [3]. However, the side effects from prolonged corticosteroid use have driven the search for the best steroid-sparing agent among currently available immunosuppressants, including azathioprine (AZA), mycophenolate mofetil (MMF), cyclophosphamide, cyclosporine, among others [4‐6].

Due to the rarity of the disease, therapeutic prospective controlled trials are limited and management by professionals varies considerably [7]. A majority of guidelines for pemphigus treatment are derived from expert opinions [8‐12]. In the most recent international consensus, rituximab was added as first-line treatment alongside corticosteroids, and AZA and MMF were listed as first-line steroid-sparing agents [12]. The consensus also provides definitions for disease outcome parameters [13], which permit a more accurate evaluation of drug efficacy in clinical studies.

AZA is a synthetic purine analog that is metabolized in the liver to 6-mercaptopurine and its active metabolite 6-thioguanine. This metabolite interferes with DNA replication and suppresses T-cell functions and B-cell antibody production [14]. The therapeutic combination of AZA + corticosteroid for the treatment of pemphigus is recognized as being both effective and safe [15, 16]. A meta-analysis has demonstrated that using AZA as adjuvant therapy with systemic corticosteroids has a steroid-sparing effect when compared to corticosteroid treatment alone [5]. MMF is the prodrug of mycophenolic acid, an active metabolite that can selectively inhibit the de novo purine synthesis specific to lymphocytes [17]; consequently, it is considered to be a more specific treatment than AZA. MMF was introduced into clinical use subsequent to AZA as effective adjuvant treatment for pemphigus and shown to be able to induce remission in patients with pemphigus who relapsed after AZA + corticosteroid combination therapy [18, 19], even in resistant cases [20]. The results of a multicenter randomized control trial that compared the combination MMF + corticosteroid to corticosteroid therapy alone led the authors to conclude that while there were no differences in the percentages of patients achieving absence of lesions, the group treated with the MMF + cortcosteroid combination showed faster and more durable responses than the prednisolone-only group [21]. This result conflicts with that reported in another study involving newly diagnosed patients, with the authors reporting no superiority of MMF + cortcosteroid combination therapy in terms of response to treatment or prevention of relapses over corticosteroid monotherapy [22]. Although AZA and MMF are widely accepted immunosuppressants, only a few studies have directly compared these two drugs, and these studies were conducted before the publication of standardized endpoints [23, 24].

Reliable data on whether AZA or MMF differ in efficacy may be valuable, especially in financially restrained countries where rituximab is not readily available or affordable and combination treatment with corticosteroids and adjuvant immunosuppressants are the only option. Thus, the aim of our study was to compare the efficacy and safety of each of these agents as adjuvant therapy with the corticosteroid prednisolone (AZA + prednisolone vs. MMF + prednisolone) for the treatment of pemphigus, using the recommended outcome parameters.

This retrospective study was approved by the Mahidol University Institutional Review Board (ID 08-60-74) and conducted at the Autoimmune Blistering Skin Diseases Clinic, Division of Dermatology, Ramathibodi Hospital, Bangkok, Thailand. All procedures performed in studies involving human participants were in accordance with the institutional research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

Medical records of patients with pemphigus treated at our clinic between January 2007 and July 2017 were retrieved and analyzed. Inclusion criteria were: age > 15 years; diagnosis of pemphigus vulgaris (PV) or pemphigus foliaceus (PF) based on the typical clinical presentations, histopathology, immunofluorescence studies and enzyme-linked immunosorbent assays (ELISA) for the anti-desmoglein autoantibodies anti-dsg1 and anti-dsg3; and treatment with prednisolone in combination with either AZA or MMF as mono-adjuvant therapy. Exclusion criteria were: coexisting autoimmune diseases; multiple drug use; inconsistency with follow-up evaluations; and pregnancy or lactation. For each subject, the baseline characteristics, disease duration, clinical presentations and disease severity assessment using the Autoimmune Bullous Skin Disorder Intensity Score (ABSIS) at the start of the combination therapy and throughout subsequent follow-ups were documented. Patients were divided into one of two treatment groups, receiving either AZA or MMF as adjuvant therapy with prednisolone, and treated in parallel. The treatment response, steroid-sparing effect, relapse rate and adverse events of the two groups were compared.

Of the 98 patients with PV or PF included in this study, 28 had received or were receiving multiple immunosuppressants or other treatment modalities, such as, for example, intravenous immunoglobulin, plasmapheresis and/or rituximab, and therefore did not fulfil the inclusion criteria. Eight patients were excluded due to inconsistency regarding the follow-up visits. Of the 62 remaining patients, 37 patients were treated with AZA and 25 patients with MMF as adjuvant therapy with prednisolone. Overall, the baseline characteristics, type of pemphigus, extent of disease, disease duration, follow-up duration, ABSIS and anti-dsg 1/anti-dsg 3 at baseline did not differ between the two groups (Table 1). The initial mean doses of prednisolone in the AZA and MMF groups were also similar (0.53 vs. 0.48 mg/kg/day, respectively; p = 0.479). In all cases, prednisolone was gradually tapered down according to the individual’s clinical response under the management of one expert dermatologist. In the AZA group, patients received AZA at a mean initial dose of 0.83 mg/kg/day, which was then titrated up to a mean maximum dose of 1.41 mg/kg/day. In the MMF group, patients received MMF at a mean initial dose of 910 mg/day, which was then titrated up to a mean maximum dose of 1530 mg/day.

A majority of patients in both groups (AZA group 88.2%; MMF group 71.4%) reached the early endpoint (ECP), with no significant between-group difference (p = 0.156) (Table 2); the median time required to achieve this early endpoint was also comparable [AZA: 2.9 months, 95% confidence interval (CI) 2.1, 4.4; MMF: 3.5 months, 95% CI 1.6, 5.9; p = 0.362) (Fig. 1).

CR when on therapy was observed in 73 and 72% of patients receiving AZA and MMF as adjuvant, respectively (p = 0.933) (Table 2). However, the median time to achieve CR on therapy was significantly shorter in the MMF group than in the AZA group (7.3 vs. 12.5 months; p = 0.019) (Fig. 2). The hazard ratio for CR on therapy for the MMF group compared to the AZA group was 2.07 (95% CI 1.11, 3.86) within the follow-up period (p = 0.021; Table 3). After adjusting for all relevant confounding factors, including baseline ABSIS, age, diagnosis (PV, PF), type of patient (new, partially treated, relapsed), extent of involvement and presence of complications, the hazard ratio for CR on therapy for the MMF group compared to the AZA group was 2.55 (95% CI 1.15, 5.63) and remained significant (p = 0.021). Subgroup analysis showed that patients with PV, but not those with PF, who received MMF had a shorter time to achieve CR on therapy than did patients receiving AZA (6.5 vs. 14.4 months), but this difference did not reach statistical significance (p = 0.093). For CR off therapy, the rates were 24.3 and 20% in AZA group and MMF group, respectively (p = 0.690) (Table 2). The median time to achieve this endpoint appeared to be shorter for the MMF group than for the AZA group (29.5 vs. 52.3 months), but the difference was not statistically significant (p = 0.110) (Fig. 3). Immunological remission was detected in approximately one-third of patients in each group, with no significant between-group difference (p = 0.913). Although the median time to immunological remission was shorter in the MMF versus the AZA group (15.3 vs. 41.9 months), this difference did not reach statistical significance (p = 0.157) (Fig. 4).

The chronic autoimmune nature of pemphigus requires longstanding treatment with corticosteroids, which often leads to severe adverse events [3]. Combination therapy with various adjuvants has long been a subject of interest and consistently included in previous national and international treatment guidelines [8‐12]. AZA (1–3 mg/kg/day) and MMF (30–45 mg/kg/day) are both listed as “first line adjuvants” in the European international guideline [11] and as “first line corticosteroid sparing agents” in the recent international expert panel recommendations [12].

Numerous studies have compared corticosteroid with either AZA or MMF as adjuvant (combination therapy) with corticosteroid monotherapy, but only a few studies have directly compared these two combination therapeutic modalities [23‐25]. In terms of treatment response, our results suggest that while patients receiving corticosteroid in combination with AZA or MMF achieved similarly high rates of remission, MMF was associated with better outcomes in terms of a significantly shorter time to CR on therapy and a trend towards shorter time to CR off therapy. These results are contradictory to those reported in a previous study in which patients on AZA were shown to have a significantly shorter mean duration to remission (defined as complete re-epithelization of all previous lesions) [23]. This difference may be explained by the difference in the outcome parameters. The endpoints used in this earlier study may actually be more comparable to the time required to achieve the ECP in our study; we also found this endpoint (defined as an early endpoint in our study) to be shorter in the AZA group than in the MMF group, but the difference was not statistically significant. The shorter time to CR in the patients receiving MMF in our study also translated into a significantly lower cumulative steroid dose in this group. This result highlights in particular the superiority of MMF over AZA as these adjuvant immunosuppressants were prescribed primarily to reduce the use of corticosteroid. In contrast to our findings, Chams-Davatchi et al. reported a lower mean total dose of prednisolone administered in the AZA group when compared to the MMF group during their treatment period of 1 year [24]. However, in their study the cumulative corticosteroid dose was calculated after a fixed period of 1 year, whereas in our study the total cumulative dose was calculated up to the time of CR.

Our results appear to indicate that the recurrence rate was higher in the AZA group, but the statistical significance of the difference could not be determined due to the small number of recurrences and the difference in number of patients achieving the ECP in each group. In two previous studies, the rate of and time to recurrence were similar in patients receiving AZA or MMF [23, 25]. Our results show that the rate and type of adverse event did not differ between the two treatment groups, a finding consistent with results reported previously [23, 24]. Like earlier studies, the majority of adverse events were minor, with candidiasis being the most common [24], and findings of elevated liver enzymes were also prevalent, with a slightly higher proportion observed in the AZA group [23].

There are a few limitations to our study. Due to the retrospective design, some data in the electronic records may have been incomplete and the follow-up intervals may not be uniform in all patients. Although the patients were treated in the same clinic by the same expert dermatologist, tapering of both corticosteroid and AZA/MMF may have varied between individuals. However, our data reflect real-life scenarios and demonstrate the performance of each adjuvant when given in clinical practice. In the future, findings from prospective controlled trials with standardized endpoints may support these results.

The results of this study suggest that corticosteroid in combination with either AZA or MMF is an effective treatment regimen for pemphigus, based on similarly high rates of patients in either treatment group achieving remission. However, MMF was associated with a significantly shorter time to achieve CR on therapy and a significantly higher steroid-sparing effect, indicating a higher potential for lowering the risk of adverse events associated with prolonged steroid use. Further prospective controlled trials with standardized disease outcomes are required to confirm this superiority over AZA.